ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2024-1-3ecodag-3062Research ArticleВИРУСОЛОГИЯVIROLOGYИсследование ингибирующей активности экстрактов, фракций и вторичных метаболитов Silene spp. (Caryophylaceae) и Serratula cupuliformis (Asteraceae) на «вход» простого герпеса 2 типа в чувствительные клетки линии VeroInvestigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophylaceae) and Serratula cupuliformis (Asteraceae) on the «entry» of herpes simplex type 2 into sensitive cells of the Vero linehttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.КаzachinskaiaE. I.

Елена И. Казачинская - доктор биологических наук, ведущий научный сотрудник ФИЦ ФТМ, ведущий научный сотрудник ФБУН ГНЦ ВБ «Вектор».

630559 Новосибирская область, Новосибирск, р/п Кольцово 32‐1. Тел. +79095307441

lena.kazachinskaia@mail.ruhttps://orcid.org/0000-0002-4417-8340ЗибареваЛ. Н.ZibarevaL. N.

Томск

Tomsk

https://orcid.org/0000-0002-4287-8327ФилоненкоЕ. С.FilonenkoE. S.

Томск

Tomsk

https://orcid.org/0000-0001-9102-6756ИвановаА. В.IvanovaA. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.КоnonovaYu. V.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovА. A.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovA. M.

Новосибирск

Novosibirsk

Научно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины (ФИЦ ФТМ) Министерства науки и высшего образования Российской Федерации; Государственный научный центр вирусологии и биотехнологии «Вектор» (ФБУН ГНЦ ВБ «Вектор»)РоссияResearch Institute of Virology, Federal Research Center of Fundamental and Translational Medicine; Vector State Research Centre of Virology and Biotechnology, Federal Service for the Oversight of Consumer Protection and Welfare (Rospotrebnadzor)Russian FederationНациональный исследовательский Томский государственный университет (ТГУ)РоссияNational Research Tomsk State UniversityRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» (ФБУН ГНЦ ВБ «Вектор»)РоссияVector State Research Centre of Virology and Biotechnology, Federal Service for the Oversight of Consumer Protection and Welfare (Rospotrebnadzor)Russian FederationНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины (ФИЦ ФТМ) Министерства науки и высшего образования Российской ФедерацииРоссияResearch Institute of Virology, Federal Research Center of Fundamental and Translational MedicineRussian Federation2024070420241913046Copyright © Казачинская Е.И., Зибарева Л.Н., Филоненко Е.С., Иванова А.В., Кононова Ю.В., Чепурнов А.А., Шестопалов А.М., 20242024Казачинская Е.И., Зибарева Л.Н., Филоненко Е.С., Иванова А.В., Кононова Ю.В., Чепурнов А.А., Шестопалов А.М.Каzachinskaia E.I., Zibareva L.N., Filonenko E.S., Ivanova A.V., Коnonova Y.V., Chepurnov А.A., Shestopalov A.M.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3062

Целью является анализ in vitro ингибирующей активности экстрактов, фракций и вторичных метаболитов растений рода Silene (Caryophylaceae) и Serratula cupuliformis (Asteraceae) на «вход» простого герпеса 2 типа (HSV‐2) в чувствительные клетки линии Vero.

Приготовлены этанольные экстракты и бутанольные фракции Silene spp. и Serratula cupuliformis. Выделены флавоноид шафтозид и экдистероид 20‐гидроксиэкдизон из Lychnis chalcedonica. Анализ БАВ выполнен методом ВЭЖХ. Образцы растворяли в дистиллированной воде или ДМСО. Лабораторный штамм MS HSV‐2 пассирован на культуре клеток Vero. Анализ ингибирующей активности растительных препаратов проводили на культуре клеток Vero методом прямой инактивации (нейтрализации) вирионов c инфекционным титром 103 БОЕ/мл.

Выявлена ингибирующая активность растительных препаратов в диапазоне 50 %‐ных эффективных концентраций (EC50) от 2,12±0,47 до 180,99±49,24 мкг/мл при растворении в воде и от 1,99±0,44 до 57,42±14,74 мкг/мл при растворении в ДМСО, что для некоторых образцов сопоставимо с EC50 препаратов сравнения (сухими этанольными экстрактами специи гвоздики, чаги и корня солодки). Полученные результаты предполагают наличие в исследуемых растительных препаратах БАВ, действующих деструктивно на вирионы HSV‐2 и влияющих на один из основных этапов его «жизненного» цикла – на «вход» вируса в чувствительные клетки.

In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene (Caryophylaceae) and Serratula cupuliformis (Asteraceae) to the «entry» of herpes simplex type 2 (HSV‐2) into sensitive cells of the Vero line.

Ethanol extracts and butanol fractions of Silene spp. and Serratula cupuliformis were prepared. The flavonoid shaftoside and the ecdysteroid 20‐hydroxyecdysone from Lychnis chalcedonica were isolated. For analysis of biologically active substances (BAS) the HPLC method was used. The samples were dissolved in distilled water or DMSO. The laboratory strain MS HSV‐2 was grown on Vero cell culture. In vitro analysis of the inhibitory activity of the herbal preparations on «entry» of HSV‐2 was performed in Vero cell culture by direct inactivation (neutralisation) of virions with 103 PFU /ml.

Effective concentrations (EC50) have been identified in the range of 50 % from 2.12±0.47 to 180.99±49.24 μg/ml when preparations were dissolved in water and from 1.99±0.44 to 57.42±14.74 μg/ml when they were dissolved in DMSO. Such results for some samples is comparable to the EC50 of comparison preparations (dry ethanol extracts of spice of cloves, chaga and licorice root). The results obtained suggest the presence of BAS in the herbal preparations studied that act destructively on HSV‐2 virions and affect one of the main stages of its «life» cycle – the «entry» of the virus into sensitive cells.

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The authors declare that there are no conflicts of interest present.