Highly hydroxylated fullerenols С₆₀(OH) ₃₆ as potential environmental pollutants: Evaluation of antioxidant activity in liver

The aim of this work was to study the antioxidant properties of fullerenol C60(OH)36 in various in vitro model systems, as well as to evaluate the protective effectiveness of fullerenol against proteins and lipids of liver homogenates.
The experiments were carried out on liver homogenates of white
laboratory rats. At the initial stage, the antioxidant activity of fullerenol in various concentrations (from 1×10‐5 to 0.2 mg/ml) was evaluated. Since the fullerenol concentration of 0.001 mg/ml showed a sufficiently high antioxidant activity in the epinephrine autoxidation system, we used it to study the antioxidant activity in other model systems in which artificial induction of oxidative stress was performed.
Subsequently, control values were obtained in each model system, relative to which the effectiveness of fullerenol as an antioxidant was judged. The protective efficacy of fullerenol against liver homogenate proteins and lipids was evaluated. The effect of fullerenol on the activity of the superoxide dismutase enzyme was also studied. Using the model system, it was shown that fullerenol prevents oxidative damage to lipids.
A study of the intensity of oxidative modification of proteins in the Fe+2/H2O2 model system (Fenton's medium) showed that fullerenol effectively reduced the increase in carbonyl groups. C60(OH)36 at a concentration of 0.001 mg/ml reduced the rate of ROS generation in rat liver mitochondria, which resulted in a decrease in the fluorescence intensity of the ROS‐sensitive (reactive oxygen species) the probe.

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