Dynamics of ascorbic acid accumulation in the aboveground phytomass of Galega orientalis Lam.

Aim.To analyse the patterns of ascorbic acid content in the green mass of Galega orientalis (Galega orientalis Lam.), Gale variety, cultivated under introductory conditions to develop an effective method for increasing feed production.

Material and Methods. The influence of the following factors on the accumulation of ascorbic acid in plants was considered: pre‐sowing seed preparation, the presence of a cover crop (pea), cultivation conditions, meteorological factors and the biological age of the grass stand over a three‐year period.

Results. It was established that the plant mass of Galega orientalis is a source of ascorbic acid after plants enter the generative phase of development with predominant localization of vitamins in the leaves (96%). Patterns were revealed of ascorbic acid accumulation in the green phytomass of Galega orientalis depending on average daily temperature during the vegetation period (r = ‐0.69) and specific leaf surface (r = ‐0.83‐0.88).

Conclusion. The data obtained also indicate a statistically significant positive effect of using Baikal‐EM1 microbiological fertilizer for seed inoculation during preparation for sowing, and a negative effect of the cover crop on the accumulation of ascorbic acid in the plant mass of Galega orientalis in its virginal and generative periods of development. 

1. Vagunin D.A., Kapsamun A.D., Ivanova N.N. Agrophytocenosis Galega orientalis Hay use based on new promising varieties. International Scientific Journal, 2016, part 5, no. 6 (48), pp. 165‐167. (In Russian) DOI: 10.18454 / IRJ.2016.48.091

2. Shaikova T.V., Baeva V.S. Technological features of the cultivation of Galega orientalis perspective species selection of the Pskov Research Institute of Agriculture. AgroEkoInzheneriya [AgroEcoEngineering]. 2017, iss. 93, pp. 89‐94. (In Russian)

3. Moiseeva E.A., Bordey R.Kh. Ecological and biological assessment of eastern galega (Galega Orientalis Lam.) as a prospective crop for introduction under the conditions of the city of Surgut. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta [Bulletin of the Krasnoyarsk State Agrarian University]. 2017, no. 10, pp. 140‐147. (In Russian)

4. Mikhailova I.V., Akhtulova E.M. Prospects for cultivating eastern galega (Galega Orientalis Lam.) in the Kola peninsula. Agrokhimiya [Agrochemistry]. 2013, no. 7, pp. 49‐55. (In Russian)

5. Wang J., Huang R. Modulation of ethylene and ascorbic acid on reactive oxygen species scavenging in plant salt response. Frontiers in plant science, 2019, vol. 10, no. 319. DOI: 10.3389/fpls.2019.00319

6. Kumar U., Kaviraj M., Panneerselvam P., Priya H., Chakraborty K., Swain P., Chatterjee S.N., Sharma S.G., Nayak P.K., Nayak A.K. Ascorbic acid formulation for survivability and diazotrophic efficacy of azotobacter chroococcum Avi2 (MCC 3432) under hydrogen peroxide stress and its role in plant‐growth promotion in rice (Oryza sativa L.). Plant physiology and biochemistry, 2019, vol. 139, pp. 419‐427. DOI: 10.1016/j.plaphy.2019.04.003

7. Xing C.H., Liu Y., Zhao L.Y., Zhang S.L., Huang X.S. A novelMYB transcription factor regulates ascorbic acid synthesis and affects cold tolerance. Plant, cell and environment, 2019, vol. 42, iss. 3, pp. 832‐845. DOI: 10.1111/pce.13387

8. Janmohammadi M., Sabaghnia N., Mahfoozi S. Frost tolerance and metabolite changes of rye (Secale cereale) during the cold hardening and overwintering. Acta physiologiae plantarum, 2018, vol. 40, iss. 3, article number 42. DOI: 10.1007/s11738‐018‐2620‐0

9. Alamri S.A., Siddiqui M.H., Al‐Khaishany M.YY., Khan M.N., Ali H.M., Alaraidh I.A., Alsahli A.A., Al‐Rabiah H., Mateen M. Ascorbic acid improves the tolerance of wheat plants to lead toxicity. Journal of plant interactions, 2018, vol. 13, iss. 1, pp. 409‐419. DOI: 10.1080/17429145.2018.1491067

10. Heidari‐Zefreh A.A., Shariatpanahi M.E., Mousavi A., Kalatejari S. Enhancement of microspore embryogenesis induction and plantlet regeneration of sweet pepper (Capsicum annuum L.) using putrescine and ascorbic acid. Protoplasma, 2019, vol. 256, iss. 1, pp. 13‐24. DOI: 10.1007/s00709‐018‐1268‐3

11. Barchuk O.Z., Lysiuk R.M., Denys A.I., Zaliska O.M., Smalyuh O.G., Nester M.I. Experimental study of goat’s rue (Galega Officinalis L.) herb and its liquid extracts. The Pharma Innovation Journal, 2017, vol. 6, iss. 11, pp. 393‐397. Available at: https://www.researchgate.net/publication/321168035_Experimental_study_of_goat's_rue_Galega_Officinalis_L_herb_and_its_liquid_extracts (accessed 16.12.2019)

12. Darmohray L.M., Sedilo G.M., Gutyj B.V. Conceptual framework for the assessment of the nutritional and biological value of the plant Galega orientalis (LAM). Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 2017, vol. 19. no. 79, pp. 9‐12. DOI: 10.15421/nvlvet7902

13. Akram N.A., Shafiq F., Ashraf M. Ascorbic acid‐A potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Frontiers in plant science, 2017, vol. 8, no. 613. DOI: 10.3389/fpls.2017.00613

14. Dospekhov B.A. Metodika polevogo opyta (s osnovami statisticheskoi obrabotki rezul'tatov issledovanii) [Methodology of field experience (with the basics of statistical processing of research results)]. Moscow, Book on Demand Publ., 2012, 352 p. (In Russian)

15. Hewitt E.J., Dickes G.J. Spectrophotometric measurements on ascorbic acid and their use for the estimation of ascorbic acid and dehydroascorbic acid in plant tissues. Biochemical Journal, 1961, vol. 78, no. 2, pp. 384‐391. DOI: 10.1042/bj0780384

16. Chupakhina G.N. Fiziologicheskie i biokhimicheskie metody analiza rastenii [Physiological and biochemical methods of plant analysis]. Kaliningrad, 2000, 59 p. (In Russian)

17. Ovcharov K.E. Vitaminy rastenii [Vitamins of plants]. Moscow, Kolos Publ., 1969, 328 p. (In Russian)

18. Egorov A.D. Vitamin “S” i karotin v rastitel'nosti Yakutii [Vitamin "C" and carotene in the vegetation of Yakutia]. Moscow, USSR Academy of Sciences Publ., 1954, 248 p. (In Russian)

19. Chitanova G.V. Dynamics of the content of ascorbic acid in eucalyptus leaves. Trudy Suhumskogo Botanicheskogo sada [Works of the Sukhumi Botanical Garden]. 1982, no. 27, p. 49‐56. (In Russian)