CYTOGENETIC CHANGES IN CELLS OF THE APICAL MERISTEM OF GREEN PLANTING OF THE CITY DEPENDING ON THE LEVEL OF ANTHROPOGENIC LOAD
https://doi.org/10.18470/1992-1098-2017-3-146-152
Abstract
Aim. The aim of the research is to study the cytogenetic disorders in the cells of apical meristem of the sprouts of urban green plantations of the small-leaved linden (Tilia cordata L.) and rattlertree (Populus alba L.) growing under different conditions in terms of anthropogenic impact as parks, squares and places of street plantings along urban highways. Methods. Cytogenetic abnormalities in the cells of the apical meristem of small-leaved linden and rattlertree have been studied in terms of the frequency of occurrence of micronuclei and protrusions of nuclei of different forms. The buds for analysis were collected from a tree of 1.5-2 m. height in spring during March-April 2009, 2012 and 2015. The collected buds were processed with acetalcohol, micropreparations of cells of the apical meristem were made and stained with acetocarmine. Karyological indicators were analyzed and cited by L.P. Sychova. Findings. The study reveals that the most pronounced cytogenetic disturbances in terms of the frequency of occurrence of micronuclei and protrusions of nuclei of different forms were significantly higher in the cells of the apical meristem of the sprouts of small-leaved linden and rattlertree plantations along the city's highways compared to the same parameters of the parks and squares studied. Cells of the apical meristem of the sprouts of small-leaved linden are most sensitive to technogenically polluted environment in comparison with the cells of rattlertree sprouts. Conclusion. Cells of the apical meristem of the sprouts of the investigated trees, especially of small-leaved linden, can serve as indicators of the genotoxicity of the city's environment.
About the Authors
Galina G. Ladnova
Orel State University named after I.S. Turgenev
Russian Federation
Doctor of Biological Sciences, Professor, Lead of the Department of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev", tel. 89202817861, 95 Komsomolskaya st., Orel, Russia. E-mail: gladnova@yandex.ru other IDs: Scopus Author ID:6602758422, Scopus Author ID: 36967419300
Russian Federation
Doctor of Biological Sciences, Professor, Lead of the Department of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev", tel. 89202817861, 95 Komsomolskaya st., Orel, Russia. E-mail: gladnova@yandex.ru other IDs: Scopus Author ID:6602758422, Scopus Author ID: 36967419300
Inna E. Fedotova
Orel State University named after I.S. Turgenev
Russian Federation
Candidate of Agricultural Sciences, Associate Professor, Lead of the Sub-department of Soil Science and Applied Biology FSBEI of Higher Education "Orel State University named after I.S. Turgenev ", Orel, Russia.
Russian Federation
Candidate of Agricultural Sciences, Associate Professor, Lead of the Sub-department of Soil Science and Applied Biology FSBEI of Higher Education "Orel State University named after I.S. Turgenev ", Orel, Russia.
Margarita G. Kurochickaya
Orel State University named after I.S. Turgenev
Russian Federation
Candidate of Biological Sciences, Associate Professor at the Sub-department of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev ", Orel, Russia.
Russian Federation
Candidate of Biological Sciences, Associate Professor at the Sub-department of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev ", Orel, Russia.
Valentina V. Silyutina
Orel State University named after I.S. Turgenev
Russian Federation
Assistant at the Subdepartment of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev", Orel, Russia.
Russian Federation
Assistant at the Subdepartment of Ecology and General Biology, FSBEI of Higher Education "Orel State University named after I.S. Turgenev", Orel, Russia.
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Review
For citations:
Ladnova G.G., Fedotova I.E., Kurochickaya M.G., Silyutina V.V. CYTOGENETIC CHANGES IN CELLS OF THE APICAL MERISTEM OF GREEN PLANTING OF THE CITY DEPENDING ON THE LEVEL OF ANTHROPOGENIC LOAD. South of Russia: ecology, development. 2017;12(3):146-152. (In Russ.) https://doi.org/10.18470/1992-1098-2017-3-146-152
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