Use of Oxidative Stress (MDA) Markers and Cytogenetic Markers in the Ecological‐Genetic Monitoring of the Northern Caspian Sea
https://doi.org/10.18470/1992-1098-2020-1-99-106
Abstract
Aim. Assessment and subsequent long‐term dynamic observation of possible negative genetic consequences of the effect of pollutants on certain units of metabolism are important tasks in ecological‐genetic monitoring. Cytogenetic and biochemical biomarkers are used in biomonitoring studies to analyze the genotoxicity of aquatic pollutants. The purpose of the work was to analyse the use of markers of oxidative stress and cytogenetic disorders in goby fish caught at shallow and deep‐water stations of the Northern Caspian Sea in the ecological‐genetic monitoring system.
Material and Methods. The study was undertaken on 227 specimens of goby fish by cytogenetic and biochemical analysis.
Results. The correlation dependence between erythrocytes with micronuclei and erythrocytes with the quantity of damaged nuclei summarized as R= ‐0.83 (p˂0.05) was shown. The results of correlation analysis between oxidative stress indices and the number of destructive changes in erythrocyte nucleus are presented. Our analysis thus leads us to the conclusion that somatic mutagenesis (micronuclear formation) after exposure to free radicals can be an adaptive response to this stress factor.
Conclusion. Analysis leadsus to the conclusion that somatic mutagenesis (formation of micro‐nuclei) after exposure to free radicals can be an adaptive response to stress factor in habitat conditions in areas of liquidated prospecting wells of LUKOIL‐Nizhnevolzhskneft in the Northern Caspian Sea.
About the Authors
T. V. Kuzina
Astrakhan State University
Russian Federation
Russian Federation
Tatiana V. Kuzina, Cand. Biol. Sci., Senior Researcher
20a (ASU bldg No. 2) Tatishchev St, Astrakan, 414056. Tel. +79033475484
A. V. Kuzin
LUKOIL‐Nizhnevolzhskneft LLC
Russian Federation
Alexey V. Kuzin
Russian Federation
Alexey V. Kuzin
References
1. Genetika okislitel'nogo stressa [Genetics of Oxidizing Stress]. Rostov on Don, SKNC VSh SFY Publ., 2009, 156 p. (In Russian)
2. Dubinina Е.Е. Produkty metabolizma kisloroda v funktsional'noi aktivnosti kletok (zhizn' i smert', sozidanie i razrushenie). Fiziologicheskie i kliniko‐biokhimicheskie aspekty [Products of Oxygen Metabolism in the Functional Activity of Cells (Life and Death, Creation and Destruction). Physiological and Clinical‐Biochemical Aspects]. Saint Petersburg, Medical Press Publ., 2006, 400 p. (In Russian)
3. Zenkov N.K., Lankin V.Z., Menshikova E.B. [Oxidative Stress: Biochemical and Pathophysiological aspects]. Moscow, "Nauka/ Interperiodika" Publ., 2001, 343 p. (In Russian)
4. Baraboy V.A. Mechanism of stress and lipid peroxidation. Uspekhi sovremennoi biologii [Successes of Modern Biology]. 1991, vol. 111, no. 6, pp. 923‐931. (In Russian)
5. Ivanova T.I., Fesenko E.V., Dzikovskaya L.A., Degtyareva E.S., Mkrtchyan L.S., Ovsyannikova N.C., Khorokhorina V.A., Krikunova L.I. A study of chromosome aberrations and markers of oxidative stress (malondialdehyde, plasma total antioxidant activity) among females residing in terri tories contaminated with radionuclides as a result of the Chernobyl accident. Radiation and risk, 2018, vol. 27, no. 2, pp. 46‐61. (In Russian) DOI: 10.21870/0131‐3878‐2018‐27‐2‐46‐61
6. Il'inskikh N.N., Ksents A.S., Il'inskikh E.N., Manskikh V.N., Vasil'ev S.A., Il'inskikh I.N. Mikroyadernyi analiz v otsenke tsitogeneticheskoi nestabil'nosti [Micronuclear analysis in assessing cytogenetic instability]. Tomsk, TSPU Publ., 234 p. (In Russian)
7. Il'inskikh N.N., Novitskii V.V., Vanchugova N.N., Il'inskikh I.N. Mikroyadernyi analiz i tsitogeneticheskaya nestabil'nost' [Micronuclear analysis and cytogenetic in‐stability]. Tomsk, Tomsk University Publ., 1992, 270 p. (In Russian)
8. Mineev A.K. Morphological analysis and pathological changes in the structure of blood cells in fishes from Saratov Reservoir. Voprosy ikhtiologii [Journal of Ichthyology]. 2007, vol. 47, no. 1, pp. 93‐100. (In Russian)
9. Turchenyuk O. V., Tomshina O.L., Kalkov A.P. Micronuclear Test for Environmental Assessment. Omskii nauchnyi vestnik [Omsk Scientific Journal]. 2006, no. 6, pp. 293‐297. (In Russian)
10. Ilyinskikh N.N., Ilyinskikh E.N., Ilyinskikh I.N., Yankovskaya A.E., Saushkin S.A. Hygienic assessment of the state of the environment in the oil and gas‐bearing area based on cytogenetic and molecular genetic methods. Hygiene and sanitation, 2017, vol. 96, no. 2, pp. 121‐124. (In Russian) DOI: 10.18821/0016‐9900‐2017‐96‐2‐121‐124
11. Geraskina S.A., Sarapultseva E.I., eds. Biologicheskii kontrol' okruzhayushchei sredy: geneticheskii monitoring [Biological environmental control: genetic monitoring]. Moscow, Academy Publ., 2010, 208 p. (In Russian)
12. Kuzina T.V., Galaktionova M.L. Analysis of the interrelation of cytogenetic homeostasis and oxidative stress in the organism of goby fish (gobiidae) of the Northern Caspian. South of Russia: ecology, development, 2018, vol. 13, no. 2, pp. 64‐72. (In Russian) DOI: 10.18470/1992‐1098‐2018‐2‐64‐72
13. Stroyev E.A., Makarova V.G. Praktikum po biologicheskoi khimii [Workshop on Biological Chemistry]. Moscow, Vysshaya shkola Publ., 1986, pp. 211‐214. (In Russian)
14. Kozak M.F., Kozak M.V. Biometricheskie metody v nauchnykh issledovaniyakh [Biometric methods in scientific research]. Astrakhan, Astrakhan State University Publ., 2018, 168 p. (In Russian)
15. Shimizu N. Molecular mechanisms of the origin of micronuclei from extrachromosomal elements. Mutagenesis, 2011, vol. 26, iss. 1, pp. 119‐123. DOI: 10.1093/mutage/geq053
16. Trukhachev V.I., Nekrasova I.I., Kvochko A.N. Osnovy obshchei patologii (mestnye reaktsii organizma na povrezhdenie) [Fundamentals of General Pathology (Local Reactions of the Body to Damage)]. Stavropol, AGRUS Publ., 2015, 68 p. (In Russian)
17. Al‐Sabti K., Metcalfe C.D. Fish micronuclei for assessing genotoxicity in water. Mutation Research / Genetic Toxicology, 1995, vol. 343, iss. 2‐3, pp. 121‐135. DOI: 10.1016/0165‐1218(95)90078‐0.
Review
For citations:
Kuzina T.V., Kuzin A.V. Use of Oxidative Stress (MDA) Markers and Cytogenetic Markers in the Ecological‐Genetic Monitoring of the Northern Caspian Sea. South of Russia: ecology, development. 2020;15(1):99-106. (In Russ.) https://doi.org/10.18470/1992-1098-2020-1-99-106
Views:
940
Email this article 
