Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia
https://doi.org/10.18470/1992-1098-2025-2-1
Abstract
Aim. To study some biological properties of the obtained strain of Newcastle disease virus NDV/Adygea/duck/12/2008, including the degree of virulence, and to conduct a phylogenetic study.
Biological material from wild migratory birds was collected in 2008 during the hunting season. Isolation and cultivation of the isolated strains were carried out in the system of developing chicken embryos (RCE). Primary identification confirming the presence of a hemagglutinating agent in the allantoic fluid was carried out in the hemagglutination reaction (HR). Pathogenicity was assessed by MDT and ICPI methods. Sequencing, phylogenetic analysis, the genotype of the studied strain was determined.
The results of studying the main biological properties of the Newcastle disease virus strain NDV/Adygea/duck/12/2008, isolated from wild migratory birds in the Southern Federal District, are presented. According to the phylogenetic study, the NDV/Adygea/duck/12/2008 strain belongs to genotype VII and genetic class 2. The MDT and ICPI virulence tests, as well as the molecular genetic study, classified the described strain as highly pathogenic.
Keywords
Newcastle disease virus,
avian paramyxoviruses,
wild migratory birds,
velogenic strain,
genotype VII
Supporting agencies: The work was supported by the Russian Science Foundation grant, Creation of an Up-to-Date Collection of Modern Newcastle Disease Viruses (NDV) for the Development of Immunological Biological Products, RSF № 24-24-00367.
About the Authors
A. V. Glushchenko
Federal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
Alexandra V. Glushchenko, Researcher, Laboratory of Experimental Oncology and Pharmaceutical Testing, Research Institute of Virology
2 Timakova St, Novosibirsk, Russia 630060.
Tel. +79134696677
K. S. Yurchenko
Federal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
Kseniya S. Yurchenko
Novosibirsk
L. S. Adamenko
Federal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
Lyubov S. Adamenko
Novosibirsk
Yu. I. Karkavin
Federal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
Yuriy I. Karkavin
Novosibirsk
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Review
For citations:
Glushchenko A.V., Yurchenko K.S., Adamenko L.S., Karkavin Yu.I. Molecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russia. South of Russia: ecology, development. 2025;20(2):6-13. (In Russ.) https://doi.org/10.18470/1992-1098-2025-2-1
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