ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2025-2-1ecodag-3434Research ArticleВИРУСОЛОГИЯVIROLOGYМолекулярно-биологические свойства штамма вируса болезни Ньюкасла вирулентного генотипа VII, впервые выделенного в 2008 году от дикой утки на территории Республики АдыгеяMolecular and biological properties of the Newcastle disease virus strain of virulent genotype VII, first isolated in 2008 from a wild duck in the Republic of Adygea, Russiahttps://orcid.org/0000-0002-5784-0073ГлущенкоА. В.GlushchenkoA. V.

Александра В. Глущенко, научный сотрудник, лаборатория экспериментальной онкологии и испытания фармацевтических средств, НИИ Вирусологии

630060 г. Новосибирск, ул. Тимакова, 2. Тел. +79134696677

 

Alexandra V. Glushchenko, Researcher, Laboratory of Experimental Oncology and Pharmaceutical Testing, Research Institute of Virology

2 Timakova St, Novosibirsk, Russia 630060. Tel. +79134696677

rimmaaltai2017@gmail.comhttps://orcid.org/0000-0002-0679-8493ЮрченкоК. С.YurchenkoK. S.

Ксения С. Юрченко

Новосибирск

Kseniya S. Yurchenko

Novosibirsk

https://orcid.org/0000-0001-6412-3622АдаменкоЛ. С.AdamenkoL. S.

Любовь С. Адаменко

Новосибирск

Lyubov S. Adamenko

Novosibirsk

https://orcid.org/0000-0001-8320-6298КаркавинЮ И.KarkavinYu. I.

Юрий И. Каркавин

Новосибирск

Yuriy I. Karkavin

Novosibirsk

Федеральный исследовательский центр фундаментальной и трансляционной медициныРоссияFederal Research Center of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesRussian Federation202525072025202613Copyright © Глущенко А.В., Юрченко К.С., Адаменко Л.С., Каркавин Ю.И., 20252025Глущенко А.В., Юрченко К.С., Адаменко Л.С., Каркавин Ю.И.Glushchenko A.V., Yurchenko K.S., Adamenko L.S., Karkavin Y.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3434

Цель: исследовать некоторые биологические свойства полученного штамма вируса болезни Ньюкасла NDV/Adygea/duck/12/2008, определить степень вирулентности, провести филогенетическое исследование.

Биологический материал от диких перелетных птиц был собран в     2008 г. в сезон охоты. Изоляция и культивирование выделенных штаммов были проведены в системе развивающихся курных эмбрионов (РКЭ). Первичную идентификацию, подтверждающую наличие геммагглютинирующего агента в аллантоисной жидкости, проводили в реакции гемагглютинации (РГА). Патогенность оценивали методами MDT и ICPI. Было проведено секвенирование, филогене-тический анализ, определен генотип исследуемого штамма.

Представлены результаты, изучения основных биологических свойств штамма вируса болезни Ньюкасла NDV/Adygea/duck/12/2008, выделенного от дикой перелетной птицы на территории Южного федерального округа. Согласно проведенному филогенетическому исследованию, штамм NDV/Adygea/duck/12/2008 принадлежит к генотипу VII генетическому классу 2. Тестами определения степени вирулентности MDT и ICPI, а также молекулярно-генетическим исследованием описанный штамм был отнесен к высокопатогенной группе.

Aim. To study some biological properties of the obtained strain of Newcastle disease virus NDV/Adygea/duck/12/2008, including the degree of virulence, and to conduct a phylogenetic study.

Biological material from wild migratory birds was collected in 2008 during the hunting season. Isolation and cultivation of the isolated strains were carried out in the system of developing chicken embryos (RCE). Primary identification confirming the presence of a hemagglutinating agent in the allantoic fluid was carried out in the hemagglutination reaction (HR). Pathogenicity was assessed by MDT and ICPI methods. Sequencing, phylogenetic analysis, the genotype of the studied strain was determined.

The results of studying the main biological properties of the Newcastle disease virus strain NDV/Adygea/duck/12/2008, isolated from wild migratory birds in the Southern Federal District, are presented. According to the phylogenetic study, the NDV/Adygea/duck/12/2008 strain belongs to genotype VII and genetic class 2. The MDT and ICPI virulence tests, as well as the molecular genetic study, classified the described strain as highly pathogenic.

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The authors declare that there are no conflicts of interest present.