Species of Odontia and Tomentella (Thelephorales, Basidiomycota) new to Dagestan, Russia
https://doi.org/10.18470/1992-1098-2020-3-165-173
Abstract
Aim. To obtain new data on the species diversity, phylogenetic structure, and ecological characteristics of thelephoroid fungi (Thelephorales, Basidiomycota) in the Republic of Dagestan.
Material and Methods. Both micromorphological and molecular analyses were used for studying of the fungal specimens collected by the authors in 2018-2019 in the Gunibsky and Magaramkentsky Districts of Dagestan. Additional specimens from the Mycological Herbarium of the Komarov Botanical Institute of the Russian Academy of Sciences (LE) were studied. The ITS region of nrDNA was amplified with two pairs of primers, ITS1F/ITS4 and ITS5/ITS4.
Results. Sixteen ITS sequences belonging to eight species were obtained from the studied material. Of them, 14 sequences clustered in the Tomentella clade and two sequences nested within the Odontia clade. Four species – Odontia duemmeri, Tomentella lapida, T. radiosa, T. terrestris – were registered for the first time for Dagestan. Detailed information on the specimens studied is presented. Species identification of Odontia fibrosa, Tomentella badia, T. ferruginea, and T. stuposa was confirmed by ITS nrDNA analysis.
Conclusion. Data on the species richness of the genera Odontia and Tomentella in Dagestan is updated, and the species T. lilacinogrisea is excluded from the regional funga. To date the genera Odontia and Tomentella in the Republic of Dagestan are represented by three and fifteen species, respectively.
Keywords
Biodiversity,
basidiomycetes,
distribution of fungi,
ITS phylogeny,
DNA barcodes,
Tomentella,
Dagestan,
Caucasus
Supporting agencies: This study was funded by the RFBR according to research project No19‐34‐50111. The research was done using the equipment of the Core Facility Centre, Cell and Molecular Technologies in Plant Science at the Komarov Botanical Institute, Russian Academy of Sciences (St‐Petersburg, Russia). Morphological studies of LE specimens were carried out within the framework of the research project of the Koma‐ rov Botanical Institute RAS, Herbarium Funds of the BIN RAS (History, Conservation, Study and Supplementation) (АААА‐А18‐118022090078‐2).
About the Authors
Yu. Yu. Ivanushenko
Dagestan State University
Russian Federation
Russian Federation
Yuliya Yu. Ivanushenko
Makhachkala
S. V. Volobuev
Komarov Botanical Institute, Russian Academy of Sciences
Russian Federation
Russian Federation
Sergey V. Volobuev, Candidate of Sciences (Biology), Senior Researcher, Laboratory of Systematics and Geography of Fungi
2 Professora Popova St, Saint Petersburg, 197376
Tel. +7(812)3725469
References
1. Ivanushenko Yu.Yu. [The history of the study of afilloforoid fungi in the Republic of Dagestan]. In: Universitetskaya ekologiya [University Ecology]. Makhachkala, IAE Publ., 2019, pp. 16-18. (In Russian)
2. Bagdasarova A.F. [Fungi of liana forest in the Samur River Delta]. In: Botanika, fiziologiya rasteniy i rastenievodtsva [Botany, physiology of plants and plant growing]. Makhachkala, 1965, pp. 64-70. (In Russian)
3. Ghobad-Nejhad M., Hallenberg N., Parmasto E., Kotiranta H. A first annotated checklist of corticioid and polypore basidiomycetes of the Caucasus region. Mycologia Balcanica, 2009, vol. 6, iss. 3, pp. 123-168. DOI: 10.5281/zenodo.2550071
4. Viner I.A. [New records of polypores and corticioid fungi in Dagestan]. In: Trudy gosudarstvennogo prirodnogo zapovednika “Dagestanskii” [Proceedings of "Dagestanskiy" State Nature Reserve]. Makhachkala, 2017, vol. 13, pp. 13-19. (In Russian)
5. Volobuev S.V., Ivanushenko Yu.Yu., Ismailov A.B. Auriporia aurulenta – kandidat v Krasnuyu knigu Respubliki Dagestan [Auriporia aurulenta – a proposal to the Red Data Book of the Republic of Dagestan]. Materialy Mezhdunarodnoi konferentsii «Flora i zapovednoe delo na Kavkaze: istoriya i sovremennoe sostoyanie izuchennosti», Pyatigorsk, 2019 [Materials of the International Conference "Flora and reserved matter in the Caucasus: history and current state of knowledge", Pyatigorsk, 2019]. Pyatigorsk, 2019, pp. 32-33. (In Russian)
6. Ivanushenko Yu.Yu., Ismailov A.B., Volobuev S.V. Pervye svedeniya o trutovykh gribakh plato Gunib (Respublika Dagestan) [First data on polypores of the Gunib Plateau (the Republic of Dagestan)]. Materialy XXI Mezhdunarodnoi nauchnoi konferentsii “Biologicheskoe raznoobrazie Kavkaza i yuga Rossii”, Magas, 2019 [Materials of the XXI International Scientific Conference "Biological Diversity of the Caucasus and the South of Russia", Magas, 2019]. Magas, 2019, pp. 163-166. (In Russian)
7. Volobuev S.V., Ivanushenko Yu.Yu. Aphyllophoroid fungi (Basidiomycota) on juniper on the Gunib Plateau, inner-mountain Dagestan. Czech Mycology, 2020, vol. 72, iss. 1, pp. 83-93. DOI: 10.33585/cmy.72106
8. Volobuev S.V. Aphyllophoroid fungi of the "Samurskiy" national park (Dagestan). Mikologiya i fitopatologiya, 2020, vol. 54, no. 4, pp. 235-243. DOI: 10.31857/S002636482004011X
9. Volobuev S.V., Ivanushenko Y.Yu., Ismailov A.B. New for Dagestan species of Tomentella (Thelephorales, Basidiomycota). South of Russia: ecology, development, 2019, vol. 14, no. 2, pp. 172-179. (In Russian) DOI: 10.18470/1992-10982019-2-172-179
10. Kõljalg U. Tomentella (Basidiomycota) and related genera in temperate Eurasia. Fungiflora, Oslo, 1996.
11. White T.J., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. San Diego, Academic Press, 1990, pp. 315-322.
12. Gardes M., Bruns T.D. ITS primers with enhanced specificity for Basidiomycetes: application to the identification of mycorrhizae and rusts, Mol Ecol., 1993, vol. 2, pp. 113-118.
13. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 2013, vol. 30, iss. 12, pp. 2725-2729. DOI: 10.1093/molbev/mst197
14. GenBank. Available at: www.ncbi.nlm.nih.gov/genbank/ (accessed 20.04.2020)
15. UNITE. Available at: https://unite.ut.ee/index.php (accessed 20.04.2020)
16. Katoh K., Rozewicki J., Yamada K.D. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 2019, vol. 20, pp. 1160-1166. DOI: 10.1093/bib/bbx108
17. MAFFT version 7. Available at: http://mafft.cbrc.jp/alignment/server/ (accessed 20.04.2020)
18. Trifinopoulos J., Nguyen L.T., von Haeseler A., Minh B.Q. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 2016, vol. 44, iss. W1, pp. W232-W235. DOI: 10.1093/nar/gkw256
19. Tedersoo L., Harend H., Buegger F., Pritsch K., Saar I., Kõljalg U. Stable isotope analysis, field observations and synthesis experiments suggest that Odontia is a nonmycorrhizal sister genus of Tomentella and Thelephora. Fungal Ecology, 2014, vol. 11, pp. 80-90. DOI: 10.1016/j.funeco.2014.04.006
20. Martini E. Odontia duemmeri (Wakef.) Kõljalg. Excerpts from Crusts & Jells., iss. 123. pp. 1-8.
21. Zmitrovich I.V., Stolyarskaya M.V., Kalinovskaya N.I., Popov E.S., Myasnikov A.G., Morozova O.V., Volobuev S.V., Bolshakov S.Yu., Svetasheva T.Yu., Bondartseva M.A., Kovalenko A.E. Makromitsety Nizhne-Svirskogo zapovednika (annotirovannyi spisok vidov) [Macromycetes of NizhneSvirsky Reserve (annotated checklist)]. Saint Petersburg, “Svoye izdatel’stvo” Publ., 2015, 185 p. (In Russian)
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For citations:
Ivanushenko Yu.Yu., Volobuev S.V. Species of Odontia and Tomentella (Thelephorales, Basidiomycota) new to Dagestan, Russia. South of Russia: ecology, development. 2020;15(3):165-173. https://doi.org/10.18470/1992-1098-2020-3-165-173
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