ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2026-2-11ecodag-3717Research ArticleЭКОЛОГИЯECOLOGYПотенциал штаммов Pseudomonas chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 для биоконтроля гриба Fusarium graminearum и его микотоксиновPotential of Pseudomonas chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 strains for biocontrol of Fusarium graminearum and its mycotoxinshttps://orcid.org/0000-0003-4281-5278СидороваТ. М.SidorovaT. M.

Татьяна М. Сидорова, кандидат биологических наук, старший научный сотрудник

350039 г. Краснодар, ул. Калинина, 62

Тел.+79280387012

Tatyana M. Sidorova, PhD (Biology), Senior Researcher

62 Kalinina St, Krasnodar, 350039

Tel. +79280387012

0166505@mail.ruhttps://orcid.org/0000-0003-3712-1343АстаховМ. М.AstakhovM. M.

Михаил М. Астахов

Краснодар

Mikhail M. Astakhov

Krasnodar

https://orcid.org/0000-0003-1937-286XГогинаН. Н.GoginaN. N.

Надежда Н. Гогина

Сергиев‐Посад

Nadezhda N. Gogina

Sergiev Posad

https://orcid.org/0000-0002-0060-1995АсатуроваА. М.AsaturovaA. M.

Анжела М. Асатурова

Краснодар

Angela M. Asaturova

Krasnodar

Федеральный научный центр биологической защиты растенийРоссияFederal Scientific Centre for Biological Plant ProtectionRussian FederationВсероссийский научно‐исследовательский и технологический институт птицеводстваРоссияFederal Scientific Centre, All‐Russian Research and Technological Institute of Poultry FarmingRussian Federation202603082026212125‐133Copyright © Сидорова Т.М., Астахов М.М., Гогина Н.Н., Асатурова А.М., 20262026Сидорова Т.М., Астахов М.М., Гогина Н.Н., Асатурова А.М.Sidorova T.M., Astakhov M.M., Gogina N.N., Asaturova A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3717

Проведена in vitro оценка влияния жидкой культуры (ЖК) и супернатанта бактерий P. chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 на содержание ДНК гриба F. graminearum и микотоксинов ДОН, 3‐ацетил‐ДОН, 15‐ацетил‐ДОН, ДОН‐3‐гликозид, ниваленол, фузаренон Х, ЗЕН, α‐зеараленол, β‐зеараленол, а также на степень корреляции этих показателей.Исследования выполнены с использованием адаптированного СTАВ‐метода и ВЭЖХ с масс‐спектрометрическим детектированием. ЖК и супернатант бактерий P. chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 не снижают содержание ДНК гриба F. graminearum 60318, зачастую отмечается даже стимулирование синтеза ДНК (на 5–12 %). Супернатант Pseudomonas sp. BZR 523‐2 способствует значительному увеличению содержания ДНК гриба F. graminearum, почти в 2 раза (на 96 %) по сравнению с контролем. Оба штамма Pseudomonas существенно не изменяют содержание ДОН. Содержание 15‐ацетил‐ДОНа и 3‐ацетил‐ДОНа подавляется супернатантами обоих исследуемых штаммов (на 14–28 %), особенно Pseudomonas sp. BZR 523‐2 (на 32 %). ЖК и супернатанты бактерий в значительной степени (до 94–115 %) способствуют накоплению ЗЕН и его производных (α‐ и β‐зеараленола).Обнаруженная существенная (от умеренной до высокой) корреляционная связь между ДНК гриба и количеством 15‐ и 3‐ацетил‐ДОН, а также между ДНК гриба и количеством α‐ и β‐зеараленола свидетельствует о наличии у штаммов P. chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 потенциала для биоконтроля гриба F. graminearum и его микотоксинов.

In vitro evaluation was conducted assessment of the effect of liquid culture (LC) and supernatant of P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 on the DNA content of F. graminearum and the mycotoxins DON, 3‐acetyl‐DON, 15‐acetyl‐DON, DON‐3‐glycoside, nivalenol, fusarenon X, ZEN, α‐zearalenol, β‐zearalenol and the degree of correlation between these parameters.The studies were performed using an adapted CTAB method and HPLC with mass spectrometric detection.LC and supernatant of P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 do not reduce the DNA content of F. graminearum 60318; DNA synthesis is often stimulated (by 5–12 %). Supernatant of Pseudomonas sp. BZR 523‐2 significantly increased the DNA content of the fungus F. graminearum, almost twofold (by 96 %) compared to the control. Both Pseudomonas strains did not significantly alter DON content. The content of 15‐acetyl‐DON and 3‐acetyl‐DON was suppressed by the supernatants of both strains (by 14–28 %), especially by Pseudomonas sp. BZR 523‐2 (by 32 %). FAs and bacterial supernatants significantly (up to 94–115 %) promoted the accumulation of ZEN and its derivatives (α‐ and β‐zearalenol).The observed significant (moderate to high) correlation between fungal DNA and the amount of 15‐ and 3‐acetyl‐DON, as well as between fungal DNA and the amount of α‐ and β‐zearalenol, indicates that P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 strains have potential for biocontrol of the fungus F. graminearum and its mycotoxins.

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The authors declare that there are no conflicts of interest present.