ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2025-2-2ecodag-3436Research ArticleВИРУСОЛОГИЯVIROLOGYИнактивирующая и ингибирующая активность сухих этанольных экстрактов полыни (Artemisia spp.) на вирус простого герпеса второго типа in vitroInactivating and inhibitory activity of dry ethanol extracts of wormwood (Artemisia spp.) on herpes simplex virus of the 2 type in vitrohttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.КаzachinskaiaE. I.

Елена И. Казачинская, доктор биологических наук, ведущий научный сотрудник ФИЦ ФТМ СО РАН; ведущий научный сотрудник ФБУН ГНЦ ВБ «Вектор»

г. Новосибирск; 630559, Новосибирская область, р/п Кольцово 32-1. Тел. +79095307441

 

Еlena I. Каzachinskaia, Doctor of Biology,,Leading Researcher, Research Institute of Virology, FRCFTM, Siberian Branch, Russian Academy of Sciences;

32‐1 Koltsovo, Novosibirsk, Novosibirskiy region, Russia 630559. Tel. +79095307441

lena.kazachinsksia@mail.ruhttps://orcid.org/0000-0002-9224-9350ВеличкоВ. В.VelichkoV. V.

Виктория В. Величко

Новосибирск

Victoriya V. Velichko

Novosibirsk

https://orcid.org/0000-0002-0485-4818РомановаВ. Д.RomanovaV. D.

Валерия Д. Романова

Новосибирск

Valeriya D. Romanova

Novosibirsk

https://orcid.org/0000-0003-1904-7901КругловД. С.KruglovD. S.

Дмитрий С. Круглов

Новосибирск

Dmitriy S. Kruglov

Novosibirsk

https://orcid.org/0009-0009-0480-2311ПрокушеваД. Л.ProkushevaD. L.

Дарья Л. Прокушева

Новосибирск

Dar’ya L. Prokusheva

Novosibirsk

https://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovA. A.

Александр А. Чепурнов

Новосибирск

Аlexander А. Chepurnov

Novosibirsk

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.КоnonovaYu. V.

Юлия В. Кононова

Новосибирск

Yulia V. Коnonova

Novosibirsk

https://orcid.org/0000-0003-0431-5424ФуЛ.FuL.

Лифон Фу

Ведущая лаборатория микробиологии и иммунологии патогенов CAS

Пекин

Lifeng Fu

CAS Key Laboratory of Pathogen Microbiology and Immunology

Beijing

 

https://orcid.org/0000-0003-2460-9921ШаоШ.ShaoSh.

Шуай Шао

Пекин

 

Shuai Shao

Beijing

https://orcid.org/0000-0003-3945-5339ГуляеваМ. А.GulyaevaM. A.

Марина А. Гуляева

Новосибирск

Marina A. Gulyaeva

Novosibirsk

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovA. M.

Александр М. Шестопалов

Новосибирск

Аlexander М. Shestopalov

Novosibirsk

Научно-исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины; Государственный научный центр вирусологии и биотехнологии «Вектор»РоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Vector State Research Centre of Virology and Biotechnology, Federal Service for Surveillance in the Sphere, Consumers Rights Protection and Human WelfareRussian FederationНовосибирский государственный медицинский университетРоссияNovosibirsk State Medical UniversityRussian FederationНаучно-исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медициныРоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesRussian FederationResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesRussian FederationИнститут микробиологии Китайской академии наукКитайInstitute of Microbiology, Chinese Academy of SciencesChinaНациональный институт радиологической защиты, Центр по контролю за заболеваемостьюКитайNational Institute for Radiological ProtectionChina2025250720252021436Copyright © Казачинская Е.И., Величко В.В., Романова В.Д., Круглов Д.С., Прокушева Д.Л., Чепурнов А.А., Кононова Ю.В., Фу Л., Шао Ш., Гуляева М.А., Шестопалов А.М., 20252025Казачинская Е.И., Величко В.В., Романова В.Д., Круглов Д.С., Прокушева Д.Л., Чепурнов А.А., Кононова Ю.В., Фу Л., Шао Ш., Гуляева М.А., Шестопалов А.М.Каzachinskaia E.I., Velichko V.V., Romanova V.D., Kruglov D.S., Prokusheva D.L., Chepurnov A.A., Коnonova Y.V., Fu L., Shao S., Gulyaeva M.A., Shestopalov A.M.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3436

Цель – оценка in vitro инактивирующей и ингибирующей активности сухих этанольных экстрактов разных видов полыни (Artemisia spp.), произрастающих в природе Новосибирской области и Республики Алтай, на репликацию вируса простого герпеса 2 типа (Herpes simplex virus, HSV-2).

Собрано растительное сырье и приготовлены сухие этанольные экстракты разных видов полыни. Использован лабораторный штамм MS HSV-2. Анализ инактивирующей и ингибирующей активности экстрактов проводили на культуре клеток Vero Е6 по общепринятым методикам. Образец сравнения – лиофилизированный препарат ацикловир («ФАРМЛЕНД», Республика Беларусь). Содержание биологически активных веществ (БАВ) в экстрактах проводили методом прямой и дифференциальной спектрофотометрии.

Выявлена инактивирующая и ингибирующая активность сухих этанольных экстрактов Artemisia spp., растворенных в ДМСО, в диапазоне 50 %-ных эффективных концентраций (EC50) от          2,46±0,63 мкг/мл до 218,75±40,09 мкг/мл в реакциях прямая инактивация (нейтрализация) вируса и от 13,67±2,50 до     218,75±40,09 мкг/мл по схемам «профилактика» заражения и «лечение» инфицированных клеток, соответственно. Методом спектрофотометрии в ультрафиолетовой и видимой области света в экстрактах определено процентное содержание суммы флавоноидов в пересчете на рутин, полифенольных соединений в пересчете на катехин и суммы оксикоричных кислот в пересчете на хлорогеновую кислоту.

На основе полученных результатов можно сделать вывод о наличии в этанольных экстрактах Artemisia spp., растворенных в ДМСО, БАВ (флавоноидов, полифенольных соединений и оксикоричных кислот), способствующих инактивации свободных вирионов и эффективно действующих на репликацию HSV-2 внутри инфицированных клеток    in vitro.

Aim. In vitro assessment of the inactivating and inhibitory activity of dry ethanol extracts of various wormwood species (Artemisia spp.) native to the Novosibirsk region and Altai Republic on the replication of Herpes simplex virus type 2 (HSV-2).

Vegetable raw materials were collected and dry ethanol extracts of various types of wormwood were prepared. The laboratory strain MS HSV-2 was used. The inactivating and inhibitory activity of the extracts was analysed on Vero E6 cell culture according to generally accepted methods. The comparison sample is the lyophilized drug acyclovir (FARMLAND, Republic of Belarus). The content of biologically active substances (BAS) in the extracts was carried out by mass spectrometry.

The inactivating and inhibitory activity of dry ethanol extracts of     Artemisia spp. dissolved in DMSO was revealed in the range of 50 % effective concentrations (EC50) from 2.46±0.63 μg/ml to            218.75±40.09 μg/ml in the reactions of direct inactivation (neutralization) of the virus and from 13.67±2.50 to 218.75±40.09 μg/ml according to the schemes of "prevention" of infection and "treatment" of infected cells, respectively. The percentage of flavonoids in terms of rutin, polyphenolic compounds in terms of catechin and oxycoric acids in terms of chlorogenic acid was determined by spectrophotometry in extracts.

Based on the results obtained it can be concluded that ethanol extracts of Artemisia spp. dissolved in DMSO contain BAS (these are flavonoids, polyphenolic compounds and oxycoric acids) that promote the inactivation of free virions and effectively act on HSV-2 replication inside infected cells in vitro.

HSV-2виды полыниэтанольные экстрактыинактивирующая и ингибирующая активностьспектрофотометрияHSV-2wormwood species (spp.)ethanol extractsinactivating and inhibitory activitymass spectrometryРабота выполнена при финансовой поддержке проекта РНФ 23-64_00005 «Геномика и эволюция вирусных патогенов, вызывающих наиболее распространенные респираторные заболевания» и в рамках госзадания № 122032300158-5, выполняемого в НИИ вирусологии ФИЦ ФТМ.2. The work was supported by the Russian Science Foundation (project no. 23-64-00005) Genomics and Evolution of Viral Pathogens Causing the Most Common Respiratory Diseases, and within the framework of state task No. 122032300158-5 performed at the Research Institute of Virology of FRCFTM.ReferencesMcQuillan G., Kruszon-Moran D., Flagg E.W., Paulose-Ram R. Prevalence of Herpes Simplex Virus Type 1 and Type 2 in Persons Aged 14–49: United States, 2015–2016 // National Center for Health Statistics; Hyattsville, MD, USA: 2018. P. 1–8. CHS Data Brief. 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The authors declare that there are no conflicts of interest present.