ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2023-3-103-117ecodag-2913Research ArticleЭКОЛОГИЯ МИКРООРГАНИЗМОВСпособ получения водной вытяжки и сухих этанольных экстрактов травы (смесь цветков с листьями) и стебля зверобоя продырявленного (Hypericum perforatum L.), обладающих ингибирующей активностью на репликацию коронавируса SARS-CoV-2 in vitroA method for obtaining aqueous and dry ethanol extracts of grass (a mixture of flowers with leaves) and a stalk of St. John's wort (Hypericum perforatum L.) with inhibitory activity on the replication of the SARS‐CoV‐2 coronavirus in vitrohttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.КаzachinskaiaЕ. I.

Елена И. Казачинская, доктор биологических наук, ведущий научный сотрудник

630559, Новосибирская область, Новосибирск, р/п Кольцово 32‐1

Тел. +79095307441

Еlena I. Каzachinskaia, Doctor of Biology, Leading Researcher, Research Institute of Virology

Prospekt Koltsovo 32‐1, Novosibirsk, Novosibirskiy region, 630559

Теl. +79095307441

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.КоnonovaYu. V.

Юлия В. Кононова

Новосибирск

Yulia V. Коnonova

Novosibirsk

https://orcid.org/0000-0001-9102-6756ИвановаА. В.IvanovaA. V.

Алла В. Иванова

Новосибирск

Alla V. Ivanоva

Novosibirsk

https://orcid.org/0000-0002-4417-8340ЗибареваЛ. Н.ZibarevaL. N.

Лариса Н. Зибарева

Томск

Larisa N. Zibareva

Novosibirsk

https://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovА. А.

Александр А. Чепурнов

Новосибирск

Аlexander А. Chepurnov

Novosibirsk

https://orcid.org/0000-0002-6860-9168РоманюкВ. В.RomanyukV. V.

Владимир В. Романюк

Новосибирск

Vladimir V. Romanyuk

Novosibirsk

https://orcid.org/0009-0008-3921-0407БийболатовА. А.BiibolatovA. A.

Апенди А. Бийболатов

Махачкала

Apendi A. Biibolatov

Makhachkala

https://orcid.org/0000-0003-3945-5339ГуляеваМ. А.GulyaevaМ. А.

Марина А. Гуляева

Новосибирск

Мarina А. Gulyaeva

Novosibirsk

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovА. М.

Александр М. Шестопалов

Новосибирск

Махачкала

Аlexander М. Shestopalov

Novosibirsk

Makhachkala

Научно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины Министерства науки и высшего образования Российской Федерации; Государственный научный центр вирусологии и биотехнологии «Вектор»РоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine (FRCFTM), Siberian Branch; State Research Centre of Virology and Biotechnology, VECTOR, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareRussian FederationНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины Министерства науки и высшего образования Российской ФедерацииРоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine (FRCFTM), Siberian Branch, Russian Academy of SciencesRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор»РоссияState Research Centre of Virology and Biotechnology, VECTOR, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareRussian FederationНациональный исследовательский Томский государственный университетРоссияNational Research Tomsk State UniversityRussian FederationНаучно‐производственная фирма (НПФ) «Золотая долина»РоссияZolotaya Dolina Research and Production CompanyRussian FederationДагестанский государственный медицинский университетРоссияDagestan State Medical UniversityRussian FederationФакультет естественных наук Новосибирского государственного университетаРоссияDepartment of Natural Science, Novosibirsk State UniversityRussian FederationНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины Министерства науки и высшего образования Российской Федерации; Дагестанский государственный университетРоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine (FRCFTM), Siberian Branch, Russian Academy of Sciences; Dagestan State UniversityRussian Federation202302112023183103117Copyright © Казачинская Е.И., Кононова Ю.В., Иванова А.В., Зибарева Л.Н., Чепурнов А.А., Романюк В.В., Бийболатов А.А., Гуляева М.А., Шестопалов А.М., 20232023Казачинская Е.И., Кононова Ю.В., Иванова А.В., Зибарева Л.Н., Чепурнов А.А., Романюк В.В., Бийболатов А.А., Гуляева М.А., Шестопалов А.М.Каzachinskaia Е.I., Коnonova Y.V., Ivanova A.V., Zibareva L.N., Chepurnov А.А., Romanyuk V.V., Biibolatov A.A., Gulyaeva М.А., Shestopalov А.М.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2913

Цель. Оценка in vitro ингибирующей активности водных вытяжек и сухих этанольных экстрактов зверобоя продырявленного на репликацию SARSCoV-2 по трем схемам эксперимента – прямая инактивация (нейтра- лизация) вируса, а также «профилактика» и «лечение» клеток.Материалы и методы. Лабораторный штамм SARS-CoV-2/human/RUS/Nsk-FRCFTM-1/2020 пассирован на культуре клеток Vero. Приготовлены водные вытяжки и сухие этанольные экстракты частей растения Hypericum perforatum L., собранного в период цветения в Новосибирской области. Сухие экстракты растворяли в ДМСО. Образцы сравнения – сухие этанольные экстракты чаги, специи гвоздики и черного чая.Результаты. Показано, что водная вытяжка травы (смесь цветков с листьями) H. perforatum L. при прямой инактивации вируса активна в разведении 1/4096. Для сухого этанольного экстракта травы H. perforatum L., выявлены 50%-ные эффективные концентрации (EC50), равные 2,44±0,87; 8,79±1,91 и 14,65±1,91 мкг/мл, соответственно, при прямой инактивации, а также по «профилактической» схеме и при «лечении» клеток. С учетом цитотоксичности и в сравнении с контрольными образцами значения селективных индексов (SI50) исследуемых растительных препаратов при прямой инактивации были более высокие, чем при других схемах экспериментов и распределились следующим образом (по убывающей): 204,92; 153,68; 115,27; 32,01 и 21,33 для сухих этанольных экстрактов черного чая из Индии, cпеции гвоздики, травы (смесь цветков с листьями) зверобоя продырявленного, чаги и стебля зверобоя продырявленного, соответственно. Методом ВЭЖХ показано, что в этанольном экстракте травы H. perforatum L. содержится большее количество флавоноидов, чем в экстракте стеблей. Тем не менее, антивирусная активность выявлена и для экстракта стеблей этого растения с EC50, равными 14,65±1,91; 78,13±20,05 и 117,19±15,31 мкг/мл (по трем схемам экспериментов), соответственно.Заключение. Для приготовления антивирусных препаратов можно использовать в качестве сырья растение H. perforatum L. целиком, включая стебли.

Aim. In vitro evaluation of the inhibitory activity of aqueous extracts and dry ethanol extracts of St John's wort (Hypericum perforatum L.) on the replication of SARS-CoV-2 according to three experimental schemes – direct inactivation (neutralisation) of the virus as well as "prevention" and "treatment" of cells.Materials and Methods. The laboratory strain SARS-CoV-2/human/RUS/Nsk-FRCFTM-1/2020 was passed on Vero cell culture. Water extracts and dry ethanol extracts of parts of H. perforatum L. collected during the flowering period in the Novosibirsk region were prepared. Dry extracts were dissolved in DMSO. Comparison samples are dry ethanol extracts of chaga, cloves and black tea.Results. It is shown that the aqueous extract of grass (a mixture of flowers with leaves) of H. perforatum L. with direct inactivation of the virus it is active in dilution of 1/4096. For the dry ethanol extract of the herb H. perforatum L., 50 % effective concentrations (EC50) were found equal to 2.44±0.87; 8.79±1.91 and 14.65±1.91 μg/ml respectively with direct inactivation as well as according to the "preventive" scheme and with the "treatment" of cells. Taking into account cytotoxicity, as well as in comparison with control samples, the values of selective indices (SI50) of the studied herbal preparations during direct inactivation were higher than with other experimental schemes and were distributed as follows (in descending order): 204.92; 153.68; 115.27; 32.01 and 21.33 for dry ethanol extracts of black tea from India, cloves, herbs, a mixture of flowers with leaves, of H. perforatum, chaga and the stems of H. perforatum respectively. The HPLC method has shown that the ethanol extract of the herb H. perforatum L. contains a greater amount of flavonoids than the extract of stems. Nevertheless, antiviral activity was also detected for the extract of stems of this plant with EC50 equal to 14.65±1.91; 78.13±20.05 and 117.19±15.31 μg/ml (according to three experimental schemes), respectively.Conclusion. For the preparation of antiviral drugs the whole plant of H. perforatum L., including stems, can be used as raw materials.

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The authors declare that there are no conflicts of interest present.