ANALYSIS OF THE INTERRELATION OF CYTOGENETIC HOMEOSTASIS AND OXIDATIVE STRESS IN THE ORGANISM OF GOBY FISH (GOBIIDAE) OF THE NORTHERN CASPIAN
https://doi.org/10.18470/1992-1098-2018-2-64-72
Abstract
Aim. The aim is to investigate the relationship between the content of micronuclei, destructive disorders in the nuclei of erythrocytes of peripheral blood of the goby fish and intensity of lipid peroxidation in fish tissues. Methods. Was used the method of quantitative accounting of morphologically altered red blood cells of peripheral blood of goby fish. Evaluation of cytogenetic disorders in fish blood cells was carried out based on a micronucleus test. Samples of muscle tissues and liver were sampled for biochemical analyzes. The rates of lipid peroxidation (LPO level) were determined by a method based on the reaction of malondialdehyde and thiobarbituric acid, the end product of lipid peroxidation. Results. A correlation was found between the rates of spontaneous LPO, ascorbate-dependant LPO and the accumulation of malondialdehyde in the liver of fish and changes in the nucleus of erythrocytes (R2 =0,8; R2 =0,6; R2 =0,7, respectively). Conclusions. We established the functional dependence which justifies the need to use cytogenetic markers to assess the impact of adverse environmental factors on the body of hydrobionts.
About the Authors
Tatiana V. Kuzina
Shirshov Institute of Oceanology, Russian Academy of Sciences,
Moscow
Russian Federation
Candidate of Biological Sciences, research associate
Russian Federation
Candidate of Biological Sciences, research associate
Maya L. Galaktionova
Shirshov Institute of Oceanology, Russian Academy of Sciences,
Moscow
Russian Federation
research associate
Russian Federation
research associate
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Review
For citations:
Kuzina T.V., Galaktionova M.L. ANALYSIS OF THE INTERRELATION OF CYTOGENETIC HOMEOSTASIS AND OXIDATIVE STRESS IN THE ORGANISM OF GOBY FISH (GOBIIDAE) OF THE NORTHERN CASPIAN. South of Russia: ecology, development. 2018;13(2):64-72. (In Russ.) https://doi.org/10.18470/1992-1098-2018-2-64-72
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