TESTING OF TWO‐STAGE CULTIVATION OF DUNALIELLA SALINA TEOD. (TEODORESCO, 1905) IN THE SEVASTOPOL REGION
https://doi.org/10.18470/1992-1098-2019-2-211-220
Abstract
Aim. In this work, we set out to study the effect of surface irradiance on the growth rate and the pigment ratio of D. salina, as well as to test a technology for semi‐industrial cultiva‐ tion of D. salina aimed at obtaining its biomass enriched with β‐carotene.
Methods. D. salina was cultivated under semi‐industrial conditions in a greenhouse module of the A. O. Kovalevsky Institute of Marine Biological Research. Square tanks (1 × 1 m) lined with polyethylene film and laid on a flat ground surface were used as propagators. The culture layer had a thickness of 10 cm and a volume of 100 litres. Pre‐grown D. salina was diluted using fresh medium without salts – sources of nitrogen and phosphorus. Dunaliella was cultivated under natural light with continuous stirring.
Results. We determined the range of surface irradiance, which can be considered optimal for intensive cultivation of D. salina in terms of energy and mineral costs. Across this range, the average growth rate of the culture under experimental conditions amounted to 0.23–0.27 g DW/(l∙day), whereas the ratio of Car / Chl a increased by a factor of 1.5‐ 2, which indicates changes in the pigment composition of D. salina. It is experimentally shown that the content of carotenoids in the tanks increased by 1.3 times amounting to 600 mg per 1 m2 at a Car/Chl a ratio of 4.5 at the second stage of D. salina cultivation.
Conclusions. Carotenoid accumulation during semi‐industrial cultivation of D. salina occures due to two factors: in‐ creased natural irradiance and temperature without an additional increase in salinity and blowing of carbon dioxide, which reduces the costs of its industrial cultivation. This two‐stage cultivation method can be used to develop a technology for obtaining Dunaliella biomass enriched with β‐carotene in the southern regions of the Russian Federation.
Keywords
semi‐industrial cultivation of D. salina,
productivity,
Car/Chl a ratio,
greenhouse module,
β‐carotene
Supporting agencies: The studies were conducted under the State Project of IMBR RAS No. 0828‐ 2018‐0004 and the grant No.18‐44‐920009 from the Russian Foundation for Basic Research.
About the Authors
I. N. Gudvilovich
A.O.Kovalevsky Institute of Marine Biological Research of RAS
Russian Federation
Russian Federation
Cand. Sci. (Biol.), Senior Researcher, Department for Biotechnology and Phytoresoursces,
2 Nakhimov ave., Sevastopol, 299011
A. B. Borovkov
A.O. Kovalevsky Institute of Marine Biological Research of RAS
Russian Federation
Russian Federation
Cand. Sci. (Biol.), Head of Department for Biotechnology and Phytoresoursces,
Sevastopol
References
1. Ben‐Amotz A., Polle J.E., Rao D.S. The alga Dunaliella: biodiversity, physiology, genomics and biotechnology. Enfield, NH, Science Publishers, 2009, 555 p.
2. García‐González M., Moreno J., Cañavate J.P., Anguis V., Prieto A., Manzano C., Florencio F.J., Guerrero M.G. Conditions for open‐air outdoor culture of Dunaliella salina in southern Spain. Journal of Applied Phycology, 2003, vol. 15, iss. 2‐3, pp. 177‐184. Doi:10.1023/A:1023892520443
3. Del Campo J.A., García‐González M., Guerrero M.G. Outdoor cultivation of microalgae for carotenoid production: current state and perspectives. Appl. Microbiol. Biotechnol., 2007, vol. 74, iss. 6, pp. 1163‐1174. Doi: 10.1007/s00253‐007‐0844‐9
4. Wu Z., Dejtisakdi W., Kermanee P., Ma C., Arirob W., Sathasivam R., Juntawong N. Outdoor cultivation of Dunaliella salina KU 11 using brine and saline lake water with raceway ponds in northeastern Thailand. Biotechnol. Appl. Biochem., 2017, vol. 64, iss. 6, pp. 938‐943. Doi:10.1002/bab.1537
5. Sathasivam R., Ki J.‐S. A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Mar. Drugs., 2018, vol. 16, iss. 1, 26 p. Doi:10.3390/md16010026
6. Masyuk N.P. Morfologiya, sistematika, ekologiya, geograficheskoe rasprostranenie roda Dunaliella Teod. [Morphology, taxonomy, ecology, geographical distribution of the genus Dunaliella Teod.]. Kiev, Naukova Dumka Publ., 1973, 487 p. (In Russian)
7. Solovchenko A.E., Selivanova E.A., Chekanov K.A., Sidorov R.A., Nemtseva N.V., Lobakova E.S. Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae). Biochemistry (Moscow), 2015, vol. 80, iss. 11, pp. 1508‐1513. Doi: 10.1134/S0006297915110139
8. Borovkov A.B., Gudvilovich I.N. [The testing of two‐stage system of semi‐industrial cultivation of Dunaliella salina Teod.]. Voprosyi sovremennoy algologii, 2017, no. 1 (13). (In Russian) Available at: http://algology.ru/1155 (accessed 16.11.2018).
9. Abdulagatov I.M., Alkhasov A.B., Dogeev G.D., Tumalaev N.R., Aliev R.M., Badavov G.B., Aliev A.M., Salikhova A.S. Technological application of microalgae in power industry and environmental protection. South of Russia: ecology, development, 2018, vol. 13, no. 1, pp. 166‐183. (In Russian) Doi:10.18470/1992‐1098‐2018‐1‐166‐183
10. Borovkov A.B., Gudvilovich I.N. Intensive cultivation of Dunaliella salina for production of biomass with elevated β‐carotene content. Communication 2. Optimization of Cultivation Regime. Hydrobiological Journal, 2015, vol. 51, iss. 4, pp. 31‐38. Doi: 10.1615/HydrobJ.v51.i4.40
11. Xu Y., Ibrahim I.M., Harvey P.J. The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (Chlorophyta) CCAP 19/30. Plant Physiol. Biochem., 2016, vol. 106, pp. 305‐315. Doi: 10.1016/j.plaphy.2016.05.021
12. Minhas A.K., Hodgson P., Barrow C.J., Adholeya A. A Review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids. Frontiers in Micro‐ biology, 2016, vol. 7, art. 546, pp. 1‐19. Doi: 10.3389/fmicb.2016.00546
13. Lamers P.P., Janssen M., De Vos R.C., Bino R.J., Wijffels R.H. Carotenoid and fatty acid metabolism in nitrogen‐starved Dunaliella salina, a unicellular green microalga. J. Biotechnol. 2012, vol. 162, iss. 1, pp. 21‐27. Doi:10.1016/j.jbiotec.2012.04.018
14. Shaish A., Avron M., Ben‐Amotz A. Effect of inhibitors on the formation of stereoisomers in the biosynthesis of β‐carotene in Dunaliella bardawil. Plant. Cell. Physiol., 1990, vol. 31, iss. 5, pp. 689‐696. Doi: 10.1093/oxfordjournals.pcp.a077964
15. Metodyi fiziologo‐biohimicheskogo issledovaniya vodorosley v gidrobiologicheskoy praktike [Methods of physiological and biochemical studies of algae in hydrobiological practice]. Kiev, Naukova Dumka Publ., 1975, 247 p. (In Russian)
16. Wellburn A.R. The spectral determination of chlorophylls a and b, as well as total carote‐ noids, using various solvents with spectrophotometers of different resolution. J. Plant Phys., 1994, vol. 144, iss. 3, pp. 307‐313. Doi:10.1016/S0176‐1617(11)81192‐2
Review
For citations:
Gudvilovich I.N., Borovkov A.B. TESTING OF TWO‐STAGE CULTIVATION OF DUNALIELLA SALINA TEOD. (TEODORESCO, 1905) IN THE SEVASTOPOL REGION. South of Russia: ecology, development. 2019;14(2):211-220. (In Russ.) https://doi.org/10.18470/1992-1098-2019-2-211-220
Views:
816
Email this article 
