ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2023-2-44-52ecodag-2825Research ArticleЭКОЛОГИЯ ГРИБОВECOLOGY OF FUNGIПервые современные находки краснокнижного гриба Battarrea phalloides (Agaricales, Basidiomycota) в Республике Дагестан, РоссияFirst modern findings of red‐listed fungus Battarrea phalloides (Agaricales, Basidiomycota) in the Republic of Dagestan, Russiahttps://orcid.org/0000-0003-1217-5548ВолобуевС. В.VolobuevS. V.

Сергей В. Волобуев, кандидат биологических наук, старший научный сотрудник лаборатории систематики и географии грибов

197022 г. Санкт‐Петербург, ул. Профессора Попова, 2. 

Тел. +78123725469 

Sergey V. Volobuev, Candidate of Sciences (Biology), Senior Researcher, Laboratory of Systematics and Geography of Fungi

2 Professora Popova St, Saint Petersburg, 197022

Tel. +78123725469 

sergvolobuev@binran.ruhttps://orcid.org/0000-0002-8733-2168ШаховаН. В.ShakhovaN. V.

Санкт‐Петербург

Saint‐Petersburg

Ботанический институт им. В.Л. Комарова РАНРоссияKomarov Botanical Institute of the Russian Academy of SciencesRussian Federation20231007202318244‐52Copyright © Волобуев С.В., Шахова Н.В., 20232023Волобуев С.В., Шахова Н.В.Volobuev S.V., Shakhova N.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2825Цель

Цель. Мониторинг известных, а также поиск новых местонахождений редких и охраняемых видов является необходимым условием ведения Красных книг и природоохранной деятельности в целом. Цель данной работы – представить описание находки базидиомицета Battarrea phalloides, редкого и охраняемого вида в Республике Дагестан, а также обсудить особенности его филогенетического разнообразия, распространения и экологии.

Материал и методы

Материал и методы. Первичная идентификация материала выполнена методами световой микроскопии. Филогенетический анализ проведен на основе метода максимального правдоподобия.

Результаты

Результаты. Выявлено новое местонахождение B. phalloides на территории Гунибского района Республики Дагестан. Приведено подробное описание макро‐ и микроморфологии собранной базидиомы, а также особенностей занимаемого данным видом нового экотопа. Получена и депонирована в международную базу NCBI GenBank полная нуклеотидная ITS1–5.8S–ITS2 области ярДНК. По итогам филогенетического анализа изученный образец сформировал отдельную кладу среди других представителей вида B. phalloides, имеющих различное географическое происхождение. 

Заключение

Заключение. Новая находка B. phalloides, зарегистрированная в Дагестане, позволила расширить знания об экологии и распространении, а также о внутривидовой филогенетической структуре краснокнижного в регионе и малоизвестного на Кавказе вида.

Aim

Aim. The monitoring of known as well as the search for new localities of rare and protected species is a requirement for Red Data Books and conservation efforts as a whole. The aim of this paper is to describe the finding of basidiomycete fungus Battarrea phalloides, a rare and protected species in the Republic of Dagestan, and to discuss features of its phylogenetic diversity, distribution and ecology.

Material and Methods

Material and Methods. Primary identification of the material was carried out by light microscopy. Phylogenetic analysis was performed based on the Maximum Likelihood method.

Results

Results. New locality of B. phalloides has been revealed in Gunibsky district of the Republic of Dagestan. A detailed description of macro‐ and micromorphology of the collected basidioma, as well as peculiarities of new ecotope occupied by the species is presented. The complete ITS1–5.8S–ITS2 nrDNA sequence has been obtained and deposited in the NCBI GenBank database. In phylogenetic analysis the specimen studied formed a separate clade among other individuals of B. phalloides with different geographical origins.

Conclusion

Conclusion. The new B. phalloides finding recorded in Dagestan allowed us to expand the knowledge on its ecology and distribution, as well as on intraspecific phylogenetic structure of the species red‐listed in the region and little‐known in the Caucasus.

биоразнообразиеДНК‐штрихкодированиеохрана грибовгастеромицетымакромицетыСеверо‐Восточный КавказфилогенияКрасная книгабаттаррея весёлковаяBiodiversityDNA barcodingfungal conservationgasteromycetesNorthEastern CaucasusphylogenyRed Data Booksandy stiltballАвторы благодарят к.б.н. А.Б. Исмаилова (Горный ботанический сад ДФИЦ РАН, Махачкала) за помощь в организации полевых исследований. Работа выполнена в рамках государственного задания БИН РАН по теме №122011900032‐7 «Гербарные фонды БИН РАН (история, сохранение, изучение и пополнение)» с использованием оборудования Центра коллективного пользования научным оборудованием «Клеточные и молекулярные технологии изучения растений и грибов» Ботанического института им. В.Л. Комарова РАН (Санкт‐Петербург).The authors are grateful to Dr. A.B. Ismailov (Mountain Botanical Garden, DFRC RAS, Makhachkala) for help in the organisation of field studies. This research was carried out within the framework of the institutional research project of the Komarov Botanical Institute RAS (project no. 122011900032‐7, “Herbarium funds of the BIN RAS (history, preservation, study and enrichment)”) using the equipment of the Core Facility Centre “Cell and Molecular Technologies in Plant Science” at the Komarov Botanical Institute, RAS (St. Petersburg, Russia).ReferencesBaldrian P., Valášková V. Degradation of cellulose by basidiomycetous fungi // FEMS Microbiology Reviews. 2008. V. 32. N 3. P. 501–521. https://doi.org/10.1111/j.1574‐6976.2008.00106.x. Baldrian P., Valášková V. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiology Reviews, 2008, vol. 32, no. 3, pp. 501–521. https://doi.org/10.1111/j.1574‐6976.2008.00106.xМухин В.А., Воронин П.Ю. Микогенное разложение древесины и эмиссия углерода в лесных экосистемах // Экология. 2007. N 1. С. 24–29.Mukhin V.A., Voronin P.Yu. Mycogenic decomposition of wood and carbon emission in forest ecosystems. Russian Journal of Ecology, 2007, vol. 38, no. 1, pp. 22–26.Казарцев И.А., Рощин В.И., Соловьев В.А. Разложение углеводов древесины Populus tremula и Picea abies под действием лигнинразрушающих грибов // Микология и фитопатология. 2014. Т. 48. N 2. С. 112–117.Kazartsev I.A., Roshchin V.I., Solovyev V.A. Degradation of Populus tremula and Picea abies wood carbohydrates by lignin‐degrading fungi. Mikologiya i fitopatologiya [Mikologiya i fitopatologiya]. 2014, vol. 48, no. 2, pp. 112–117. (In Russian)Boddy L., Frankland J.C., van West P. Ecology of saprotrophic basidiomycetes. London, Elsevier Academic Press, 2008, 372 p.Boddy L., Frankland J.C., van West P. Ecology of saprotrophic basidiomycetes. London, Elsevier Academic Press, 2008, 372 p.Bahram M., Netherway T. Fungi as mediators linking organisms and ecosystems // FEMS Microbiology Reviews. 2022. V. 46. N 2. Article id: fuab058. https://doi.org/10.1093/femsre/fuab058Bahram M., Netherway T. Fungi as mediators linking organisms and ecosystems. FEMS Microbiology Reviews, 2022, vol. 46, no. 2, article id: fuab058. https://doi.org/10.1093/femsre/fuab058Geml J., Leal C.M., Nagy R., Sulyok J. Abiotic environmental factors drive the diversity, compositional dynamics and habitat preference of ectomycorrhizal fungi in Pannonian forest types // Frontiers in Microbiology. 2022. V. 13. Art. 1007935. https://doi.org/10.3389/fmicb.2022.1007935Geml J., Leal C.M., Nagy R., Sulyok J. Abiotic environmental factors drive the diversity, compositional dynamics and habitat preference of ectomycorrhizal fungi in Pannonian forest types. Frontiers in Microbiology, 2022, vol. 13, art. 1007935. https://doi.org/10.3389/fmicb.2022.1007935Mueller G.M., Cunha K.M., May T.W., Allen J.L., Westrip J.R.S., Canteiro C., Costa‐Rezende D.H., Drechsler‐Santos E.R., Vasco‐Palacios A.M., Ainsworth A.M., Alves‐Silva G., Bungartz F., Chandler A., Gonçalves S.C., Krisai‐Greilhuber I., Iršėnaitė R., Jordal J.B., Kosmann T., Lendemer J., McMullin R.T., Mešić A., Motato‐Vásquez V., Ohmura Y., Næsborg R.R., Perini C., Saar I., Simijaca D., Yahr R., Dahlberg A. What do the first 597 Global Fungal Red List assessments tell us about the threat status of fungi? // Diversity. 2022. V. 14. N 9. Art. 736. https://doi.org/10.3390/d14090736Mueller G.M., Cunha K.M., May T.W., Allen J.L., Westrip J.R.S., Canteiro C., Costa‐Rezende D.H., Drechsler‐Santos E.R., Vasco‐Palacios A.M., Ainsworth A.M., Alves‐Silva G., Bungartz F., Chandler A., Gonçalves S.C., Krisai‐Greilhuber I., Iršėnaitė R., Jordal J.B., Kosmann T., Lendemer J., McMullin R.T., Mešić A., Motato‐Vásquez V., Ohmura Y., Næsborg R.R., Perini C., Saar I., Simijaca D., Yahr R., Dahlberg A. What do the first 597 Global Fungal Red List assessments tell us about the threat status of fungi? Diversity, 2022, vol. 14, no. 9, art. 736. https://doi.org/10.3390/d14090736Mace G.M., Collar N.J., Gaston K.J., Hilton‐Taylor C., Akçakaya H.R., Leader‐Williams N., Milner‐Gulland E.J., Stuart S.N. Quantification of extinction risk: IUCN’s system for classifying threatened species // Conservation Biology. 2008. V. 22. N 6. P. 1424–1442. https://doi.org/10.1111/j.1523‐1739.2008.01044.xMace G.M., Collar N.J., Gaston K.J., Hilton‐Taylor C., Akçakaya H.R., Leader‐Williams N., Milner‐Gulland E.J., Stuart S.N. Quantification of extinction risk: IUCN’s system for classifying threatened species. Conservation Biology, 2008, vol. 22, no. 6, pp. 1424–1442. https://doi.org/10.1111/j.1523‐1739.2008.01044.xDahlberg A., Genney D.R., Heilmann‐Clausen J. Developing a comprehensive strategy for fungal conservation in Europe: current status and future needs // Fungal Ecology. 2010. V. 3. N 2. P. 50–64. https://doi.org/10.1016/j.funeco.2009.10.004Dahlberg A., Genney D.R., Heilmann‐Clausen J. Developing a comprehensive strategy for fungal conservation in Europe: current status and future needs. Fungal Ecology, 2010, vol. 3, no. 2, pp. 50–64. https://doi.org/10.1016/j.funeco.2009.10.004Красная книга Республики Дагестан. Махачкала: Типография ИП Джамалудинов М.А., 2020. 800 с.Krasnaya kniga Respubliki Dagestan [Red Data Book of the Republic of Dagestan]. Makhachkala, Printing house of IE M.A. Jamaludinov, 2020, 800 p. (In Russian)Pegler D.N., Læssøe T., Spooner B.M. British puffballs, earthstars and stinkhorns. Kew, 1995, 255 p.Pegler D.N., Læssøe T., Spooner B.M. British puffballs, earthstars and stinkhorns. Kew, 1995, 255 p.Volobuev S., Shakhova N. Towards the discovery of active lignocellulolytic enzyme producers: a screening study of xylotrophic macrofungi from the Central Russian Upland // Iranian Journal of Science and Technology, Transactions A: Science. 2022. V. 46. N 1. P. 91–100. https://doi.org/10.1007/s40995‐021‐01245‐7Volobuev S., Shakhova N. Towards the discovery of active lignocellulolytic enzyme producers: a screening study of xylotrophic macrofungi from the Central Russian Upland. Iranian Journal of Science and Technology, Transactions A: Science, 2022, vol. 46, no. 1, pp. 91–100. https://doi.org/10.1007/s40995‐021‐01245‐7Benson D.A., Cavanaugh M., Clark K., Karsch‐Mizrachi I., Lipman D.J., Ostell J., Eric W. Sayers E.W. GenBank // Nucleic Acids Research. 2013. V. 41. N D1. P. D36–D42. https://doi.org/10.1093/nar/gks1195Benson D.A., Cavanaugh M., Clark K., Karsch‐Mizrachi I., Lipman D.J., Ostell J., Eric W. Sayers E.W. GenBank. Nucleic Acids Research, 2013, vol. 41, no. D1, pp. D36–D42. https://doi.org/10.1093/nar/gks1195Katoh K., Standley D.M. MAFFT multiple sequence alignment software version 7: improvements in performance and usability // Molecular Biology and Evolution. 2013. V. 30. N 4. P. 772–780. https://doi.org/10.1093/molbev/mst010Katoh K., Standley D.M. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 2013, vol. 30, no. 4, pp. 772–780. https://doi.org/10.1093/molbev/mst010Katoh K., Rozewicki J., Yamada K.D. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization // Briefings in Bioinformatics. 2019. V. 20. N 4. P. 1160–1166. https://doi.org/10.1093/bib/bbx108Katoh K., Rozewicki J., Yamada K.D. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 2019, vol. 20, no. 4, pp. 1160–1166. https://doi.org/10.1093/bib/bbx108Trifinopoulos J., Nguyen L.T., von Haeseler A., Minh B.Q. W‐IQ‐TREE: a fast online phylogenetic tool for maximum likelihood analysis // Nucleic Acids Research. 2016. V. 44. N W1. P. W232–W 235. https://doi.org/10.1093/nar/gkw256Trifinopoulos J., Nguyen L.T., von Haeseler A., Minh B.Q. W‐IQ‐TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 2016, vol. 44, no. W1, pp. W232–W 235. https://doi.org/10.1093/nar/gkw256Martín M.P., Johannesson H. Battarea phalloides and B. stevenii, insight into a long‐standing taxonomic puzzle // Mycotaxon. 2000. V. 76. P. 67–75.Martín M.P., Johannesson H. Battarea phalloides and B. stevenii, insight into a long‐standing taxonomic puzzle. Mycotaxon, 2000, vol. 76, pp. 67–75.Martín M.P., Rusevska K., Dueñas M., Karadelev M. Battarrea phalloides in Macedonia: genetic variability, distribution and ecology // Acta Mycologica. 2013. V. 48. N 1. P. 113–122. https://doi.org/10.5586/am.2013.013Martín M.P., Rusevska K., Dueñas M., Karadelev M. Battarrea phalloides in Macedonia: genetic variability, distribution and ecology. Acta Mycologica, 2013, vol. 48, no. 1, pp. 113–122. https://doi.org/10.5586/am.2013.013Garrido‐Benavent I. The Battarrea phalloides‐stevenii complex: multiple sources of evidence as a strategy to unveil cryptic species within poorly characterized taxa // Butlletí Societat Micològica Valenciana. 2014. V. 19. P. 17–35.Garrido‐Benavent I. The Battarrea phalloides‐stevenii complex: multiple sources of evidence as a strategy to unveil cryptic species within poorly characterized taxa. Butlletí Societat Micològica Valenciana, 2014, vol. 19, pp. 17–35.Shepherd L.D., Cooper J.A. First record of the fungus Battarrea phalloides (Agaricaceae) in New Zealand // New Zealand Journal of Botany. 2018. V. 56. N 1. P. 109–114. http://dx.doi.org/10.1080/0028825X.2017.1385491Shepherd L.D., Cooper J.A. First record of the fungus Battarrea phalloides (Agaricaceae) in New Zealand. New Zealand Journal of Botany, 2018, vol. 56, no. 1, pp. 109–114. http://dx.doi.org/10.1080/0028825X.2017.1385491iNaturalist contributors, iNaturalist (2022). iNaturalist Research‐grade Observations. iNaturalist.org. Occurrence dataset https://doi.org/10.15468/ab3s5x2022‐12‐20. URL: https://www.gbif.org/occurrence/4034748523 (дата обращения: 20.12.2022)iNaturalist contributors, iNaturalist (2022). iNaturalist Research‐grade Observations. iNaturalist.org. Occurrence dataset https://doi.org/10.15468/ab3s5x accessed via GBIF.org. Available at: https://www.gbif.org/occurrence/4034748523 (accessed 20.12.2022)Pilát A. (ed.) Gasteromycetes. Flora ČSR. B. 1. Praha, 1958, 836 p.Pilát A. (ed.) Gasteromycetes. Flora ČSR. B. 1. Praha, 1958, 836 p.Ivančević B, Mešić A, Tkalčec Z, Kušan I, Horjan I. Studies on Croatian Basidiomycota 3: the first record of Battarrea phalloides (Agaricales) with a worldwide taxonomic review of Battarrea species // Nova Hedwigia. 2016. V. 102. N 1–2. P. 197–209. https://doi.org/10.1127/nova_hedwigia/2015/0300Ivančević B., Mešić A., Tkalčec Z., Kušan I., Horjan I. Studies on Croatian Basidiomycota 3: the first record of Battarrea phalloides (Agaricales) with a worldwide taxonomic review of Battarrea species. Nova Hedwigia, 2016, vol. 102, no. 1–2, pp. 197–209. https://doi.org/10.1127/nova_hedwigia/2015/0300Akata İ., Altuntaş D., Sahin E., Alli H., Kabaktepe Ş. A note on Battarrea phalloides in Turkey // Mantar Dergisi. 2021. V. 12. N 1. P. 1–9. https://doi.org/10.30708.mantar.800585Akata İ., Altuntaş D., Sahin E., Alli H., Kabaktepe Ş. A note on Battarrea phalloides in Turkey. Mantar Dergisi, 2021, vol. 12, no. 1, pp. 1–9. https://doi.org/10.30708.mantar.800585Fraiture A., Otto P. Distribution, ecology and status of 51 macromycetes in Europe. Results of the ECCF Mapping Programme // Scripta Botanica Belgica. 2015. V. 53. P. 1–247.Fraiture A., Otto P. Distribution, ecology and status of 51 macromycetes in Europe. Results of the ECCF Mapping Programme. Scripta Botanica Belgica, 2015, vol. 53, pp. 1–247.Battarrea phalloides. URL: https://redlist.info/iucn/species_view/159853/ (дата обращения: 16.12.2022)Battarrea phalloides. Available at: https://redlist.info/iucn/species_view/159853/ (accessed 16.12.2022)Красная книга Республики Калмыкия. В 2‐х томах. Том 2. Редкие и находящиеся под угрозой исчезновения растения и грибы. Элиста: ЗАОр «НПП «Джангар», 2014. 199 с.Krasnaya kniga Respubliki Kalmykiya. V 2‐kh tomakh. Tom 2. Redkie i nakhodyashchiesya pod ugrozoi ischeznoveniya rasteniya i griby [Red Data Book of the Republic of Kalmykia. In 2 volumes. Vol. 2. Rare and endangered plants and fungi]. Elista, Dzhangar Publ., 2014, 199 p. (In Russian)Gargano M.L., Venturella G., Ferraro V. Is Battarrea phalloides really an endangered species? // Plant Biosystems – An International Journal Dealing with all Aspects of Plant Biology. 2021. V. 155. N 4. P. 759–762. https://doi.org/10.1080/11263504.2020.1779847Gargano M.L., Venturella G., Ferraro V. Is Battarrea phalloides really an endangered species? Plant Biosystems – An International Journal Dealing with all Aspects of Plant Biology, 2021, vol. 155, no. 4, pp. 759–762. https://doi.org/10.1080/11263504.2020.1779847

The authors declare that there are no conflicts of interest present.