ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2023-4-173-181ecodag-3010Research ArticleМЕДИЦИНСКАЯ ЭКОЛОГИЯMEDICAL ECOLOGYВклад энтеровирусов в этиологию острых респираторных вирусных инфекций у детей г. НовосибирскаEnteroviruses in the etiology of acute respiratory viral infections in children, Novosibirsk, Russiahttps://orcid.org/0000-0002-1931-2026КурскаяО. Г.KurskayaO. G.

Ольга Г. Курская, кандидат медицинских наук, старший научный сотрудник, руководитель лаборатории

630117

ул. Тимакова, 2

Новосибирск

Тел. +73833359405

Olga G. Kurskaya, PhD, Senior Researcher, Head of the Laboratory

630117

2 Timakova Street

Novosibirsk

Tel. +73833359405

kurskaya_og@mail.ruhttps://orcid.org/0000-0001-8071-5425СароянТ. А.SaroyanT. A.

Тереза А. Сароян

Новосибирск

Tereza A. Saroyan

Novosibirsk

https://orcid.org/0000-0002-7891-5847НоховаА. Р.NokhovaA. R.

Алина Р. Нохова

Новосибирск

Alina R. Nokhova

Novosibirsk

https://orcid.org/0000-0002-7780-1485ДубовицкийН. А.DubovitskiyN. A.

Никита А. Дубовицкий

Новосибирск

Nikita A. Dubovitskiy

Novosibirsk

https://orcid.org/0000-0002-5984-0819ДеркоА. А.DerkoA. A.

Анастасия А. Дерко

Новосибирск

Anastasia A. Derko

Novosibirsk

https://orcid.org/0009-0002-5546-5043БасоваП. С.BasovaP. S.

Полина С. Басова

Новосибирск

Polina S. Basova

Novosibirsk

https://orcid.org/0009-0001-4943-2619ГутоваТ. А.GutovaT. A.

Татьяна А. Гутова

Новосибирск

Tatiana A. Gutova

Novosibirsk

https://orcid.org/0000-0003-0736-0271СоломатинаМ. В.SolomatinaM. V.

Мария В. Соломатина

Новосибирск

Maria V. Solomatina

Novosibirsk

https://orcid.org/0000-0003-3719-4796МурашкинаТ. А.MurashkinaT. A.

Татьяна А. Мурашкина

Новосибирск

Tatiana A. Murashkina

Novosibirsk

https://orcid.org/0000-0002-3946-9872ШаршовК. А.SharshovK. A.

Кирилл А. Шаршов

Новосибирск

Kirill A. Sharshov

Novosibirsk

Федеральный исследовательский центр фундаментальной и трансляционной медициныРоссияFederal Research Center for Fundamental and Translational MedicineRussian Federation202312012024184173181Copyright © Курская О.Г., Сароян Т.А., Нохова А.Р., Дубовицкий Н.А., Дерко А.А., Басова П.С., Гутова Т.А., Соломатина М.В., Мурашкина Т.А., Шаршов К.А., 20242024Курская О.Г., Сароян Т.А., Нохова А.Р., Дубовицкий Н.А., Дерко А.А., Басова П.С., Гутова Т.А., Соломатина М.В., Мурашкина Т.А., Шаршов К.А.Kurskaya O.G., Saroyan T.A., Nokhova A.R., Dubovitskiy N.A., Derko A.A., Basova P.S., Gutova T.A., Solomatina M.V., Murashkina T.A., Sharshov K.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/3010   Цель

   Цель. Оценить вклад энтеровирусов человека в общую структуру острых респираторных вирусных инфекций у детей г. Новосибирска в течение эпидемического сезона 2022–2023 гг.

   Материал и методы

   Материал и методы. Проанализировано 1132 образца, собранных от детей 0–15 лет с симптомами ОРВИ, методом ПЦР в режиме реального времени на наличие генетического материала респираторных вирусов, включая энтеровирусы.

   Результаты

   Результаты. Положительными хотя бы на один вирус, включая энтеровирусы, были 65,2 % обследованных детей. Чаще всего у детей в указанный период встречался респираторно‐синцитиальный вирус, который составил 17,2 % от общего количества исследованных образцов. У 14,2 % обследованных детей выявлен вирус гриппа. Энтеровирусы были обнаружены в 9,2 % случаев, риновирус был выявлен у 10,8 % обследованных детей.

   Заключение

   Заключение. Респираторные энтеровирусы, наряду с риновирусом, занимают значительное место в структуре ОРВИ у детей. Изучение эпидемиологии энтеровирусов необходимо для понимания клинических  проявлений и исходов энтеровирусной инфекции, оценки бремени заболеваний, вызванных энтеровирусами, что важно для оптимизации профилактики и стратегий терапии.

   Aim

   Aim. To assess the contribution of human enteroviruses to the overall structure of acute respiratory viral infections in children in Novosibirsk during the epidemic season 2022–2023.

   Material and Methods

   Material and Methods. 1132 samples collected from children aged 0–15 years with symptoms of ARVI were analyzed by real–time PCR for the presence of genetic material of respiratory viruses, including enteroviruses.

   Results

   Results. 65.2 % of the examined children were positive for at least one virus, including enteroviruses. Respiratory syncytial virus was most often found in children during this period, which accounted for 17.2 % of the total number of samples studied. Influenza virus was detected in 14.2 % of the examined children. Enteroviruses were detected in 9.2 % of cases and rhinovirus was detected in 10.8 % of the examined children.

   Conclusions

   Conclusions. Respiratory enteroviruses, along with rhinovirus, occupy a significant place in the etiology of acute respiratory viral infections in children. The study of the epidemiology of enteroviruses is necessary to understand the clinical manifestations and outcomes of enterovirus infection, and to assess the burden of diseases caused by enteroviruses, which is important for optimizing prevention and therapy strategies.

энтеровирусыриновирусвирус гриппареспираторно‐синцитиальный вирусреспираторные вирусыострые респираторные вирусные инфекцииэтиологияenterovirusesrhinovirusinfluenza virusrespiratory syncytial virusrespiratory virusesacute respiratory viral infectionsetiologyИсследование выполнено при поддержке гранта РНФ № 23‐24‐00492The study was supported by the Russian Science Foundation grant No. 23‐24‐00492ReferencesSimmonds P., Gorbalenya A.E., Harvala H., Hovi T., Knowles N.J., Lindberg A.M., Oberste M.S., Palmenberg A.C., Reuter G., Skern T., Tapparel C., Wolthers K.C., Woo P.C.Y., Zell R. Recommendations for the nomenclature of enteroviruses and rhinoviruses // Archives of Virology. 2020. N 165. C. 793–797. doi: 10.1007/s00705-019-04520-6Simmonds P., Gorbalenya A.E., Harvala H., Hovi T., Knowles N.J., Lindberg A.M., Oberste M.S., Palmenberg A.C., Reuter G., Skern T., Tapparel C., Wolthers K.C., Woo P.C.Y., Zell R. Recommendations for the nomenclature of enteroviruses and rhinoviruses // Archives of Virology, 2020, no. 165, pp. 793–797. doi: 10.1007/s00705-019-04520-6Royston L., Tapparel C. Rhinoviruses and respiratory enteroviruses: not as simple as ABC // Viruses. 2016. N 8. P. 151–156. doi: 10.3390/v8010016Royston L,, Tapparel C. Rhinoviruses and respiratory enteroviruses: not as simple as ABC. Viruses, 2016, no. 8, pp. 151–156. doi: 10.3390/v8010016Fine J., Bray‐Aschenbrenner A., Williams H, Buchanan P., Werner J. The Resource Burden of Infections with Rhinovirus/Enterovirus, Influenza, and Respiratory Syncytial Virus in Children // Clin. Pediatr. 2018. N 58. C. 177–184.Fine J., Bray‐Aschenbrenner A., Williams H., Buchanan P., Werner J. The Resource Burden of Infections with Rhinovirus/Enterovirus, Influenza, and Respiratory Syncytial Virus in Children. Clinical Pediatrics. 2018, no. 58, pp. 177–184.Marjomäki V., Kalander K., Hellman M., Permi P. Enteroviruses and coronaviruses: similarities and therapeutict argets // Expert Opinion on Therapeutic Targets. 2021. V. 11. N 1. P. 23–27. doi: 10.1080/14728222.2021.1952985Marjomäki V., Kalander K., Hellman M., Permi P. Enteroviruses and coronaviruses: similarities and therapeutic targets. Expert Opinion on Therapeutic Targets, 2021, vol. 11, no. 1, pp. 23–27. doi: 10.1080/14728222.2021.1952985Tapparel C., Siegrist F., Petty T.J., Kaiser L. Picornavirus and enterovirus diversity with associated human diseases // Infection, Genetics and Evolution. 2013. N 14. P. 282–293. doi: 10.1016/j.meegid.2012.10.016Tapparel C., Siegrist F., Petty T.J. Picornavirus and enterovirus diversity with associated human diseases. Infection, Genetics and Evolution, 2013, no. 14, pp. 282–293. doi: 10.1016/j.meegid.2012.10.016Hayes A., Nguyen D., Andersson M., et al. A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts. Journal of Medical Virology. 2020. V. 92. P. 1065–1074. doi: 10.1002/jmv.25659Hayes A., Nguyen D., Andersson M., et al. A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts. Journal of Medical Virology, 2020, vol. 92, pp. 1065–1074. doi: 10.1002/jmv.25659Harvala H., Jasir A., Penttinen P., et al. Surveillance and laboratory detection for non‐polio enteroviruses in the European Union/European Economic Area, 2016 // Euro Surveill. 2017. V. 22. P. 233–236. doi: 10.2807/1560-7917.ES.2017.22.45.16-00807Harvala H., Jasir A., Penttinen P., et al. Surveillance and laboratory detection for non‐polio enteroviruses in the European Union/European Economic Area, 2016. Euro Surveill, 2017, vol. 22, pp. 233–236. doi: 10.2807/1560-7917.ES.2017.22.45.16-00807Holm‐Hansen C.C., Midgley S.E., Fischer T.K. Global emergence of enterovirus D68 : a systematic review // The Lancet Infectious Diseases. 2016. V. 16. N 5. P. 64–75. doi: 10.1016/S1473-3099(15)00543-5Holm‐Hansen C.C., Midgley S.E., Fischer T.K. Global emergence of enterovirus D68: a systematic review. The Lancet Infectious Diseases, 2016, vol.16, no. 5, pp. 64–75. doi: 10.1016/S1473-3099(15)00543-5Poelman R., Schuffenecker I., Van Leer‐Buter C., Josset L., Niesters H.G., Lina B. European surveillance for enterovirus D68 during the emerging North‐American outbreak in 2014 // Journal of Clinical Virology. 2015. V. 71. P. 1–9. doi: 10.1016/j.jcv.2015.07.296Poelman R., Schuffenecker I., Van Leer‐Buter C., Josset L., Niesters H.G., Lina B. European surveillance for enterovirus D68 during the emerging North‐American outbreak in 2014. Journal of Clinical Virology, 2015, vol. 71, pp. 1–9. doi: 10.1016/j.jcv.2015.07.296Harvala H., Broberg E., Benschop K., Berginc N., Ladhani Sh., Susi P., Christiansen Claus., McKenna J., Allen D., Makiello Ph., McAllister G., Carmen M., Zakikhany K., Dyrdak R., Nielsen Xiaohui, Madsen T., Paul J., Moore C., Karin von Eije, Piralla A., Fischer Thea K. Recommendations for enterovirus diagnostics and characterisation within and beyond Europe // Journal of Clinical Virology. 2018. V. 101. P. 11–17. doi: 10.1016/j.jcv.2018.01.008Harvala H., Broberg E., Benschop K., Berginc N., Ladhani Sh., Susi P., Christiansen Claus., McKenna J., Allen D., Makiello Ph., McAllister G., Carmen M., Zakikhany K., Dyrdak R., Nielsen Xiaohui, Madsen T., Paul J., Moore C., Karin von Eije, Piralla A., Fischer Thea K. Recommendations for enterovirus diagnostics and characterisation within and beyond Europe. Journal of Clinical Virology, 2018, vol. 101, pp. 11–17. doi: 10.1016/j.jcv.2018.01.008Fillatre A., François C., Segard C., et al. Epidemiology and seasonality of acute respiratory infections in hospitalized children over four consecutive years (2012– 2016) // Journal of Clinical Virology. 2018. T. 102. C. 27–31. doi: 10.1016/j.jcv.2018.02.010Fillatre A., François C., Segard C., et al. Epidemiology and seasonality of acute respiratory infections in hospitalized children over four consecutive years (2012– 2016). Journal of Clinical Virology, 2018, vol. 102, pp. 27–31. doi: 10.1016/j.jcv.2018.02.010Kurskaya O., Ryabichenko T., Leonova N., Shi W., Bi H., Sharshov K., Kazachkova E., Sobolev I., Prokopyeva E., Kartseva T., et al. Viral etiology of Acute Respiratory Infections in Hospitalized Children in Novosibirsk City, Russia (2013–2017) // PLoS ONE. 2018. V. 13. N 9. doi: 10.1371/journal.pone.0200117Kurskaya O., Ryabichenko T., Leonova N., Shi W., Bi H., Sharshov K., Kazachkova E., Sobolev I., Prokopyeva E., Kartseva T., et al. Viral etiology of Acute Respiratory Infections in Hospitalized Children in Novosibirsk City, Russia (2013–2017). PLoS ONE, 2018, vol. 13, no. 9. doi: 10.1371/journal.pone.0200117Kurskaya O.G., Prokopyeva E.A., Sobolev I.A., Solomatina M.V., Saroyan T.A., Dubovitskiy N.A., Derko A.A., Nokhova A.R., Anoshina A.V., Leonova N.V., et al. Changes in the Etiology of Acute Respiratory Infections among Children in Novosibirsk, Russia, between 2019 and 2022: The Impact of the SARS‐CoV‐2 Virus // Viruses. 2023. V. 15. P. 4. doi: 10.3390/v15040934Kurskaya O.G., Prokopyeva E.A., Sobolev I.A., Solomatina M.V., Saroyan T.A., Dubovitskiy N.A., Derko A.A., Nokhova A.R., Anoshina A.V., Leonova N.V., et al. Changes in the Etiology of Acute Respiratory Infections among Children in Novosibirsk, Russia, between 2019 and 2022: The Impact of the SARS‐CoV‐2 Virus. Viruses, 2023, vol. 15, iss. 4. doi: 10.3390/v15040934Baertl S., Pietsch C., Maier M., Hönemann M., Bergs S., Liebert U.G. Enteroviruses in Respiratory Samples from Paediatric Patients of a Tertiary Care Hospital in Germany // Viruses. 2021. V. 13. P. 5. doi: 10.3390/v13050882Baertl S., Pietsch C., Maier M., Hönemann M., Bergs S., Liebert U.G. Enteroviruses in Respiratory Samples from Paediatric Patients of a Tertiary Care Hospital in Germany. Viruses, 2021, vol. 13, iss. 5. doi: 10.3390/v13050882Poelman R., Schölvinck E.H., Borger R., Niesters H.G., Van Leer‐Buter C. The emergence of enterovirus D68 in a Dutch University Medical Center and the necessity for routinely screening for respiratory viruses // Journal of Clinical Virology. 2015. V. 62. P. 1–5.Poelman R., Schölvinck E.H., Borger R., Niesters H.G., Van Leer‐Buter C. The emergence of enterovirus D68 in a Dutch University Medical Center and the necessity for routinely screening for respiratory viruses. Journal of Clinical Virology. 2015, vol. 62, pp. 1–5.Andrés C., Vila J., Gimferrer L., Piñana M., Esperalba J., Codina M.G., Barnés M., Martín M.C., Fuentes F., Rubio S., et al. Surveillance of enteroviruses from paediatric patients attended at a tertiary hospital in Catalonia from 2014 to 2017 // Journal of Clinical Virology. 2019. V. 110. P. 29–35.Andrés C., Vila J., Gimferrer L., Piñana M., Esperalba J., Codina M.G., Barnés M., Martín M.C., Fuentes F., Rubio S., et al. Surveillance of enteroviruses from paediatric patients attended at a tertiary hospital in Catalonia from 2014 to 2017. Journal of Clinical Virology. 2019, vol. 110, pp. 29–35.Kiseleva I., Grigorieva E., Larionova N., Al Farroukh M, Rudenko L. COVID‐19 in Light of Seasonal Respiratory Infections // Biology. 2020. N 9. 240. DOI: 10.3390/biology9090240Kiseleva I., Grigorieva E., Larionova N., Al Farroukh M, Rudenko L. COVID‐19 in Light of Seasonal Respiratory Infections. Biology, 2020, no. 9, 240. DOI: 10.3390/biology9090240Kiseleva I., Ksenafontov A. COVID‐19 Shuts Doors to Flu but Keeps Them Open to Rhinoviruses // Biology. 2021. V. 10. P. 8. doi: 10.3390/biology10080733Kiseleva I., Ksenafontov A. COVID‐19 Shuts Doors to Flu but Keeps Them Open to Rhinoviruses. Biology, 2021, vol. 10, iss. 8. doi: 10.3390/biology10080733Cai X., Wang Q., Lin G., et al. Respiratory virus infections among children in South China // Journal of Medical Virology. 2014. V. 86. P. 1249–1255.Cai X., Wang Q., Lin G., et al. Respiratory virus infections among children in South China. Journal of Medical Virology. 2014, vol. 86, pp. 1249–55.Tabatabai J., Ihling C.M., Manuel B., Rehbein R.M., Schnee S.V., Hoos J., Pfeil J., Grulich‐Henn J., Schnitzler P. Viral Etiology and Clinical Characteristics of Acute Respiratory Tract Infections in Hospitalized Children in Southern Germany (2014–2018) // Open Forum Infectious Diseases. 2023. V. 10. N 3. DOI: 10.1093/ofid/ofad110Tabatabai J., Ihling C.M., Manuel B., Rehbein R.M., Schnee S.V., Hoos J., Pfeil J., Grulich‐Henn J., Schnitzler P. Viral Etiology and Clinical Characteristics of Acute Respiratory Tract Infections in Hospitalized Children in Southern Germany (2014–2018). Open Forum Infectious Diseases, 2023, vol. 10, no. 3. DOI: 10.1093/ofid/ofad110Fine J., Bray‐Aschenbrenner A., Williams H., Buchanan P., Werner J. The Resource Burden of Infections With Rhinovirus/Enterovirus, Influenza, and Respiratory Syncytial Virus in Children // Clinical Pediatrics (Phila). 2019. V. 58. Ed. 2. P. 177–184. DOI:10.1177/0009922818809483.Fine J., Bray‐Aschenbrenner A., Williams H., Buchanan P., Werner J. The Resource Burden of Infections With Rhinovirus/Enterovirus, Influenza, and Respiratory Syncytial Virus in Children. Clinical Pediatrics (Phila), 2019, vol. 58, no. 2, pp. 177–184. DOI: 10.1177/0009922818809483.

The authors declare that there are no conflicts of interest present.