ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-098-2024-3-4ecodag-3220Research ArticleВИРУСОЛОГИЯVIROLOGYПротивоопухолевый эффект штамма вируса болезни Ньюкасла NDV/Altai/pigeon/777/2010 на модели солидной карциномы ЛьюисаAntitumor effect of the Newcastle disease virus strain NDV/Altai/pigeon/777/2010 on a model of solid Lewis carcinomahttps://orcid.org/0000-0001-8320-6298КаркавинЮ. И.KarkavinYu. I.

Юрий И. Каркавин, младший научный сотрудник, лаборатория экспериментальной онкологии и испытания фармацевтических средств, НИИ Вирусологии

630060, г. Новосибирск, ул. Тимакова 2. Тел. +79538075349 

Yuriy I. Karkavin, junior researcher, Laboratory of Experimental Oncology and Pharmaceutical Testing, Research Institute of Virology

2 Timakova St, Novosibirsk, 630060. Tel. +79538075349

karkaviny@mail.ruhttps://orcid.org/0000-0001-6412-3622АдаменкоЛ. С.AdamenkoL. S.

Любовь С. Адаменко 

Новосибирск 

Lyubov S. Adamenko 

Novosibirsk 

https://orcid.org/0000-0002-0679-8493ЮрченкоК. С.YurchenkoK. S.

Ксения С. Юрченко 

Новосибирск 

Kseniya S. Yurchenko 

Novosibirsk 

https://orcid.org/0000-0002-5784-0073ГлущенкоА. В.GlushchenkoA. V.

Александра В. Глущенко 

Новосибирск 

Alexandra V. Glushchenko 

Novosibirsk 

ФГБНУ «Федеральный исследовательский центр фундаментальной и трансляционной медицины» (ФИЦ ФТМ)РоссияFederal Research Centre of Fundamental and Translational Medicine (FRC FTM)Russian FederationФГБНУ «Федеральный исследовательский центр фундаментальной и трансляционной медицины» (ФИЦ ФТМ) ; ФГБНУ «Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук» (ИХБФМ СО РАН)РоссияFederal Research Centre of Fundamental and Translational Medicine (FRC FTM) ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences (ICBFM SB RAS)Russian Federation2024211020241934454Copyright © Каркавин Ю.И., Адаменко Л.С., Юрченко К.С., Глущенко А.В., 20242024Каркавин Ю.И., Адаменко Л.С., Юрченко К.С., Глущенко А.В.Karkavin Y.I., Adamenko L.S., Yurchenko K.S., Glushchenko A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3220

Цель: оценить эффективность интратуморальных введений штамма вируса болезни Ньюкасла NDV/Altai/pigeon/777/2010, сравнить прогрессирование опухолевых узлов после виротерапии, проанализировать патоморфологические изменения в опухолевой ткани in vitro и in vivo.

Проводили однократные интратуморальные инъекции мезогенным штаммом вируса болезни Ньюкасла NDV/Altai/pigeon/777/2010, выделенным от сизого голубя на территории Сибири, аутбредным мышам линии C57Bl/6 в солидные узлы карциномы легких Льюиса, привитые подкожно. Затем оценивали динамику роста опухоли и проводили анализ патоморфологических изменений в опухолевой ткани.

Было показано, что однократные интратуморальные инъекции иммунокомпетентным мышам линии C57Bl/6 мезогенным штаммом вируса болезни Ньюкасла NDV/Altai/pigeon/777/2010 привели к усилению характерных патоморфологических изменений в опухолевой ткани привитой подкожно карциномы легких Льюиса и к снижению роста опухоли по сравнению с контрольной группой мышей in vivo. Было отмечено прямое цитотоксическое действие штамма ВБН на клеточную линию Vero E6 in vitro.

Результаты данного исследования свидетельствуют о том, что штамм ВБН NDV/Altai/pigeon/777/2010 обладает противоопухолевыми свойствами, что, возможно, позволит использовать его в клинических условиях в качестве эффективного противоопухолевого препарата.

Aim. To evaluate the efficacy of intratumoral administrations of the Newcastle disease virus strain NDV/Altai/pigeon/777/2010, to compare progression of the tumor nodes after virotherapy and to analyse pathomorphological changes in the tumor tissue in vitro and in vivo. Single intratumoral injections of the mesogenic strain of Newcastle disease virus NDV/Altai/pigeon/777/2010, isolated from a rock dove in Siberia, were done into outbred mice of the C57Bl/6 line into solid nodes of Lewis lung carcinoma that were grafted subcutaneously. Then the dynamics of tumor growth and pathomorphological changes in the tumor tissue were assessed and analyzed.

It was shown that single intratumoral injections into immunocompetent C57Bl/6 mice with the mesogenic strain of Newcastle disease virus NDV/Altai/pigeon/777/2010 led to an increase in peculiar pathomorphological changes in the tumor tissue of subcutaneously grafted Lewis lung carcinoma and to a decrease in tumor growth compared to the control group of mice in vivo. A direct cytotoxic effect of the NDV strain on the Vero E6 cell line in vitro was noted.

The results of this study indicate that the NDV strain NDV/Altai/pigeon/777/2010 has antitumor properties. This may enable clinical trials to prove its effectiveness as an antitumor drug.

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The authors declare that there are no conflicts of interest present.