Morphological features and anatomical structure of the leaves of Dagestan endemic species of Allium

   Aim. To conduct a comparative analysis and assessment of the taxon specificity of the morphological and anatomical features of the leaves of endemic species of the genus Allium in Dagestan.

   Material and Methods. The research materials were samples of endemic species of the genus Allium from the natural populations of mountainous Dagestan: A. daghestanicum; A. gunibicum; A. charadzeae; A. mirzojevii and A. samurense. For the analysis of morphometric features, 10 generative individuals were collected in the full flowering phase. Processing of the collected material was carried out at the Laboratory of Introduction and Genetic Resources of the Mountain Botanical Garden, Dagestan Federal Research Centre, Russian Academy of Sciences. Fixation of the material and preparation of temporary micropreparations were carried out according to the generally accepted methods of anatomical studies. Anatomical studies were carried out on leaves of medium formation from the middle part of the leaf. Morphometric parameters of tissues and cells were measured using a Levenhuk D870T optical microscope with an eyepiece micrometer. Micropreparations were photographed using a Lomo‐AT 054 optical microscope and a DCM 510 SCOP video eyepiece.

   Results. A grouping of species according to leaf size was carried out: large‐leaved (A. daghestanicum, A. gunibicum, A charadze section Dagestanica. Tscholok. Friesen) and small‐leaved (A. mirzojevii, A. samurense section Oreiprason F. Herm.), according to the shape of the leaf cross section: grooved with smooth edges (A. daghestanicum), grooved with ribbed edges (A. gunibicum and A charadze) and rounded ribbed (A. mirzojevii and A. samurense), according to the number and size of vascular bundles. Descriptions of the anatomical structure of the leaves and an assessment of the species specificity of the studied traits of five species of Allium were carried out.

   Conclusions. It was determined that some features of the morphology and anatomy of the leaves of five endemic species of the genus Allium are species‐specific or due to environmental conditions of growth in the arid conditions of Inner Mountain Dagestan.

1. Cheremushkina V.A. Biologiya lukov Evrazii [Biology of Eurasian onions]. Novosibirsk, The Science Publ., 2004, 276 p. (In Russian)

2. Kudryashova G.L. Review of species of the genus Allium (Alliaceae) of the Caucasus. Botanicheskii zhurnal [Botanical Journal]. 2001, vol. 86, no. 4, pp. 119–132. (In Russian)

3. Murtazaliev R.A. Konspekt flory Dagestana [Synopsis of the flora of Dagestan]. Makhachkala, Epokha Publ., 2009, vol. 4, pp. 37–45. (In Russian)

4. Krasnaya kniga Respubliki Dagestan [Red Book of the Republic of Dagestan]. Makhachkala, Printing house IE Jamaludinov M.A. Publ., 2020, 800 p. (In Russian)

5. Cholokashvili N.B. A new series of Daghestanica Tscholokaschvili from the section Rhiziridum of the genus Allium L. Zametki sistematiki geografii rastenii [Notes on the taxonomy and geography of plants]. 1965, iss. 25, pp. 83–102. (In Russian)

6. Alibegova A.N., Murtazaliev R.A. On the species independence of Allium mirzajevii Tscholok. (Alliaceae). Novosti sistematiki vysshih rastenii [News of taxonomy of higher plants]. 2010, vol. 41, pp. 63–68. (In Russian)

7. Murtazaliev R.A., Frizen N.V. Biogeografiya i filogeniya vidov sekcii Daghestanica (Allium Amaryllidaceae) [Biogeography and phylogeny of species of the section Daghestanica (Allium Amaryllidaceae)]. In: Trudy 14 s´ezda Russkogo botanicheskogo obshhestva i konferencii «Botanika v sovremennom mire», Mahachkala, 18–23 iyunya 2018. [Proceedings of the 14^th Congress of the Russian Botanical Society and the Conference "Botany in the Modern World", Makhachkala, 18–23 June 2018]. Makhachkala, 2018, vol. 1, pp. 144–147. (In Russian)

8. Alibegova A.N., Musaev A.M. Interpopulation variability in size and weight of seeds of some species of Allium L. (Alliaceae) under conditions of introduction. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Geografiya. Geoekologiya [Bulletin of the Voronezh State University. Series: Geography. Geoecology]. 2011, no. 2, pp. 126–128. (In Russian)

9. Dibirov M.D. Analysis of the variability of seed productivity of Allium samurense Tschlok. in mountain conditions. Problems of development of the agro‐industrial complex of the region. 2020, no. 4(44), pp. 46–52. (In Russian) URL: https://apk05.ru/documents/apk2020/apk_4_2020_ann.pdf

10. Dibirov M.D. Analysis of seed productivity of Allium daghestanicum in the mountains. Proceedings of the Orenburg State Agrarian University, 2021, no. 6(92), pp. 110–114. (In Russian) URL: https://cyberleninka.ru/article/n/analiz-semennoy-produktivnosti-allium-daghestanicum-v-gornyh-usloviyah?ysclid=lr62galm71494585531

11. Dibirov M.D. Variability of morphological features of Allium gunibicum Miscz. ex Grossh. in experimental conditions. Proceedings of the Gorsky Agrarian University, 2022, vol. 59(4), pp. 224–231. (In Russian) doi: 10.54258/20701047_2022_59_4_224

12. Dibirov M., Alibegova A., Osmanov R. The structure of variability of traits of seed productivity of Allium charadzeae Tschlok. in the mountainous conditions of Dagestan. BIO Web of Conferences 43, 2022, article id: 01018. doi: 10.1051/bioconf/20224301018

13. Yamalov S.M., Lebedeva M.V., Golovanov Ya.M., Petrova M.V. Rare and in need of protection plant species of the stony steppes of the Southern and Middle Urals. Samara Scientific Bulletin, 2019, vol. 8, no. 4(29), pp. 107–115. (In Russian) doi: 10.17816/snv201984119

14. Barykina R.P., Veselova T.D., Devyatov A.G., Dzhalilova Kh.Kh., Ilyina G.M., Chubatova N.V. Spravochnik po botanicheskoi mikrotehnike. Osnovy i metody [Handbook of botanical microtechnology. Fundamentals and methods]. Moscow, MSU Publ., 2004, 312 p. (In Russian)

15. Samylina I.A., Anosova O.G. Farmakognoziya. Atlas [Pharmacognosy. Atlas]. Moscow, GEOTAR – Media Publ., 2007, vol. 1, 192 p. (In Russian)

16. Mashenkov M.I., Garipova R.F. Morphophysiological variability of the traits of different varieties of onion ‐ A. sepa, induced by the environmental conditions of the Orenburg region. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta [Proceedings of the Orenburg State Agrarian University]. 2004, vol. 2, no. 2, pp. 97–98. (In Russian)