Avian paramyxovirus 4 isolated from the mallard (Anas platyrhynchos, Linnaeus, 1758): the first case detected in the Western Caspian region
A. A. Derko,
N. A. Dubovitskiy,
T. A. Murashkina,
I. A. Sobolev,
M. V. Solomatina,
A. Yu. Alekseev,
M. G. Magomedov,
J. Mine,
Yu. Uchida,
T. Saito,
M. M. Kallaeva,
K. A. Sharshov https://doi.org/10.18470/1992-1098-2021-3-81-87
Abstract
Aim. The work is aimed at exploring the molecular and genetic properties of avian paramyxovirus 4 (APMV‐4) isolated from the mallard duck.
Material and Methods. In 2018, as a part of an influenza A virus surveillance programme, biological samples were collected from wild birds on Lake Adzhi (Papas) in the Republic of Dagestan. The isolation of virus strains from biological samples was performed using 10‐day‐old special pathogen‐free chicken eggs. The primary detection of hemagglutinating agents in the fluid of the allantoic cavity was carried out via hemagglutination assay. The presence of RNA of the influenza A virus was determined using Real Time PCR. Then, for samples negative in PCR for the influenza A virus, the complete genome DNA sequences were established using NGS sequencing. It was determined that the APMV‐4 strain obtained belonged to intraspecific genetic lines was determined using phylogenetic analysis.
Results. APMV‐4 strain was isolated for the first time in the Western Caspian region. Phylogenetic analysis of the complete genome and gene F showed that the APMV‐4/mallard/Dagestan/92d/2018 strain belongs to the Eurasian clade of subgenotypes. Analysis of amino acid substitutions showed that the cleavage site of the fusion protein is monobasic which is characteristic of lentogenic strains.
Conclusion. During the surveillance program for influenza A viruses in autumn 2018, the APMV‐4 strain was isolated from the mallard duck. Analysis revealed that the APMV‐4 strain was closely phylogenetically related to strains isolated from two wild and one domestic duck from China. This suggests an interconnection between the bird populations in China and the Western Caspian region within the West Asian‐East African migration route.
Supporting agencies: This work was supported by grants from the Russian Science Foundation RSF 20‐44‐07001 "Distribution of RNA viruses of birds in North Asia and the Asia‐Pacific region: genetic diversity, pathogenic potential and prediction of the impact on poultry farming" (laboratory diagnostics, virological experiments, material collection, analysis) and the Russian Foundation for Basic Research RFBR 21‐54‐53031 "Comparative analysis of the diversity of the intestinal microbiome and virome in waterfowl of the Central Asian region (Qinghai plateau and the south of Western Siberia)" (analysis). It was also partially supported by project JPJ008837 "Commissioned projects for promotion of strategic international collaborative research" funded by the Ministry of Agriculture, Forestry and Fisheries of Japan.
About the Authors
A. A. Derko
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Anastasiya A. Derko, Junior Researcher
2 Timakova St, Novosibirsk, 630117
Tel. +79232547996
N. A. Dubovitskiy
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Nikita A. Dubovitskiy
Novosibirsk
T. A. Murashkina
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Tatyana А. Murashkina
Novosibirsk
I. A. Sobolev
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Ivan A. Sobolev
Novosibirsk
M. V. Solomatina
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Maria V. Solomatina
Novosibirsk
A. Yu. Alekseev
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Alexander Yu. Alekseev
Novosibirsk
M. G. Magomedov
Dagestan State Medical University
Russian Federation
Russian Federation
Magomed G. Magomedov
Makhachkala
J. Mine
National Institute of Animal Health
Japan
Japan
Junki Mine
Tsukuba
Yu. Uchida
National Institute of Animal Health
Japan
Japan
Yuko Uchida
Tsukuba
T. Saito
National Institute of Animal Health
Japan
Japan
Takehiko Saito
Tsukuba
M. M. Kallaeva
Dagestan State Medical University
Russian Federation
Russian Federation
Mariam M. Kallaeva
Makhachkala
K. A. Sharshov
Federal Research Centre of Fundamental and Translational Medicine
Russian Federation
Russian Federation
Kirill A. Sharshov
Novosibirsk
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Review
For citations:
Derko A.A., Dubovitskiy N.A., Murashkina T.A., Sobolev I.A., Solomatina M.V., Alekseev A.Yu., Magomedov M.G., Mine J., Uchida Yu., Saito T., Kallaeva M.M., Sharshov K.A. Avian paramyxovirus 4 isolated from the mallard (Anas platyrhynchos, Linnaeus, 1758): the first case detected in the Western Caspian region. South of Russia: ecology, development. 2021;16(3):81‐87. (In Russ.) https://doi.org/10.18470/1992-1098-2021-3-81-87
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