ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2025-3-9ecodag-3522Research ArticleЭКОЛОГИЯECOLOGYВысокогидроксилированные фуллеренолы С60(OH) 36 как потенциальные загрязнители окружающей среды: оценка антиоксидантной активности в клетках печениHighly hydroxylated fullerenols С60(OH) 36 as potential environmental pollutants: Evaluation of antioxidant activity in liverhttps://orcid.org/0009-0001-6318-595XИсрапиловаА. И.IsrapilovaA. I.

Ашура И. Исрапилова, аспирант 

367000, г. Махачкала, ул. М. Гаджиева, д. 45. Тел. +79331111529

Ashura I. Israpilova, postgraduate student 

Makhachkala

ms.israpilova98@bk.ruhttps://orcid.org/0000-0001-8868-4782АдиеваА. А.AdievaA. A.

Айна А. Адиева

Махачкала

Aina A. Adieva

Makhachkala

https://orcid.org/0000-0001-7744-859XДжафароваА. М.DzhafarovaA. M.

Альбина М. Джафарова 

Махачкала 

Albina M. Dzhafarova

Makhachkala

https://orcid.org/0000-0003-3979-4293ГусейноваН. О.GuseynovaN. O.

Надира О. Гусейнова 

Махачкала 

Nadira O. Guseynova

Makhachkala

https://orcid.org/0000-0001-6925-2802ЛютоваЖ. Б.LyutovaZh. B.

Жанна Б. Лютова

Санкт‐Петербург; Гатчина

Zhanna B. Lyutova

St. Petersburg; Gatchina

https://orcid.org/0000-0002-7618-5617БорисенковаА. А.BorisenkovaA. A.

Алина А. Борисенкова 

Санкт‐Петербург; Гатчина

Alina A. Borisenkova 

St. Petersburg; Gatchina

https://orcid.org/0009-0006-5097-5908ЯхьяевР. Г.YahyaevR. H.

Рамазан Г. Яхьяев

Махачкала

Ramazan H. Yahyaev

Makhachkala

https://orcid.org/0000-0002-3551-1746АбдуллаевВ. Р.AbdullaevV. R.

Вагаб Р. Абдуллаев

Махачкала; Кизляр

Vagab R. Abdullaev

Makhachkala; Kizlyar

Прикаспийский институт биологических ресурсов ДФИЦ РАНCaspian Institute of Biological Resources, Dagestan Federal Research Centre, Russian Academy of SciencesДагестанский государственный университетDagestan State UniversityСанкт‐Петербургский государственный технологический институт (технический университет) ; Петербургский институт ядерной физики им. Б.П. Константинова НИЦ «Курчатовский институт»St. Petersburg State Institute of Technology (Technical University) ; B.P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre Kurchatov InstituteДагестанский государственный медицинский университетDagestan State Medical UniversityДагестанский государственный университет ; Филиал Дагестанского государственного университетаDagestan State University ; Branch of Dagestan State University202520112025203103114Copyright © Исрапилова А.И., Адиева А.А., Джафарова А.М., Гусейнова Н.О., Лютова Ж.Б., Борисенкова А.А., Яхьяев Р.Г., Абдуллаев В.Р., 20252025Исрапилова А.И., Адиева А.А., Джафарова А.М., Гусейнова Н.О., Лютова Ж.Б., Борисенкова А.А., Яхьяев Р.Г., Абдуллаев В.Р.Israpilova A.I., Adieva A.A., Dzhafarova A.M., Guseynova N.O., Lyutova Z.B., Borisenkova A.A., Yahyaev R.H., Abdullaev V.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3522

Целью данной работы было исследование антиоксидантных свойствфуллеренола С60(OH)36 в различных модельных системах in vitro, а также оценка протекторной эффективности фуллеренола в отношении белков и липидов гомогенатов печени.Эксперименты проводились на гомогенатах печени белых лабораторных крыс. На первоначальном этапе оценивалась антиоксидантная активность фуллеренола в различных концентрациях (от 1∙10‐5 до 0,2 мг/мл). Поскольку в системе аутоокисления адреналина достаточно высокую антиоксидантную активность показала концентрация фуллеренола 0,001 мг/мл, мы использовали ее для исследования антиоксидантной активности в других модельных системах, в которых проводилась искусственная индукция окислительного стресса.В дальнейшем в каждой модельной системе были получены контрольные значения, относительно которых судили об эффективности действия фуллеренола в качестве антиоксиданта. Оценивалась протекторная эффективность фуллеренола в отношении белков и липидов гомогенатов печени. Также проведено исследование исследование влияния фуллеренола на активность фермента супероксиддисмутазы. На модельной системе было показано, что фуллеренол предотвращает окислительные повреждения липидов.Исследование интенсивности окислительной модификации белков в модельной системе Fe+2/Н2О2 (среда Фентона) показало, что фуллеренол эффективно снижал прирост карбонильных групп. С60(ОН)36 в концентрации 0,001 мг/мл снижал уровень генерации АФК (активные формы кислорода) в митохондриях печени крыс, что выражалось в падении интенсивности флуоресценции АФК‐чувствительного зонда.

The aim of this work was to study the antioxidant properties of fullerenol C60(OH)36 in various in vitro model systems, as well as to evaluate the protective effectiveness of fullerenol against proteins and lipids of liver homogenates.The experiments were carried out on liver homogenates of whitelaboratory rats. At the initial stage, the antioxidant activity of fullerenol in various concentrations (from 1×10‐5 to 0.2 mg/ml) was evaluated. Since the fullerenol concentration of 0.001 mg/ml showed a sufficiently high antioxidant activity in the epinephrine autoxidation system, we used it to study the antioxidant activity in other model systems in which artificial induction of oxidative stress was performed.Subsequently, control values were obtained in each model system, relative to which the effectiveness of fullerenol as an antioxidant was judged. The protective efficacy of fullerenol against liver homogenate proteins and lipids was evaluated. The effect of fullerenol on the activity of the superoxide dismutase enzyme was also studied. Using the model system, it was shown that fullerenol prevents oxidative damage to lipids.A study of the intensity of oxidative modification of proteins in the Fe+2/H2O2 model system (Fenton's medium) showed that fullerenol effectively reduced the increase in carbonyl groups. C60(OH)36 at a concentration of 0.001 mg/ml reduced the rate of ROS generation in rat liver mitochondria, which resulted in a decrease in the fluorescence intensity of the ROS‐sensitive (reactive oxygen species) the probe.

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The authors declare that there are no conflicts of interest present.