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Study of the toxic effect of trichothecene mycotoxin produced by Fusarium sporotrichioides in an experiment on pigs

https://doi.org/10.18470/1992-1098-2022-1-62-79

Abstract

Aim. To assess the consequences of long‐term consumption by pigs of feed experimentally contaminated with T‐2 toxin in an amount close to the most commonly found in feed.

Material and Methods. The experiments were carried out on pigs, which were fed food contaminated with T‐2 toxin in the amount of 200 mkg/kg for 45 days. During the experiment, the clinical condition of animals, feed and water consumption, the dynamics of live weight, analysis of blood parameters, the state of internal organs and tissues and statistical processing of the data obtained by conventional methods were undertaken.

Results. Long‐term consumption of feed contaminated with mycotoxin T‐2 by pigs did not cause a pronounced clinical picture of toxicosis. However, the treated animals had diarrhea and lag in growth and development. According to the results of the blood test, it was concluded that the toxin suppressed the hematopoiesis process, inhibited protein synthesis, reduced resistance and microphagocytic function of the body, metabolic disorders and the functioning of most organs and systems. Pathological conditions and degenerative changes of cells and tissues of internal organs were recorded and mediated by the initiation of oxidative stress, inflammatory processes and cytotoxic effect.

Conclusions. The consumption of feed contaminated with T‐2 toxin in a dose of 200 mkg /kg by pigs was accompanied by disturbances of metabolic processes, functions of organs and systems, manifested by a decrease in growth intensity, changes in the blood picture, activity of enzyme systems, induction of oxidative stress, immunosuppressive and inflammatory reactions and pathomorphological disorders. Thus, the oral intake of T‐2 toxin, even in small concentrations, was found to have a complex negative effect on the body.

About the Authors

I. I. Idiyatov
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Ilgiz I. Idiyatov, Candidate of Biological Sciences, Head of the Department, Department of Toxicology

Nauchniy Gorodok‐2, Kazan, 420075
Tel. +79274459046



I. R. Kadikov
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Ilnur R. Kadikov

Kazan



V. R. Saitov
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Vadim R. Saitov

Kazan



G. S. Kashevarov
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Gleb S. Kashevarov

Kazan



I. M. Fitsev
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Igor M. Fitsev

Kazan



A. M. Tremasova
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Anna M. Tremasova

Kazan



K. V. Perfilova
Federal Center for Toxicological, Radiation and Biological Safety
Russian Federation

Ksenia V. Perfilova

Kazan



M. M. Salnikova
Kazan (Volga region) Federal University
Russian Federation

Marina M. Salnikova

Kazan



References

1. Bryden W.L. Mycotoxin contamination of the feed supply chain: implications for animal productivity and feed security // Animal Feed Sciens Technology. 2012. V. 173. P. 134‐158. DOI: 10.1016/j.anifeedsci.2011.12.014

2. Idiyatov I.I., Tremasova A.M., Tremasov Y.M., Valiullin L.R., Kalinitchenko V.P., Rud V.Y., Semenov A.M. Screening of endophytic bacteria exhibiting anthagonistic activity against Fusarium sporotrichioides micromycete // IOP Conference Series Earth and Environmental Science. 2021. V. 663(1). Article number: 012047. DOI: 10.1088/1755‐1315/663/1/012047.

3. Gruber‐Dorninger C., Jenkins T., Schatzmayr G. Global mycotoxin occurrence in feed: a ten‐year survey // Toxins. 2019. V. 11(7). 375 p. DOI: 10.3390/toxins11070375

4. Kalantari H., Moosavi M. Review on T‐2 toxin // Jundishapur Journal of Natural Pharmaceutical Products. 2010. V. 5(1). P. 26‐38. https://scholar.google.com/scholar_lookup?journal=Jundishapur+J+Nat+Pharm+Prod.&title=Review+on+T‐2+Toxin.&author=H+Kalantari&author=M+Moosavi&volume=5&issue=1&publication_year=2010&pages=26‐38& (accessed: 17.10.2021)

5. Khabirova S.R., Idiyatov I.I., Shuralev E.A., Tremasova A.M. Metabolite‐associated enzymatic properties of microorganisms with an antagonistic effect on Aspergillus and Fusarium fungi, pathogenic for crops and farm animals // IOP Conference Series Earth and Environmental Science. 2021. V. 723. Article number: 022022. DOI: 10.1088/1755‐1315/723/2/022022

6. Alassane‐Kpembi I., Puel O., Oswald I.P. Toxicological interactions between the mycotoxins deoxynivalenol, nivalenol and their acetylated derivatives in intestinal epithelial cells // Archives of Toxicology. 2015. V. 89(8). P. 1337‐1346. DOI: 10.1007/s00204‐014‐1309‐4

7. Bryden W.L. Mycotoxins and mycotoxicoses: significance, occurrence and mitigation in the food chain. In: Ballantyne B., Marrs T., Syversen T. // General and Applied Toxicology. 2009. 3rd ed. Chichester, UK: John Wiley and Sons Ltd. P. 3529‐3553.

8. Afsah‐Hejri L., Jinap S., Hajeb P., Radu S., Shakibazadeh S.H. A review on mycotoxins in food and feed: Malaysia case study // Comprehensive Reviews in Food Science and Food Safety. 2013. V. 12(6). P. 629‐651. DOI: 10.1111/1541‐4337.12029

9. Idiyatov I.I. Sochetannoe vozdeistvie malykh doz dioksina i t‐2 toksina na organizm porosyat i puti korrektsii [The combined effect of small doses of dioxin and T‐2 toxin on the body of piglets and ways of correction]. Dissertatsiya na soiskanie uchenoi stepeni kandidata biologicheskikh nauk. Kazan', 2013, 172 р. (In Russian)

10. Kanora A., Maes D. The role of mycotoxins in pig reproduction: A review // Veterinarni Medicina. 2009. V. 54(12). P. 565‐576. DOI: 10.17221/156/2009‐VETMED

11. Obremski K., Podlasz P., Żmigrodzka M., Winnicka A., Woźny M., Brzuzan P., Jakimiuk E., Wojtacha P., Gajęcka M., Zielonka Ł., Gajęcki M. The effect of T‐2 toxin on percentages of CD4+, CD8+, CD4+CD8+ and CD21+ lymphocytes, and mRNA expression levels of selected cytokines in porcine ileal Peyer’s patches // Polish Journal of Veterinary Sciences. 2013. V. 16(2). P. 341‐349. DOI: 10.2478/pjvs‐2013‐0046

12. Omar H.M. Mycotoxins‐induced oxidative stress and disease // Mycotoxin and Food Safety in Developing Countries. 2013. Chapter 3. P. 63‐92. INTECH Europe Publishers, Croatia. DOI: 10.5772/51806

13. Adhikari M., Negi B., Kaushik N., Adhikari A., Al‐Khedhairy A.A., Kaushik N.K., Choi E.H. T‐2 mycotoxin: toxicological effects and decontamination strategies // Oncotarget. 2017. V. 8 (20). Р. 33933‐33952. DOI: 10.18632/oncotarget.15422

14. Pierron A., Alassane‐Kpembi I., Oswald I.P. Impact of mycotoxin on immune response and consequences for pig health // Animal Nutrition. 2016. V. 2 (2). P. 63‐68. DOI: 10.1016/j.aninu.2016.03.001

15. Oswald I.P., Marin D.E., Bouhet S., Pinton P., Taranu I., Accensi F. Immunotoxicological risk of mycotoxins for domestic animals // Food Additives and Contaminants. 2005. V. 22(4). P. 354‐360. DOI: 10.1080/02652030500058320

16. Alassane‐Kpembi I., Schatzmayr G., Taranu I., Marin D., Puel O., Oswald I.P. Mycotoxins co‐contamination: methodological aspects and biological relevance of combined toxicity studies // Critical Reviews in Food Science and Nutrition. 2017. V. 57(16). P. 3489‐3507. DOI: 10.1080/10408398.2016.1140632

17. European Commission. Commission Recommendation No 2013/165/EU of 27 March 2013 on the presence of T‐2 and HT‐2 toxin in cereals and cereal products // Official Journal of the European Union. 2013. OJ L 91. P. 12‐15. https://eur‐lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2013:294:0044:0044:EN:PDF (accessed: 17.10.2021)

18. Chauhan R.S., Agarwal D.K. Textbook of Veterinary Clinical and Laboratory Diagnosis, 2nd Edition. 2008. 364 p. https://www.academia.edu/32745221/Textbook_of_Veterinary_Clinical_and_Laboratory_Diagnosis_2nd_Edition_Vetbooks_ir. (accessed: 17.10.2021)

19. Saitov V.R. Toksikologicheskii i ul'trastrukturnyi analiz izmenenii v organizme i kletkakh zhivotnykh pri khronicheskom vozdeistvii ksenobiotikov i primenenii lechebno‐profilakticheskikh sredstv [Toxicological and ultrastructural analysis of changes in the body and cells of animals under chronic exposure to xenobiotics and the use of therapeutic and prophylactic agents]. Dissertatsiya na soiskanie uchenoi stepeni doktora biologicheskikh nauk. Kazan', 2014, 350 р. (In Russian)

20. JCGM 100:2008 Evaluation of Measurement Data – Guide to the Expression of Uncertainty in Measurement. https://www.bipm.org/fr/search?p_p_id=search_portlet&p_p_lifecycle=2&p_p_state=normal&p_p_mode=view&p_p_resource_id=%2Fdownload%2Fpublication&p_p_cacheability=cacheLevelPage&_search_portlet_dlFileId=41373434&p_p_lifecycle=1&_search_portlet_javax.portlet.action=search&_search_portlet_formDate=1618209355352&_search_portlet_query=JCGM+100%3A2008%2C+Evaluation+of+measurement+data+%E2%80%94+Guide+to+the+expression+of+uncertainty+in+measurement&_search_portlet_source=BIPM (accessed: 17.10.2021)

21. Wu J., Ordinario T., Qin F. Multiclass‐multianalyte method for mycotoxin testing in foods using immunoaffinitycolumn sampleclean‐upwith QSight LC/MS/MS. 2020. https://www.perkinelmer.com/lab‐solutions/resources/docs/app‐145410‐mycotoxins‐in‐food‐by‐iac‐lcmsms.pdf (accessed: 17.10.2021)

22. Sagawa N., Takino T., Kurogochi S. A simple method with liquid chromatography / tandem mass spectrometry for the determination of the six trichothecene mycotoxins in rice medium // Bioscience, Biotechnology and Biochemistry. 2006. V. 70(1). P. 230‐236. DOI: 10.1271/bbb.70.230

23. Baere S.D., Goossens J., Osselaere A., Devreese M., Vandenbroucke V., Backer P.D., Croubels S. Quantitative determination of T‐2 toxin, HT‐2 toxin, deoxynivalenol and deepoxy‐deoxynivalenol in animal body fluids using LC‐MS/MS detection // Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 2011. V. 879. P. 2403‐2415. DOI: 10.1016/j.jchromb.2011.06.036

24. Yiannikouris A., Jouany J.P. Mycotoxins in feeds and their fate in animals: a review // Animal Research. 2002. V. 51(2). P. 81‐99. DOI: 10.1051/animres:2002012

25. Nayakwadi S., Ramu R., Sharma A.К., Gupta V.К., Rajukumar K., Kumar V., Shirahatti P.S., Rashmi L., Basalingappa K.M. Toxicopathological studies on the effects of T‐2 mycotoxin and their interaction in juvenile goats // PLoS One. 2020. V. 15(3). e0229463. DOI: 10.1371/journal.pone.0229463

26. Ferreras M.C., Benavides J., García‐Pariente C., Delgado L., Fuertes M., Muñoz M., García‐Marín J.F., Pérez V. Acute and chronic disease associated with naturally occurring T‐2 mycotoxicosis in sheep // Journal of Comparative Pathoogy. 2013. V. 148. P. 236‐242. DOI: 10.1016/j.jcpa.2012.05.016

27. Tarasova E.Yu., Matrosova L.E., Tanaseva S.A., Mishina N.N., Potekhina R.M., Ermolaeva O.K., Smolentsev S.Yu., Tremasova A.M., Kadikov I.R., Egorov V.I., Aslanov R.M., Semenov E.I. Protective effect of adsorbent complex on morphofunctional state of liver during chicken polymycotoxicosis // Systematic Reviews in Pharmacy. 2020. V. 11(11). P. 264‐268. DOI: 10.31838/srp.2020.11.38

28. Аdhikari M., Negi B., Kaushik N., Adhikari A., Al‐Khedhairy A.A., Kaushik N.K., Choi E.H. T‐2 mycotoxin: toxicological effects and decontamination strategies // Oncotarget. 2017. V. 8(20). Р. 33933‐33952. DOI: 10.18632/oncotarget.15422

29. Devreese M., Backer P.D., Croubels S. Different methods to counteract mycotoxin production and its impact on animal health // Vlaams Diergeneeskundig Tijdschrift. 2013. V. 82. РР. 181‐190.

30. Gentry P.A., Ross M.L., Chan P.K. Effect of T‐2 toxin on bovine haematological and serum enzyme parameters // Veterinary and Human Toxicology. 1984. V. 26(1). Р. 24‐28. https://pubmed.ncbi.nlm.nih.gov/6702098/ (accessed: 17.10.2021)

31. Gentry P.A., Cooper M.L. Effect of Fusarium T‐2 toxin on haematological and biochemical parameters in the rabbit // Canadian Journal of Comparative Medicine. 1981. V. 45(4). Р. 400‐405.

32. Hartl F.U. Molecular chaperones in cellular protein folding // Nature. 1996. V. 381(6583). Р. 571‐579. DOI: 10.1038/381571a0

33. Wan Q., Wu G., He Q., Tang H., Wang Y. The toxicity of acute exposure to T‐2 toxin evaluated by the metabonomics technique // Molecular BioSystems. 2015. V. 11(3). Р. 882‐891. DOI: 10.1039/C4MB00622D

34. Pande V.V., Kurkure N.V., Bhandarkar A.G. Effect of T‐2 toxin on growth, performance and haematobiochemical alterations in broilers // Indian Journal of Experimental Biology. 2006. V. 44(1). Р. 86‐88. https://pubmed.ncbi.nlm.nih.gov/16430098/ (accessed: 17.10.2021)

35. Zhang Y., Han J., Zhu C.C., Tang F., Cui X.S., Kim N.H., Sun S.C. Exposure to HT‐2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes // Scientific Reports. 2016. V. 6. Article number: 33904. DOI: 10.1038/srep33904

36. Sehata S., Kiyosawa N., Atsumi F., Ito K., Yamoto T., Teranishi M., Uetsuka K., Nakayama H., Doi K. Microarray analysis of T‐2 toxininduced liver, placenta and fetal liver lesions in pregnant rats // Experimental and Toxicologic Pathology. 2005. V. 57(1). Р. 15‐28. DOI: 10.1016/j.etp.2005.02.005

37. Prabhu P.C., Dwivedi P., Sharma A.K. Toxicopathological studies on the effects of aflatoxin B(1), ochratoxin A and their interaction in New Zealand White rabbits // Experimental and Toxicologic Pathology. 2013. V. 65(3). Р. 277‐286. DOI: 10.1016/j.etp.2011.09.003

38. Zhuang Z., Yang D., Huang Y., Wang S. Study on the Apoptosis Mechanism Induced by T‐2 Toxin // PLoS ONE. 2013. V. 8(12). e83105. DOI: 10.1371/journal.pone.0083105

39. Do i K., Uetsuka K. Mechanisms of mycotoxin‐induced neurotoxicity through oxidative stress‐associated pathways // International Journal of Molecular Sciences. 2011. V. 12(8). Р. 5213‐5237. DOI: 10.3390/ijms12085213

40. Chaudhari M., Jayaraj R., Bhaskar A.S., Lakshmana Rao P.V. Oxidative stress induction by T‐2 toxin causes DNA damage and triggers apoptosis via caspase pathway in human cervical cancer cells // Toxicology. 2009. V. 262(2). Р. 153‐161. DOI: 10.1016/j.tox.2009.06.002

41. Chaudhari M., Jayaraj R., Santhosh S.R., Rao, P.V. Oxidative damage and gene expression profile of antioxidant enzymes after T‐2 toxin exposure in mice // Journal of Biochemical and Molecular Toxicology. 2009. V. 23(3). Р. 212‐221. DOI: 10.1002/jbt.20282

42. Quiroga M.A., Risso M.A., Perfumo C.J. T‐2 mycotoxin intoxication in piglets: a systematic pathological approach and apoptotic immunohistochemical studies // Brazilian Journal of Veterinary Pathology. 2009. V. 2. Р. 16‐22. https://bjvp.org.br/wp‐content/uploads/2015/07/V.2‐N.1‐06‐20881_2009_12_30_23_50.pdf. (accessed: 17.10.2021)

43. Guilford F.T., Hope J. Deficient Glutathione in the Pathophysiology of Mycotoxin‐Related Illness // Toxins. 2014. V. 6(2). Р. 608‐623. DOI: 10.3390/toxins6020608

44. Chen J., Chu Y., Cao J., Yang Z., Guo X., Wang Z. T‐2 toxin induces apoptosis, and selenium partly blocks, T‐2 toxin induced apoptosis in chondrocytes through modulation of the Bax/Bcl‐2 ratio // Food Chemical Toxicology. 2006. V. 44(4). Р. 567‐573. DOI: 10.1016/j.fct.2005.09.004

45. Wang G.Y., Zhang J.W., Lu Q.H., Xu R.Z., Dong, Q.H. Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation // Journal of Zhejiang University Science. 2007. V. 8(4). Р. 248‐255. DOI: 10.1631/jzus.2007.B0248

46. Antonissen G., Martel A., Pasmans F., Ducatelle R., Verbrugghe E., Vandenbroucke V., Li S., Haesebrouck F., Immerseel, F., Croubels S. The impact of Fusarium mycotoxins on human and animal host susceptibility to infectious diseases // Toxins. 2014. V. 6(2). Р. 430‐452. DOI: 10.3390/toxins6020430

47. Jaradat Z.W. T‐2 mycotoxin in the diet and its effects on tissues // In: Reviews in Foodand Nutrition Toxicity, Watson R.R., Preedy V.R. (Eds). Boca Raton, CRC Press. 2005. V. 4. Р. 173‐212. DOI: 10.1201/9781420037524.ch7

48. Obremski K., Zielonka L., Gajecka M., Jakimiuk E., Bakuła T., Baranowski M., Gajecki M. Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T‐2 toxin and zearalenone in the pig // Polish Journal of Veterinary Sciences. 2008. V. 11(4). Р. 339‐345. https://pubmed.ncbi.nlm.nih.gov/19227132/ (accessed: 17.10.2021)

49. Quiroga M.A., Miguel A.R., Carlos J.P. T‐2 mycotoxin intoxication in piglets: a systematic pathological approach and apoptotic immune‐histochemical studies // Brazilian Journal of Veterinary Pathology. 2009. V. 2(1). Р. 16‐22. http://bjvp.org.br/wp‐content/uploads/2015/07/V.2‐N.1‐06‐20881_2009_12_30_23_50.pdf. (accessed: 17.10.2021)

50. Nagata T., Suzuki H., Ishigami N., Shinozuka J., Uetsuka K., Nakayama H., Do i K. Development of apoptosis and changes in lymphocyte subsets in thymus, mesenteric lymph nodes and Peyer’s patches of mice orally inoculated with T‐2 toxin. // Experimental and Toxicologic Pathology. 2001. V. 53(4). Р. 309‐315. DOI: 10.1078/0940‐2993‐00196

51. Wu Q., Dohnal V., Huang L., Kuca K., Yuan Z. Metabolic pathways of trichothecenes // Drug Metabolism Reviews. 2010. V. 42(2). Р. 250‐267. DOI: 10.1080/03602530903125807

52. Mukhammadiev Rin.S., Mukhammadiev Rish.S., Biryulya V.V., Idiyatov I.I., Nabatov A.A., Glinushkin A.P., Valiullin L.R. An in vitro estimation of cytotoxicity of fusarium trichothecene on the breast cancer cell line. Siberian Journal of Oncology, 2019, vol. 18(6), pp. 90‐95. (In Russian) DOI: 10.21294/1814‐4861‐2019‐18‐6‐90‐95

53. Idiyatov I.I., Valiullin L.R., Biryulya V.V., Shangaraev N.G., Raginov I.S., Tremasov M.I., Lekishvili M.V., Nikitin A.I. Cytotoxic activity of T‐2 toxin for a immortalized of cattle fetal lung epithelium cells. Genes and cells, 2017, vol. 12(1), pp. 41‐46. (In Russian) DOI: 10.23868/201703006


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Idiyatov I.I., Kadikov I.R., Saitov V.R., Kashevarov G.S., Fitsev I.M., Tremasova A.M., Perfilova K.V., Salnikova M.M. Study of the toxic effect of trichothecene mycotoxin produced by Fusarium sporotrichioides in an experiment on pigs. South of Russia: ecology, development. 2022;17(1):62-79. (In Russ.) https://doi.org/10.18470/1992-1098-2022-1-62-79

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