ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2025-3-18ecodag-3531Research ArticleБИОЛОГИЧЕСКИЕ РЕСУРСЫBIOLOGICAL RESOURCESГенетическое разнообразие кандидатных маркеров у овец локальной породы Дагестана в контексте адаптации к горным пастбищным экосистемамGenetic diversity of candidate markers in a local sheep breed from Dagestan in the context of adaptation to mountain pastoral ecosystemshttps://orcid.org/0009-0003-8713-2005КараеваИ. С.KaraevaI. S.

Индира С. Караева

 Махачкала 

Indira S. Karaeva 

Makhachkala 

https://orcid.org/0000-0003-2150-2192ОздемировА. А.OzdemirovA. A.

Алимсолтан А. Оздемиров, заведующий лабораторией геномных исследований, селекции и племенного дела

367014, г. Махачкала, ул. А. Шахбанова, 30. Тел. +79094806199

Alimsoltan A. Ozdemirov, Head, Laboratory of Genomic Research, Selection and Breeding

30 A. Shakhbanova St., Makhachkala, 367014. Tel. +79094806199

alim72@mail.ruhttps://orcid.org/0000-0002-7303-0222ХожоковА. А.KhozhokovA. A.

Абдусалам А. Хожоков

Махачкала

Abdusalam A. Khozhokov 

Makhachkala 

Федеральный аграрный научный центр Республики ДагестанFederal Agrarian Scientific Centre, Republic of Dagestan202520112025203194200Copyright © Караева И.С., Оздемиров А.А., Хожоков А.А., 20252025Караева И.С., Оздемиров А.А., Хожоков А.А.Karaeva I.S., Ozdemirov A.A., Khozhokov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3531

Целью исследования было изучение полиморфизма генов гормона роста (GH), калпастатина (CAST), фактора роста и дифференциации 9 (GDF9) и кератиноассоциированного белка (KAP1.3) у овец локальной аборигенной породы Дагестана методом полимеразной цепной реакции с рестрикционным анализом фрагментов (PCR‐RFLP). Материалом исследования послужили образцы крови 59 животных, отобранные с соблюдением этических норм. Лабораторные исследования проводили в аккредитованной лаборатории, где ДНК выделяли методом фенол‐хлороформной экстракции с последующей очисткой и спектрофотометрической оценкой качества. Генотипи‐ рование выполняли с использованием специфических праймеров и эндонуклеаз рестрикции с последующим электрофоретическим разделением продуктов реакции. Установлено преобладание гомозиготных генотипов по генам GH, CAST и GDF9, сопровождающееся высокой гомозиготностью и низкой полиморфной информативностью (PIC) по этим локусам. Напротив, локус KAP1.3 характеризовался повышенным уровнем генетического разнообразия и умеренной полиморфностью (PIC=0,375). Полученные данные позволяют сфокусировать подходы к использованию изученных генетических маркеров в селекционной работе без снижения генетического разнообразия поголовья.

The aim of this study was to investigate the polymorphism of the growth hormone GH, calpastatin CAST, growth and differentiation factor 9 GDF9, and keratin associated protein 1.3 KAP1.3 genes in a local indigenous sheep breed from Dagestan using polymerase chain reaction with restriction fragment length polymorphism analysis PCR RFLP. The material comprised blood samples from 59 animals collected in compliance with ethical standards. Laboratory analyses were carried out in an accredited laboratory, where genomic DNA was extracted by phenol chloroform extraction followed by purification and spectrophotometric quality assessment. Genotyping was performed using gene specific primers and restriction endonucleases followed by electrophoretic separation of the reaction products. A predominance of homozygous genotypes at the GH, CAST and GDF9 loci was observed, which was associated with high homozygosity and low polymorphic information content PIC for these loci. In contrast, the KAP1.3 locus showed an increased level of genetic diversity and moderate polymorphism with PIC equal to 0.375. These findings provide a basis for refining approaches to the use of these genetic markers in breeding programmes without reducing the genetic diversity of the population.

Локальная аборигенная порода Дагестанагенетическое разнообразиекандидатные маркерыадаптациягорно‐отгонное животноводствопастбищные экосистемыполиморфическая информативность (PIC)Local indigenous Dagestan sheep breedgenetic diversitycandidate markersadaptationmountain transhumant sheep farmingpasture ecosystemspolymorphic information content (PIC)ReferencesHassanane M.S., Salem M.H., Abdel-Rahman S.Z. et al. Genetic diversity and structure of Egyptian sheep populations using microsatellite markers // Small Ruminant Research. 2015. V. 123. N 1. P. 102–109. https://doi.org/10.1016/j.smallrumres.2014.11.009Hassanane M.S., Salem M.H., Abdel-Rahman S.Z. et al. Genetic diversity and structure of Egyptian sheep populations using microsatellite markers. Small Ruminant Research, 2015, vol. 123, no. 1, pp. 102–109. https://doi.org/10.1016/j.smallrumres.2014.11.009Kijas J.W., Lenstra J.A., Hayes B. et al. Genome-wide analysis of the world's sheep breeds reveals high levels of historic mixture and strong recent selection // PLoS Biology. 2012. V. 10. N 2. Article ID e1001258. https://doi.org/10.1371/journal.pbio.1001258Kijas J.W., Lenstra J.A., Hayes B. et al. Genome-wide analysis of the world's sheep breeds reveals high levels of historic mixture and strong recent selection. PLoS Biology, 2012, vol. 10, no. 2, article ID e1001258. https://doi.org/10.1371/journal.pbio.1001258Celik S., Avanus K., Yilmaz O. Association of polymorphisms in the GH gene with growth traits in sheep // Indian Journal of Animal Research. 2017. V. 51. N 2. P. 337–341.Celik S., Avanus K., Yilmaz O. Association of polymorphisms in the GH gene with growth traits in sheep. Indian Journal of Animal Research, 2017, vol. 51, no. 2, pp. 337–341.Скорых Л.Н., Фомина И.О., Суржикова Е.С. и др. Полиморфизм генов гормона роста (GH) и кальпастатина (CAST) у мясошерстных овец // Главный зоотехник. 2020. N 7. С. 6–11. https://doi.org/10.33920/sel-03-2007-01Skorih L.N., Fomina I.O., Surjikova E.S. et al. Polymorphism of genes of Growth Hormone (GH) and Calpastatin (CAST) in wool-and-meat producing sheep. Head of Animal Breeding, 2020, no. 7, pp. 6–11. (In Russian) https://doi.org/10.33920/sel-03-2007-01Синица В.А., Гришина А.В., Курбатова А.С. Генетическая характеристика тонкорунных пород овец по гену CAST // Вестник Алтайского государственного аграрного университета. 2018. N 11. С. 34–38.Sinitsa V.A., Grishina A.V., Kurbatova A.S. Genetic characteristics of fine-wool sheep breeds by the CAST gene. Vestnik Altaiskogo gosudarstvennogo agrarnogo universiteta [Bulletin of the Altai State Agrarian University]. 2018, no. 11, pp. 34–38. (In Russian)Moghadaszadeh A., Mirhoseini S.Z., Mohammadabadi M. et al. Polymorphism of GDF9 and BMP15 genes and their association with litter size in sheep // Iranian Journal of Biotechnology. 2021. V. 19. N 4. Article ID: e2800. https://doi.org/10.30498/IJB.2021.2800Moghadaszadeh A., Mirhoseini S.Z., Mohammadabadi M. et al. Polymorphism of GDF9 and BMP15 genes and their association with litter size in sheep. Iranian Journal of Biotechnology, 2021, vol. 19, no. 4, article ID: e2800. https://doi.org/10.30498/IJB.2021.2800Gootwine E. Breed and strain differences in the efficiency of growth in sheep // Small Ruminant Research. 2020. V. 185. Article ID: 106088. https://doi.org/10.1016/j.smallrumres.2020.106088Gootwine E. Breed and strain differences in the efficiency of growth in sheep. Small Ruminant Research, 2020, vol. 185, article ID: 106088. https://doi.org/10.1016/j.smallrumres.2020.106088Vega-Pla J.L., Sanz A., Jugo B.M. et al. Genetic relationships and admixture between sheep breeds from southern Spain and northern Morocco revealed by microsatellite markers // Small Ruminant Research. 2022. V. 215. Article ID: 106736. https://doi.org/10.1016/j.smallrumres.2022.106736Vega-Pla J.L., Sanz A., Jugo B.M. et al. Genetic relationships and admixture between sheep breeds from southern Spain and northern Morocco revealed by microsatellite markers.Small Ruminant Research, 2022, vol. 215, article ID: 106736. https://doi.org/10.1016/j.smallrumres.2022.106736Sutarno S., Setiadi M.A., Sumantri C. et al. Polymorphism of calpastatin gene and its association with growth traits in local Indonesian sheep // Asian-Australasian Journal of Animal Sciences. 2016. V. 29. N 9. P. 1290–1296. https://doi.org/10.5713/ajas.15.0965Sutarno S., Setiadi M.A., Sumantri C. et al. Polymorphism of calpastatin gene and its association with growth traits in local Indonesian sheep. Asian-Australasian Journal of Animal Sciences, 2016, vol. 29, no. 9, pp. 1290–1296. https://doi.org/10.5713/ajas.15.0965Оздемиров А.А., Чижова Л.Н., Хожоков А.А., Суржикова Е.С., Догеев Г.Д., Абдулмагомедов С.Ш. Полиморфизм генов CAST, GH, GDF9 овец дагестанской горной породы // Юг России: экология, развитие. 2021. Т. 16. N 2. C. 39–44. https://doi.org/10.18470/1992-1098-2021-2-39-44Ozdemirov A.A., Chizhova L.N., Khozhokov A.A., Surzhikova E.S., Dogeev G.D., Abdulmagomedov S.Sh. Polymorphism of genes CAST, GH, GDF9 of sheep of the Dagestan mountain breed. South of Russia: ecology, development, 2021, vol. 16, no. 2, pp. 39–44. (In Russian) https://doi.org/10.18470/1992-1098-2021-2-39-44Notter D.R. Genetic aspects of reproduction in sheep // Reproduction in Domestic Animals. 2008. V. 43. N 2. P. 122–128. https://doi.org/10.1111/j.1439-0531.2008.01147.xNotter D.R. Genetic aspects of reproduction in sheep. Reproduction in Domestic Animals, 2008, vol. 43, no. 2, pp. 122–128. https://doi.org/10.1111/j.1439-0531.2008.01147.xRashidi A., Mokhtari M.S., Esmailizadeh A.K. Genetic parameter estimates for growth traits in Kermani sheep // Small Ruminant Research. 2008. V. 74. N 2–3. P. 165–171. https://doi.org/10.1016/j.smallrumres.2007.03.012Rashidi A., Mokhtari M.S., Esmailizadeh A.K. Genetic parameter estimates for growth traits in Kermani sheep. Small Ruminant Research, 2008, vol. 74, no. 2–3, pp. 165–171. https://doi.org/10.1016/j.smallrumres.2007.03.012Гаджиев З.К., Суржикова Е.С., Михайленко Т.Н., Евлагина Д.Д., Онищенко О.Н. Распределение частоты встречаемости аллелей гена кальпастатина у овец разных пород // Ветеринария и Зоотехния. 2023. N 5. С. 72–78. https://doi.org/10.28983/asj.y2023i5pp72-78Gadzhiev Z.K., Surzhikova E.S., Mikhailenko T.N., Evlagina D.D., Onishchenko O.N. Distribution of the frequency of occurrence of alleles by the calpastatin gene in sheep of different breeds (review). The Agrarian Scientific Journal, 2023, no. 5, pp. 72–78. https://doi.org/10.28983/asj.y2023i5pp72-78Miraei-Ashtiani S.R., Kohram H., Shahrbabak M.M. Polymorphism in exon 5 of the ovine GDF9 gene and its association with reproductive traits // South African Journal of Animal Science. 2019. V. 49. N 1. P. 1–6. https://doi.org/10.4314/sajas.v49i1.1Miraei-Ashtiani S.R., Kohram H., Shahrbabak M.M. Polymorphism in exon 5 of the ovine GDF9 gene and its association with reproductive traits. South African Journal of Animal Science, 2019, vol. 49, no. 1, pp. 1– 6. https://doi.org/10.4314/sajas.v49i1.1Kharzinova V.R., Dotsev A.V., Sermyagin A.A. et al. Genetic diversity and population structure of Russian sheep breeds based on microsatellite analysis // Russian Journal of Genetics. 2021. V. 57. N 2. P. 231–242. https://doi.org/10.1134/S1022795421020076Kharzinova V.R., Dotsev A.V., Sermyagin A.A. et al. Genetic diversity and population structure of Russian sheep breeds based on microsatellite analysis. Russian Journal of Genetics, 2021, vol. 57, no. 2, pp. 231–242. https://doi.org/10.1134/S1022795421020076

The authors declare that there are no conflicts of interest present.