ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2023-4-104-113ecodag-2997Research ArticleЭКОЛОГИЯ МИКРООРГАНИЗМОВИзучение влияния бактерий рода Pseudomonas на рост и токсинопродуцирование гриба Fusarium graminearum in vitroStudy of the effect of bacteria of the genus Pseudomonas on the growth and toxin production of the fungus Fusarium graminearum in vitrohttps://orcid.org/0000-0002-8679-6139АллахвердянВ. В.TolkachV. F.

Валерия В. Аллахвердян, м. н. с.

лаборатория микробиологической защиты растений

350039

п/о‐39

Краснодар

Тел. +79648950107

Valeria V. Allahverdyan, Junior Researcher

Laboratory of Microbiological Plant Protection

350039

p/o‐39

Krasnodar

lera_arm@mail.ruhttps://orcid.org/0000-0003-4281-5278СидороваТ. М.VolkovYu. G.

Татьяна М. Сидорова

Краснодар

Tatiana M. Sidorova

Krasnodar

https://orcid.org/0000-0002-8048-4740ТемердашевА. З.KakarekaN. N.

Азамат З. Темердашев

Краснодар

Azamat Z. Temerdashev

Krasnodar

https://orcid.org/0000-0002-0060-1995АсатуроваА. М.AlievM. R.

Анжела М. Асатурова

Краснодар

Anzhela M. Asaturova

Krasnodar

https://orcid.org/0000-0002-7297-5929ТомашевичН. С.ShchelkanovM. Yu.

Наталья С. Томашевич

Краснодар

Natalia S. Tomashevich

Krasnodar

Федеральный научный центр биологической защиты растенийFederal Scientific Centre for Biological Plant ProtectionКубанский государственный университетKuban State University202309012024184104113Copyright © Аллахвердян В.В., Сидорова Т.М., Темердашев А.З., Асатурова А.М., Томашевич Н.С., 20242024Аллахвердян В.В., Сидорова Т.М., Темердашев А.З., Асатурова А.М., Томашевич Н.С.Tolkach V.F., Volkov Y.G., Kakareka N.N., Aliev M.R., Shchelkanov M.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2997   Цель

   Цель. Изучить потенциал бактерий штаммов P. chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 для ингибирования роста возбудителей фузариоза колоса на примере гриба F. graminearum и снижения накопления дезоксиниваленола (ДОН) и зеараленона (ЗЕН) in vitro.

   Материалы и методы

   Материалы и методы. Анализ антигрибных метаболитов бактерий рода Pseudomonas осуществляли методом тонкослойной хроматографии и биоавтографии. Опыт по влиянию жидкой культуры и супернатанта бактерий на рост и токсинопродуцирование гриба F. graminearum in vitro проводили на зерне пшеницы. Анализ зерна на микотоксины осуществлялся методом ВЭЖХ‐МСВР.

   Результаты

   Результаты. Обнаружена способность штаммов P. chlororaphis BZR 245‐F и Pseudomonas sp. BZR 523‐2 продуцировать антигрибные метаболиты, при этом происходит ингибирование роста гриба F. graminearum 60318 in vitro. Содержание ДОН снижается как под действием жидкой культуры и супернатанта бактерий P. chlororaphis BZR 245‐F на 60 % и 70 %, соответственно, так и жидкой культуры и супернатанта бактерий Pseudomonas sp.BZR 523‐2 на 75 % и 90 %, соответственно. Накопление ЗЕН также значительно подавляется под влиянием жидких культур и супернатантов бактериальных штаммов. Количество ЗЕН снижается под действием жидкой культуры и супернатанта бактерий P. chlororaphis BZR 245‐F на 80 % и 95 %, соответственно. Жидкая культура и супернатант бактерий Pseudomonas sp. BZR 523‐2 подавляют накопление ЗЕН на 60 % и 84 %, соответственно.

   Заключение

   Заключение. Полученные результаты по исследованию влияния жидких культур и супернатантов бактерий штаммов P. chlororaphis BZR 245‐F и Pseudomonas sp.BZR 523‐2 на рост и токсинопродуцирование гриба F. graminearum 60318 in vitro позволяют рассматривать данные штаммы в качестве потенциальных продуцентов эффективных биофунгицидов против токсинопродуцирующих грибов. Необходимо провести дальнейшее изучение данных штаммов бактерий in vitro и in vivo на растениях.

   Aim

   Aim. To study the potential of bacteria strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to inhibit the growth of Fusarium ear blight pathogens through the example of the fungus F. graminearum and to reduce the accumulation of deoxynivalenol (DON) and zearalenone (ZEN) in vitro.

   Materials and Methods

   Materials and Methods. Antifungal metabolites of Pseudomonas bacteria were analysed by thin layer chromatography and bioautography. An experiment on the effect of liquid culture and supernatant of bacteria on the growth and toxin production of the fungus F. graminearum in vitro was carried out on wheat grain. Analysis of grain for mycotoxins was carried out by HPLC‐HRMS.

   Results

   Results. The ability of strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to produce antifungal metabolites was found, while inhibiting the growth of the fungus F. graminearum 60318 in vitro. The content of DON decreases both under the action of the liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 60 % and 70 %, respectively, and the liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria by 75 % and 90 %, respectively. The accumulation of ZEN is also significantly suppressed under the influence of liquid cultures and supernatants of bacterial strains. The amount of ZEN decreases under the influence of liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 80 % and 95 %, respectively. Liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria inhibited the accumulation of ZEN by 60 % and 84 %, respectively.

   Conclusion

   Conclusion. The results obtained through the study of the effect of liquid cultures and supernatants of P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 strains on the growth and toxin production of the fungus F. graminearum 60318 in vitro allow us to consider these strains as potential producers of effective biofungicides against toxin‐producing fungi. Further studies of these bacterial strains in vitro and in vivo on plants are needed.

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The authors declare that there are no conflicts of interest present.