EFFECT OF NITROGEN AND HUMIC FERTILIZERS ON THE BIOCHEMICAL STATE OF OIL‐CONTAMINATED CHERNOZEM

Aim. In this paper, we aim to assess the effect of nitrogen and humic fertilizers on the biochemical state of oil‐contaminated chernozem.

Methods. In order to simulate the oil pollu‐ tion, chernozem was exposed to oil doses constituting 1, 5 and 10% of the soil mass for 30, 60 and 90 days. For simulating bioremediation of oil‐contaminated chernozem, the following fertilizers were used: potassium and sodium humates, urea and nitroammophos. Nitrogen fertilizers – urea and nitroammophos having a nitrogen content of 46% and 15%, respectively – were applied to the soil for the purposes of restoring the equilibrium between carbon and nitrogen. Humic fertilizers (potassium and sodium humates) were applied to the soil for stimulating the indigenous oil destructive microbiota. In order to assess the biological activity of the soil, we determined catalase activity, invertase activity, as well as CO2 emission intensity.

Results. The effect of urea, nitroammophos, potassium and sodium humates on the enzymatic activity and CO2 emissions of ordinary chernozem, which had been exposed to various doses of oil (1, 5 and 10% of the soil mass) for 90 days, was studied in a model experiment. Following the introduction of nitroammophos into soil with low levels of oil pollution, catalase activity decreased, whereas respiration and invertase activity increased. Urea introduced into the soil contaminat‐ ed with a 10% dose of oil stimulated catalase activity. At oil concentrations of 1 and 5%, the introduction of potassium and sodium humates had a stimulating effect on enzymic activity and carbon dioxide evolution.

Conclusions. It is advisable to use the intensity of CO2 emissions released from the soil, as well as the invertase activity for diagnosing the state of chernozem con‐ taminated with oil (5‐10%) following the introduction of nitrogen and humic ameliorants. At lower doses of oil, it is advisable to assess the state of the soil following the introduction of nitrogen fertilizers by catalase activity. 

1. Peng R.H., Xiong A.S., Xeo Y., Fu X.Y., Gao F., Zhao W., Tian Y.S., Yao, Q.H. Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Reviews, 2008, vol. 32, pp. 927‐955. Doi: 10.1111/j.1574‐6976.2008.00127.x

2. Kimes N.E., Callaghan A.V., Suflita J.M., Morris P.J. Microbial transformation of the Deepwater Horizon oil spillpast, present, and future perspectives. Frontiers in Microbiology, 2014, no. 5, 603 p. Doi: 10.3389/fmicb.2014.00603

3. Riveroll‐Larios J., Escalante‐Espinosa E., Fócil‐Monterrubio R.L., Díaz‐Ramírez I.J. Biological activity assessment in Mexican tropical soils with different hydrocarbon contamination histories. Water Air Soil Pollution, 2015, vol. 226, iss. 10, 353 p. Doi: 10.1007/s11270‐015‐2621‐1

4. Wolińska A., Kuźniar A., Szafranek‐Nakonieczna A., Jastrzębska N., Roguska E., Stępniewska Z. Biological activity of autochthonic bacterial community in oil contaminated soil. Water Air Soil Pollution, 2016, vol. 227, 130 p. Doi: 10.1007/s11270‐016‐2825‐z

5. Nikolopoulou M., Pasadakis N., Norf H., Kalogerakis, N. Enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids. Marine Pollution Bulletin, 2013, vol. 77, iss. 1‐2, pp. 37‐44. Doi: 10.1016/j.marpolbul.2013.10.038

6. Macaulay B.M., Rees D. Bioremediation of oil spills: a review of challenges for research advancement. Annals of Environmental Science, 2014, vol. 8, pp. 9‐37.

7. Poi G., Shahsavari E., Aburto‐Medina A., Mok P.Ch., Ball A.S. Large scale treatment of total petroleum‐hydrocarbon contaminated groundwater using bioaugmentation. Journal of Environmental Management, 2018, vol. 214, pp. 157‐163. Doi:10.1016/j.jenvman.2018.02.079

8. Polyak Y.M., Bakina L.G., Chugunova M.V., Mayachkina N.V., Gerasimov A.O., Bure V.M. Effect of remediation strategies on biological activity of oil‐contaminated soil ‐ A field study. International Biodeterioration & Biodegradation, 2018, vol. 126, pp. 57‐68. Doi: 10.1016/j.ibiod.2017.10.004

9. Shapovalova N.N., Godunova E.I., Menkina E.A. Influence of fertilizers application aftereffect on ecological and agrochemical state of chernozem ordinary. Agrokhimicheskiy vestnik [Agrochemical Herald]. 2018, no. 5, pp. 9‐15. (In Russian) Doi: 10.24411/0235‐2516‐2018‐10036

10. Varjani S.J., Upasani V.N. A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants. International Biodeterioration & Biodegradation, 2017, vol. 120, pp. 71‐ 83. Doi: 10.1016/j.ibiod.2017.02.006

11. Kireeva N.A., Novoselova E.I., Grigoriadi A.S. Effect of soil pollution by oil on the physiological parameters of plants and the rhizosphere microbiota. Agrokhimiya [Agricultural Chemistry]. 2009, no. 7, pp. 71‐80. (In Russian)

12. Minnikova T.V., Denisova T.V., Kolesnikov S.I., Akimenko Yu.V. Assessment of Agroecological Indicators of Oil‐Contaminated Chernozem in Rostov Oblast after Remediation with Urea and Potassium Humate. Russian Agricultural Sciences, 2018, vol. 44, iss. 2, pp. 177‐180. Doi: 10.3103/S1068367418020118

13. Wu M., Dick W.A., Li W., Wang X., Yang Q., Wang T., Xu L., Zhang M., Chen L., Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum‐contaminated soil. International Biodeterioration & Biodegradation, 2016, vol. 107, pp. 158‐164. Doi: 10.1016/j.ibiod.2015.11.019

14. Gabbasova I.M., Suleymanov R.R., Garipov T.T Degradation and remediation of soils polluted with oil‐field wastewater. Eurasian Soil Science, 2013, vol. 46, no. 2, pp. 204‐211. Doi: 10.1134/S1064229313020051

15. Souza E.C., Vessoni‐Penna T.C., de Souza Oliveira R.P. Biosurfactant‐enhanced hydrocarbon bioremediation: an overview. International Biodeterioration & Biodegradation, 2014, vol. 89, pp. 88‐ 94. Doi: 10.1016/j.ibiod.2014.01.007

16. Dolgova L.G. Oxidoreductase activity as a diagnostic indicator of soil contamination with industrial waste. Pochvovedenie [Eurasian Soil Science], 1978, no. 7, pp. 152‐157. (In Russian)

17. Kolesnikov S.I., Aznaurʹyan D.K., Kazeev K.Sh., Denisova T.V. Studying the Application of Urea and Phosphogypsum as Ameliorants on Oil‐Contaminated Soils in a Model Experiment. Agrokhimiya [Agrochemistry]. 2011, no. 9, pp. 77‐81. (In Russian)

18. Novosyolova E.I., Kireeva N.A. Soil Enzymatic Activity in Conditions of Oil Contamination and its Biodiagnostic Importance. Teoreticheskaya i prikladnaya ekologiya [Theoretical and Applied Ecology]. 2009, no. 2, pp. 4‐12. (In Russian)

19. Minnikova T.V., Denisova T.V., Mandzhieva S.S., Kolesnikov S.I., Minkina T.M., Chaplygin V.A., Burachevskaya M.V., Sushkova S.N., Bauer T.V. Assessing the effect of heavy metals from the Novocherkassk power station emissions on the biological activity of soils in the adjacent areas. Journal of Geochemical Exploration, 2017, vol. 174, pp.70‐78. Doi: 10.1016/j.gexplo.2016.06.007

20. Kochetkov I.A., Kavelenova L.M. Izuchenie dykhatelʹnoi aktivnosti pochv nekotorykh rastitelʹnykh soobshchestv Krasnosamarskogo lesnichestva [The study of the respiratory activity of the soil of some plant communities of Krasnosamarsky’s forestry]. Materialy mezhregionalʹnoi nauchno‐prakticheskoi konferentsii «Ekologicheskie problemy srednego Povolzhʹya», Ulʹyanovsk, 1999 [Materials of the interregional scientific‐practical conference ʺEnvironmental problems of the middle Volga regionʺ, Ulʹyanovsk, 1999]. Ulʹyanovsk, 1999, pp. 128‐130. (In Russian)

21. Kanisʹkin M.A., Terekhova V.A., Yakovlev A.S. Control of humate detoxification of phosphogypsum waste using biotesting methods. Ekologia i promyshlennost Rossii [Ecology and Industry of Russia]. 2007, no. 8, pp. 48‐51. (In Russian)

22. Dindar E., Şağban F.T., Başkaya H.S. Bioremediation of Petroleum‐Contaminated Soil. Journal of Biological and Environmental Sciences, 2013, vol. 7, no. 19, pp. 39‐47.

23. Kireeva N.A., Grigoriadi A.S., Khaibullina E.F. Association of hydrocarbon‐oxidizing microorganisms for bioremediation of oil‐contaminated soils. Vestnik Bashkirskogo universiteta [Bulletin of Bashkir University]. 2009, vol. 14, no. 2, pp. 391‐394. (In Russian)

24. Kolesnikov S.I., Kazeev K.Sh., Tatosyan M.L., Valʹkov V.F. The effect of pollution by oil and oil products on the biological status of ordinary chernozems. Pochvovedenie [Eurasian Soil Science]. 2006, no. 5, pp. 552‐556. (In Russian)

25. Kolesnikov S.I., Kazeev K.Sh., Valʹkov V.F., Aznaurʹyan D.K., Zharkova M.G. Biodiagnostika ekologicheskogo sostoyaniya pochv, zagryaznennykh neftʹyu i nefteproduktami [Biodiagnostics of the ecological condition of soils polluted by oil and oil products]. Rostov‐on‐Done, Rostizdat Publ., 2007, 192 p. (In Russian)

26. Agnello A.C., Bagard M., Van Hullebusch E.D., Esposito G., Huguenot D. Comparative bioremediation of heavy metals and petroleum hydrocarbons co‐contaminated soil by natural attenuation, phytoremediation, bioaugmentation and bioaugmentation‐assisted phytoremediation. Science of the Total Environment, 2016, vol. 563‐564, pp. 693‐703. Doi: 10.1016/j.scitotenv.2015.10.061

27. Baldan E., Basaglia M., Fontana F., Shapleigh J. P., Casella S. Development, assessment and evaluation of a biopile for hydrocarbons soil remediation. International Biodeterioration & Biodegradation, 2015, vol. 98, pp. 66‐72. Doi: 10.1016/j.ibiod.2014.12.002

28. Chen M., Xu P., Zeng G., Yang Ch., Huang D., Zhang J. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs. Biotechnology Advances, 2015, vol. 33, pp. 745‐755. Doi: 10.1016/j.biotechadv.2015.05.003

29. Kazeev K.Sh., Kolesnikov S.I., Akimenko Yu.V., Dadenko E.V. Metody biodiagnostiki nazemnykh ekosistem [Methods of diagnosis of terrestrial ecosystems]. Rostov‐on‐Don, SFU Publ., 2016, 356 p. (In Russian)

30. Stepniewska Z., Wolinska A., Ziomek J. Response of soil catalase activity to chromium con‐ tamination. J. Environ. Sci. (China), 2009, vol. 21, iss. 8, pp. 1142‐1147. Doi: 10.1109/ISWREP.2011.5893614

31. Achuba F.I., Peretiemo‐Clarke B.O. Effect of spent engine oil on soil catalase and dehydrogenase activities. International Agrophysics, 2008, no. 22, pp. 1‐4.

32. Bouchez M., Blanchet D., Vandecasteele J‐P. Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism. Applied Microbiology and Biotechnology, 1995, vol. 43, iss. 1, pp. 156‐164. Doi: 10.1007/BF00170638