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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ecodag</journal-id><journal-title-group><journal-title xml:lang="ru">Юг России: экология, развитие</journal-title><trans-title-group xml:lang="en"><trans-title>South of Russia: ecology, development</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1992-1098</issn><issn pub-type="epub">2413-0958</issn><publisher><publisher-name>State Institute of Applied Ecology of the Republic of Dagestan</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18470/1992-1098-2016-2-50-58</article-id><article-id custom-type="elpub" pub-id-type="custom">ecodag-828</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЭКОЛОГИЯ ЖИВОТНЫХ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ECOLOGY OF ANIMALS</subject></subj-group></article-categories><title-group><article-title>O РОЛИ ДИКИХ ПТИЦ В СОХРАНЕНИИ И РАСПРОСТРАНЕНИИ ПТИЧЬЕГО ПАРАМИКСОВИРУСА СЕРОТИПА 1 (ВИРУС БОЛЕЗНИ НЬЮКАСЛА) НА ТЕРРИТОРИИ СИБИРИ И ДАЛЬНЕГО ВОСТОКА, РОССИЯ</article-title><trans-title-group xml:lang="en"><trans-title>THE ROLE OF WILD BIRDS IN PRESERVATION AND PREVALENCE OF AVIAN PARAMYXOVIRUS SEROTYPE 1 (NEWCASTLE DISEASE VIRUSES) IN SIBERIA AND THE FAR EAST, RUSSIA</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Глущенко</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Glushchenko</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник лаборатории экспериментального моделирования и патогенеза инфекционных заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, ул. Тимакова, 2, Новосибирск, 630060 Россия</p></bio><bio xml:lang="en"><p>Junior researcher of the laboratory of experimental modeling and pathogenesis of infectious diseases, Research Institute of Experimental and Clinical Medicine, 2 Timakova St. Novosibirsk, 630060 Russia</p></bio><email xlink:type="simple">rimmaaltai@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юрченко</surname><given-names>К. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Yurchenko</surname><given-names>K. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>аспирант, младший научный сотрудник лаборатории структурных основ патогенеза социально значимых заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, Новосибирск, Россия</p></bio><bio xml:lang="en"><p>PhD student, Junior researcher of laboratory of structural bases of thepathogenesis of socially significant diseases, Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юрлов</surname><given-names>А. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Yurlov</surname><given-names>A. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.б.н., ведущий научный сотрудник, руководитель группы экологии птиц, Институт систематики и экологии животных СО РАН, Новосибирск, Россия.</p></bio><bio xml:lang="en"><p>PhD in Biological sciences, Leading researcher, Head of the research group of bird ecology, Institute of systematic and ecology of animals SB RAS, Novosibirsk, Russia</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юшков</surname><given-names>Ю. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Yushkov</surname><given-names>Yu. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д.с.-х.н., старший научный сотрудник, заведующий лабораторией болезней птиц, Институт экспериментальной ветеринарии Сибири и Дальнего Востока, Новосибирск, Россия</p></bio><bio xml:lang="en"><p>Doctor of agricultural sciences, Senior researcher, Head of laboratory of avian disease, Institute of experimental veterinary of Siberia and the Far East, Novosibirsk, Russia</p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Щелканов</surname><given-names>М. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Shchelkanov</surname><given-names>M. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>профессор, доктор биологических наук, профессор кафедры Дальневосточного федерального университета, Владивосток, Россия</p></bio><bio xml:lang="en"><p>professor, Doctor of biological sciences, professor of the department of Far Eastern Federal University, Vladivostok, Russia</p></bio><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шестопалов</surname><given-names>А. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Shestopalov</surname><given-names>A. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>профессор, доктор биологических наук, заведующий лабораторией экспериментального моделирования и патогенеза инфекционных заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, Новосибирский национальный исследовательский государственный Университет, Новосибирск, Россия.</p></bio><bio xml:lang="en"><p>professor, Doctor of biological sciences, Head of the laboratory of experimental modeling and pathogenesis of infectious diseases, Research Institute of Experimental and Clinical Medicine, Novosibirsk state University, Novosibirsk, Russia</p></bio><xref ref-type="aff" rid="aff-6"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">лаборатория экспериментального моделирования и патогенеза инфекционных заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Laboratory of experimental modeling and pathogenesis of infectious diseases, Research Institute of Experimental and Clinical Medicine,  Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">лаборатория структурных основ патогенеза социально значимых заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Laboratory of structural basesof the pathogenesis ofsocially significant diseases, Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">группа экологии птиц, Институт Систематики и экологии животных Сибирское отделение РАН, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Research group of bird ecology Institute of systematic and ecology of animals SB RAS, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru">лаборатория болезней птиц, Институт экспериментальной ветеринарии Сибири и Дальнего Востока, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Laboratory of avian disease, Institute of experimental veterinary of Siberia and the Far East, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru">кафедра современных методов диагностики и медицинских технологий, Дальневосточный федеральный университет, Владивосток, Россия&#13;
&#13;
Биолого-почвенный институт ДВО РАН, Владивосток, Россия<country>Россия</country></aff><aff xml:lang="en">Department of modern methods of diagnostics and medical technology, Far Eastern Federal University, Vladivostok, Russia&#13;
&#13;
Institute of Biology and Soil Science FEB RAS, Vladivostok, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-6"><aff xml:lang="ru">лаборатория экспериментального моделирования и патогенеза инфекционных заболеваний, Научно-исследовательский институт экспериментальной и клинической медицины, Новосибирск, Россия&#13;
&#13;
Новосибирский национальный исследовательский государственный Университет, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Laboratory of experimental modeling and pathogenesis of infectious diseases, Research Institute of Experimental and Clinical Medicine,  Novosibirsk, Russia&#13;
&#13;
Novosibirsk state University, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>19</day><month>07</month><year>2016</year></pub-date><volume>11</volume><issue>2</issue><fpage>50</fpage><lpage>58</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Глущенко А.В., Юрченко К.С., Юрлов А.К., Юшков Ю.Г., Щелканов М.Ю., Шестопалов А.М., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Глущенко А.В., Юрченко К.С., Юрлов А.К., Юшков Ю.Г., Щелканов М.Ю., Шестопалов А.М.</copyright-holder><copyright-holder xml:lang="en">Glushchenko A.V., Yurchenko K.S., Yurlov A.K., Yushkov Y.G., Shchelkanov M.Y., Shestopalov A.M.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://ecodag.elpub.ru/ugro/article/view/828">https://ecodag.elpub.ru/ugro/article/view/828</self-uri><abstract><sec><title>Цель</title><p>Цель. Изучить экологическое разнообразие диких птиц на территории Сибири и Дальнего Востока, являющихся переносчиками вируса болезни Ньюкасла, который относится к потенциально опасным возбудителям заболевания для птицеводства.</p></sec><sec><title>Методы</title><p>Методы. Биологический материал в виде клоакальных смывов и фрагментов кишечника от диких мигрирующих птиц был собран в период 2008-2014 гг. Вирус был наработан в аллантоисной полости развивающихся куриных эмбрионов. Наличие вируса определяли в реакции гемагглютинации, а первичную идентификацию вируса болезни Ньюкасла подтверждали методом ОТ-ПЦР. Патогенность полученных изолятов была определена в тестах ICPI и MDT.</p></sec><sec><title>Результаты</title><p>Результаты. Было собрано и исследовано 4443 проб, полученных от диких мигрирующих птиц 11 отрядов. Вирус болезни Ньюкасла выявлен у 40 птиц из четырех отрядов. Основную роль в циркуляции ВБН на территории Сибири и дальнего Востока играют представители семейства Утиных (Anatidae) отряда Гусеобразных (Anseriformes), а именно виды – чирок-свистунок (Anas crecca), чирок-трескунок (Anas querquedula), кряква (Anas platyrhynchos) и широконоска (Anas clypeata). Все изоляты ВБН апатогены за исключением двух задепонированных штаммов. Заключение. Дикие мигрирующие птицы отрядов Гусеобразные (Anseriformes) и Ржанкообразные (Charadriiformes) являются носителями вируса болезни Ньюкасла и могут занести патогенные варианты вируса на территорию России.</p></sec></abstract><trans-abstract xml:lang="en"><p>The aim is to evaluate ecological diversity of wild birds in Siberia and the Russian Far East, which are carriers of Newcastle disease virus that belongs to potentially dangerous pathogen for poultry.</p><sec><title>Methods</title><p>Methods. Biological materials (cloacal swabs and intestinal fragments) of wild migratory birds were collected in 2008-2014. The viral isolates were propagated in the allantoic cavity of developing chicken embryos. The presence of virus was determined in hemagglutination tests and primary identification of Newcastle disease virus was confirmed by RT-PCR. Pathogenicity of the obtained isolates was determined in tests ICPI and MDT.</p></sec><sec><title>Results</title><p>Results. 4443 samples were obtained from wild migratory birds of 11 avian orders and were investigated. Newcastle disease virus was detected in 40 birds from 4 orders. The Duck family (Anatidae) of the Waterfowl order (Anseriformes) plays the leading role in the circulation of Newcastle disease virus in Siberia and the Far East.The main species among them - a teal (Anas crecca), a garganey (Anas querquedula), a mallard (Anas platyrhynchos) and a shoveler (Anas clypeata). All studied isolatesof Newcastle disease virus are apatogenic except for two deponated strains.</p></sec><sec><title>Main conclusions</title><p>Main conclusions. Wild migratory birds from orders Anseriformes and Charadriiformes are capable of carrying Newcastle diseasevirus and could transfer pathogenic variants of this virus to the Russian territory.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>вирус болезни Ньюкасла</kwd><kwd>дикие птицы</kwd><kwd>МЭБ – международное эпизоотическое бюро</kwd><kwd>патогенность</kwd><kwd>распространение</kwd><kwd>наблюдение</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Newcastle disease virus</kwd><kwd>wild birds</kwd><kwd>OIE - World Organisation for Animal Health</kwd><kwd>pathogenicity</kwd><kwd>prevalence</kwd><kwd>surveillance</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Mayo M.A. A summary of taxonomic changes recently approved by ICTV. Arch. Virol. 2002. Vol.147. 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