THE ROLE OF WILD BIRDS IN PRESERVATION AND PREVALENCE OF AVIAN PARAMYXOVIRUS SEROTYPE 1 (NEWCASTLE DISEASE VIRUSES) IN SIBERIA AND THE FAR EAST, RUSSIA

The aim is to evaluate ecological diversity of wild birds in Siberia and the Russian Far East, which are carriers of Newcastle disease virus that belongs to potentially dangerous pathogen for poultry.

Methods. Biological materials (cloacal swabs and intestinal fragments) of wild migratory birds were collected in 2008-2014. The viral isolates were propagated in the allantoic cavity of developing chicken embryos. The presence of virus was determined in hemagglutination tests and primary identification of Newcastle disease virus was confirmed by RT-PCR. Pathogenicity of the obtained isolates was determined in tests ICPI and MDT.

Results. 4443 samples were obtained from wild migratory birds of 11 avian orders and were investigated. Newcastle disease virus was detected in 40 birds from 4 orders. The Duck family (Anatidae) of the Waterfowl order (Anseriformes) plays the leading role in the circulation of Newcastle disease virus in Siberia and the Far East.The main species among them - a teal (Anas crecca), a garganey (Anas querquedula), a mallard (Anas platyrhynchos) and a shoveler (Anas clypeata). All studied isolatesof Newcastle disease virus are apatogenic except for two deponated strains.

Main conclusions. Wild migratory birds from orders Anseriformes and Charadriiformes are capable of carrying Newcastle diseasevirus and could transfer pathogenic variants of this virus to the Russian territory.

1. Mayo M.A. A summary of taxonomic changes recently approved by ICTV. Arch. Virol. 2002. Vol.147. P.1655–1633. doi: 10.1007/s007050200039

2. Alexander D.J., Senne D.A. Newcastle disease, other avian paramyxoviruses, and pneumovirus infections. In Saif Y.M., Barnes H.J., Glisson J.R., Fadly A.M., Mc Dougald L.R., Swayne D.E. (Eds.). Diseases of Poultry 12th edn - Iowa, USA: Blackwell Publishing, 2008. P.74-115.

3. Alexander D.J. Newcastle disease and other avian Paramyxoviridae infections. Diseases of poultry. Ed. by B. W. Calneck. Ames; IA. 1997. P. 541– 569.

4. Thomas N.J., Hunter D.B., Atkinson C.T. Infectious diseases of wild birds. 1st edn. Iowa, USA: Wiley-Blackwell Publishing. 2007. p. 496.

5. Grimes S.E. A Basic Laboratory Manual for the Small Scale Production and Testing of I-2 Newcastle Disease Vaccine. Food and Agricultural Organization (FAO), Animal Production and Health Commission for Asia and the Pacific (APHCA). 2002.

6. Miller P.J., Decanini E.L., Afonso C.L. Newcastledisease: Evolution of genotypes and the related diagnostic challenges. Infect. Genet. Evol. 2010. Vol. 10. (1). P. 26-35. doi: 10.1016/j.meegid.2009.09.012

7. OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 7th ed. Paris, France: Office International des Epizooties, 2012. p.19.

8. Boere G.C., Stroud D.A. The flyway concept: what it is and what it isn’t. In: G.C. Boere, C.A. Galbraith & D.A. Stroud (eds). Waterbirds around the world. The Stationery Office, Edinburgh, UK. 2006. P. 40-47.

9. The National Training Course on Animal Influenza Diagnosis and Surveillance. 2001. Text. Harbin, China (May 20–26, 2001). Harbin. P. 79.

10. Mia Kim L., Suarez D.L., Afonso C.L. Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. J. Vet Diagn Invest. 2008. Vol. 20(4). P. 414–425.

11. Miller P.J. Decanini E.L., Afonso C.L. Newcastle disease: evolution of genotypes and the related diagnostic challenges. Infect. Genet. Evol. 2010. Vol. 10 (1). P. 26-35.

12. Yurchenko K.S., Sivay M.V., Glushchenko A.V., Alkhovsky S.V., Shchetinin A.M., Shchelkanov M.Y., Shestopalov A.M. Complete Genome Sequence of a Newcastle Disease Virus Isolated from a Rock Dove (Columba livia) in the Russian Federation. Genome Announ. 2015. Vol. 3(1). pii: e01514-14. doi:10.1128/genomeA.01514-14. Available at: https://www.researchgate.net/journal/2169-8287_Genome_Announcements). (accessed 27.12.2015)

13. Yurchenko K.S., Sobolev I.A., Glushchenko A.V., Shestopalov A.M. Complete Genome Sequence of Genotype Ib Newcastle Disease Virus Isolated from a Mallard (Anas platyrhynchos) in Russia. Genome Announ. 2015. Vol. 3(6). pii: e01414-15. doi: 10.1128/genomeA.01414-15. Available at: https://www.researchgate.net/journal/2169-8287_Genome_Announcements). (accessed 27.12.2015)

14. Galbraith C.A., Jones T., Kirby J., Mundkur T. A. Review of Migratory Bird Flyways and Priorities for Management. Bonn, Germany: UNEP/CMS Secretariat. 2014. 164 p.

15. Veen, J., AS.K. Yurlov, S. Delany, A.I. Mihantiev, M.A. Selivanova & G.C. Boere. An atlas of movements of Southwest Siberian waterbirds. Wetlands International, Wageningen, The Netherlands. 2005. 60 p.

16. Official site of OIE - World Organisation for Animal Health. Available at: http://www.oie.int/. (accessed 27.12.2015)