ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2023-1-62-81ecodag-2750Research ArticleЭКОЛОГИЯ МИКРООРГАНИЗМОВИсследование ингибирующей активности экстрактов, фракций и вторичных метаболитов Silene spp. (Caryophylaceae) и Serratula cupuliformis (Asteraceae) на репликацию коронавируса SARS-CoV-2Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophyllaceae) and Serratula cupuliformis (Asteraceae) on the replication of SARS-CoV-2 coronavirushttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.KazachinskaiaE. I.

Елена И. Казачинская - доктор биологических наук, ведущий научный сотрудник ФИЦ ФТМ СО РАН, ведущий научный сотрудник ФБУН ГНЦ ВБ Вектор.

630559 Новосибирская область, Новосибирск, р/п Кольцово 32-1. Тел. +79095307441

Elena I. Kazachinskaia - Doctor of Biology, Leading Researcher, Research Institute of Virology, FRC of Fundamental and Translational Medicine, Siberian Branch of RAS & Leading Researcher, Vector SRC of Virology and Biotechnology.

321 r/p Koltsovo Novosibirsk region, Novosibirsk, 630559. Tel. +79095307441

lena.kazachinskaia@mail.ruhttps://orcid.org/0000-0002-4417-8340ЗибареваЛ. Н.ZibarevaL. N.

Лариса Н. Зибарева

Томск

Larisa N. Zibareva

Tomsk

https://orcid.org/0000-0002-4287-8327ФилоненкоЕ. С.FilonenkoE. S.

Томск

Elena S. Filonenko

Tomsk

https://orcid.org/0000-0001-9102-6756ИвановаА. В.IvanovaA. V.

Алла В. Иванова

Новосибирск

Alla V. Ivanova

Novosibirsk

https://orcid.org/0009-0004-1940-3253ГаджиеваМ. М.GadzhievaM. M.

Маликат М. Гаджиева

Москва

Malikat M. Gadzhieva

Moscow

https://orcid.org/0000-0003-0147-1341БекшоковК. К.BekshokovK. K.

Казбек К. Бекшоков

Москва

Kazbek K. Bekshokov

Moscow

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.KononovaYu. V.

Юлия В. Кононова

Новосибирск

Yulia V. Kononova

Novosibirsk

https://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovA. A.

Александр А. Чепурнов

Новосибирск

Alexander А. Chepurnov

Novosibirsk

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovA. М.

Александр М. Шестопалов

Новосибирск, Махачкала

Alexander М. Shestopalov

Novosibirsk, Makhachkala

Научно-исследовательский институт вирусологии, Федеральный исследовательский центр фундаментальной и трансляционной медицины, Министерство науки и высшего образования Российской Федерации; Государственный научный центр вирусологии и биотехнологии ВекторResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Vector State Research Centre of Virology and BiotechnologyНациональный исследовательский Томский государственный университетNational Research Tomsk State UniversityГосударственный научный центр вирусологии и биотехнологии ВекторVector State Research Centre of Virology and BiotechnologyРоссийский национальный исследовательский медицинский университет им. Н.И. ПироговаN.I. Prigorov Russian National Research Medicine UniversityПервый Московский государственный медицинский университет имени И.М. Сеченова, Министерство здравоохранения Российской ФедерацииI.M. Sechenov First Moscow State Medical University, Russian Ministry of HealthНаучно-исследовательский институт вирусологии, Федеральный исследовательский центр фундаментальной и трансляционной медицины, Министерство науки и высшего образования Российской ФедерацииResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesНаучно-исследовательский институт вирусологии, Федеральный исследовательский центр фундаментальной и трансляционной медицины, Министерство науки и высшего образования Российской ФедерацииResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Dagestan State Universityфундаментальной и трансляционной медицины, Министерство науки и высшего образования Российской Федерации; Дагестанский государственный университетResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Dagestan State University2023300320231816281Copyright © Казачинская Е.И., Зибарева Л.Н., Филоненко Е.С., Иванова А.В., Гаджиева М.М., Бекшоков К.К., Кононова Ю.В., Чепурнов А.А., Шестопалов А.М., 20232023Казачинская Е.И., Зибарева Л.Н., Филоненко Е.С., Иванова А.В., Гаджиева М.М., Бекшоков К.К., Кононова Ю.В., Чепурнов А.А., Шестопалов А.М.Kazachinskaia E.I., Zibareva L.N., Filonenko E.S., Ivanova A.V., Gadzhieva M.M., Bekshokov K.K., Kononova Y.V., Chepurnov A.A., Shestopalov A.М.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2750Цель

Цель. Анализ in vitro ингибирующей активности экстрактов, фракций и вторичных метаболитов растений рода Silene семейства Гвоздичные [Caryophylaceae) и Serratula cupuliformis [Asteraceae) на репликацию SARS-CoV-2.

Материалы и методы

Материалы и методы. Приготовлены этанольные экстракты и бутанольные фракции Silene spp. и Serratula cupuliformis, интродуцированных в Сибирском ботаническом саду ТГУ. Выделены флавоноид шафтозид и экдистероид 20-гидроксиэкдизон [20-Е) из Lychnis chalcedonica. Анализ БАВ выполнен методом ВЭЖХ. Анализ in vitro ингибирующей активности растительных препаратов на репликацию SARS-CoV-2 проводили в культуре клеток Vero методом прямой инактивации [нейтрализации) вирионов. Образцы сравнения - сухие этанольные экстракты чаги, специи гвоздики и корня солодки голой.

Результаты

Результаты. Выявлена ингибирующая активность этанольных экстрактов и бутанольных фракций Silene spp., а также индивидуальных соединений [шафтозида и 20-Е) в диапазоне 50%-ных эффективных концентраций [EC50) при растворении в воде от 339,85±83,92 мкг/мл до 1,59±0,39 мкг/мл и при растворении в ДМСО от 119,34±26,34 мкг/мл до 2,22±0,57 мкг/мл, соответственно. Бутанольная фракция Serratula cupuliformis была активна с EC50=21,74±4,80 и 27,42±6,05 мкг/мл. Такой результат для некоторых образцов Silene spp. и Serratula cupuliformis сопоставим со значениями EC50 препаратов сравнения.

Заключение

Заключение. Полученные результаты предполагают наличие в исследуемых растительных препаратах Silene spp. и Serratula cupuliformis БАВ, действующих деструктивно на вирионы SARS-CoV-2 и влияющих на один из основных этапов его «жизненного» цикла - на прикрепление к рецепторам чувствительных клеток.

Aim

Aim. In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene [Caryophylaceae] and Serratula cupuliformis [Asteraceae) on the replication of SARS-CoV-2.

Material and Methods

Material and Methods. Silene spp. and Serratula cupuliformis of the Siberian Botanical Garden of National Research Tomsk State University were used. Ethanol extracts and butanol fractions of Silene spp. were prepared. The flavonoid shaftoside and the ecdysteroid 20-hydroxyecdysone from Lychnis chalcedonica were isolated. Analysis of BAS was carried out by the HPLC method. In vitro analysis of the inhibitory activity of extracts on SARS-CoV-2 replication was performed in Vero cell culture by direct inactivation [neutralization) of virions. Comparison samples were dry ethanol extracts of chaga [Inonotus obliquus, Basidiomycota), spices of cloves [Syzygium aromaticum, Myrtaceae) and root of licorice [Glycyrrhiza glabra L., Fabaceae).

Results

Results. The inhibitory activity of ethanol extracts and butanol fractions of Silene spp., as well as individual compounds [shaftozide and 20-E) was revealed in the range of 50% effective concentrations [EC50) when dissolved in water from 339.85±83.92 pg/ml to 1.59±0.39 pg/ml and when dissolved in DMSO from 119.34±26.34 pg/ml to 2.22±0.57 pg/ml, respectively. The butanol fraction of Serratula cupuliformis was active with EC50=21.74±4.80 and 27.42±6.05 pg/mL. These results for some samples of Silene spp. and Serratula cupuliformis are comparable to the EC50 values of the comparators.

Conclusion

Conclusion. The results obtained suggest the presence of biologically active substances in the herbal preparations studied that act destructively on virions of SARS-CoV-2 and affect one of the main stages of its "life" cycle - on the attachment to receptors of sensitive cells.

SARS-CoV-2растительные препаратыингибирующая активностьSARS-CoV-2herbal preparationsinhibitory activityИсследование выполнено в рамках проектной части государственного задания Министерства науки и высшего образования Российской Федерации в сфере научной деятельности (проект № FSWM № МК-2021.0007) «Поиск перспективных растительных источников флавоноидов, выращивание растений, изучение состава и содержания вторичных метаболитов, получение комплексов, выделение индивидуальных соединений для анализа активности». Исследование выполнено за счет гранта Российского научного фонда No 22-24-00199, https://rscf.ru/project/22-24-00199The study was carried out within the framework of the project part of the state task of the Ministry of Science and Higher Education of the Russian Federation in the field of scientific activity (project no. FSWM no. MK-2021.0007) "Search for promising plant sources of flavonoids, plant cultivation, study of the composition and content of secondary metabolites, obtaining complexes, isolation of individual compounds for activity analysis". This study was funded by the RSF according to the research project No 22-24-00199, https://rscf.ru/project/22-24-00199ReferencesOwen L., Laird K., Shivkumar M. Antiviral plant-derived natural products to combat RNA viruses: Targets throughout the viral life cycle // Lett Appl Microbiol. 2022. V. 75. N 3. P. 476-499. DOI: 10.1111/lam.13637Owen L., Laird K., Shivkumar M. Antiviral plant-derived natural products to combat RNA viruses: Targets throughout the viral life cycle. LettApplMicrobiol., 2022, vol. 75, no. 3, pp. 476-499. DOI: 10.1111/lam.13637Pattnaik G.P., Chakraborty H. Entry Inhibitors: Efficient Means to Block Viral Infection // J Membr Biol. 2020. V. 253. N 5. P. 425444. DOI: 10.1007/s00232-020-00136-zPattnaik G.P., Chakraborty H. Entry Inhibitors: Efficient Means to Block Viral Infection. J Membr Biol., 2020, vol. 253, no. 5, pp. 425-444. DOI: 10.1007/s00232-020-00136-zBai L., Zhao Y., Dong J., Liang S., Guo M., Liu X., Wang X., Huang Z., Sun X., Zhang Z. et al. Coinfection with influenza A virus enhances SARS-CoV-2 infectivity // Cell Res. 2021. V. 31. N 4. P. 395-403. DOI: 10.1038/s41422-021-00473-1Bai L., Zhao Y., Dong J., Liang S., Guo M., Liu X., Wang X., Huang Z., Sun X., Zhang Z. et al. Coinfection with influenza A virus enhances SARS-CoV-2 infectivity. Cell Res., 2021, vol. 31, no. 4, pp. 395-403. DOI: 10.1038/s41422-021-00473-1Abdoli A., Falahi S., Kenarkoohi A. COVID-19-associated opportunistic infections: a snapshot on the current reports // Clin Exp Med. 2021. V. 22. N 3. P. 327-346. DOI: 10.1007/s10238-021-00751-7Abdoli A., Falahi S., Kenarkoohi A. COVID-19-associated opportunistic infections: a snapshot on the current reports. Clin Exp Med., 2021, vol. 22, no. 3, pp. 327-346. DOI: 10.1007/s10238-021-00751-7SeeRle J., Hippchen T., Schnitzler P., Gsenger J., Giese T., Merle U. High rate of HSV-1 reactivation in invasively ventilated COVID-19 patients: Immunological findings // PLoS ONE. 2021. V. 16. N 7:e0254129. DOI: 10.1371/journal.pone.0254129SeeRle J., Hippchen T., Schnitzler P., Gsenger J., Giese T., Merle U. High rate of HSV-1 reactivation in invasively ventilated COVID-19 patients: Immunological findings. PLoS ONE, 2021, vol. 16, no. 7:e0254129. DOI: 10.1371/journal.pone.0254129Mirzaei R., Goodarzi P., Asadi M., Soltani A., Aljanabi H.A.A., Jeda A.S., Dashtbin S., Jalalifar S., Mohammadzadeh R., Teimoori A. et al. Bacterial co-infections with SARS-CoV-2 // IUBMB Life. 2020. V. 72. N 10. P. 2097-2111. DOI: 10.1002/iub.2356Mirzaei R., Goodarzi P., Asadi M., Soltani A., Aljanabi H.A.A., Jeda A.S., Dashtbin S., Jalalifar S., Mohammadzadeh R., Teimoori A. et al. Bacterial co-infections with SARS-CoV-2. IUBMB Life, 2020, vol. 72, no. 10, pp. 2097-2111. DOI: 10.1002/iub.2356Zeng L., Watanabe N., Yang Z. Understanding the biosyntheses and stress response mechanisms of aroma compounds in tea (Camellia sinensis) to safely and effectively improve tea aroma // Crit. Rev. Food Sci. 2019. N 59. P. 2321-2334. DOI: 10.1080/10408398.2018.1506907Zeng L., Watanabe N., Yang Z. Understanding the biosyntheses and stress response mechanisms of aroma compounds in tea (Camellia sinensis) to safely and effectively improve tea aroma. Crit. Rev. Food Sci., 2019, no. 59, pp. 2321-2334. DOI: 10.1080/10408398.2018.1506907Zaynab M., Fatima M., Sharif Y., Zafar M.H., Ali H., Khan K.A. Role of primary metabolites in plant defense against pathogens // Microb Pathog. 2019. N 137:103728. DOI: 10.1016/j.micpath.2019.103728Zaynab M., Fatima M., Sharif Y., Zafar M.H., Ali H., Khan K.A. Role of primary metabolites in plant defense against pathogens. Microb Pathog., 2019, no. 137:103728. DOI: 10.1016/j.micpath.2019.103728Aanouz I., Belhassan A., El-Khatabi K., Lakhlifi T., El-Ldrissi M., Bouachrine M. Moroccan Medicinal plants as inhibitors against SARS-CoV-2 main protease: Computational investigations // J Biomol Struct Dyn. 2021. V. 39. N 8. P. 2971-2979. DOI: 10.1080/07391102.2020.1758790Aanouz I., Belhassan A., El-Khatabi K., Lakhlifi T., El-Ldrissi M., Bouachrine M. Moroccan Medicinal plants as inhibitors against SARS-CoV-2 main protease: Computational investigations. J BiomolStruct Dyn., 2021, vol. 39, no. 8, pp. 2971-2979. DOI: 10.1080/07391102.2020.1758790Adhikari B., Marasini B.P., Rayamajhee B., Bhattarai B.R., Lamichhane G., Khadayat K., Adhikari A., Khanal S. , Parajuli N. Potential roles of medicinal plants for the treatment of viral diseases focusing on COVID-19: A review // Phytother Res. 2021. V. 35. N 3. P. 1298-1312. DOI: 10.1002/ptr.6893Adhikari B., Marasini B.P., Rayamajhee B., Bhattarai B.R., Lamichhane G., Khadayat K., Adhikari A., Khanal S., Parajuli N. Potential roles of medicinal plants for the treatment of viral diseases focusing on COVID-19: A review. Phytother Res, 2021, vol. 35, no. 3, pp. 1298-1312. DOI: 10.1002/ptr.6893Mohamed F.F., Anhlan D., Schofbanker M., Schreiber A., Nica Classen, Hensel A., Hempel G., Scholz W., Kuhn J., Hrincius E.R., Ludwig S. Hypericum perforatum and Its Ingredients Hypericin and Pseudohypericin Demonstrate an Antiviral Activity against SARS-CoV-2 // Pharmaceuticals (Basel). 2022. V. 15. N 5:530. DOI: 10.3390/ph15050530Mohamed F.F., Anhlan D., Schofbanker M., Schreiber A., Nica Classen 3, Hensel A., Hempel G., Scholz W., Kuhn J., Hrincius E.R., Ludwig S. Hypericum perforatum and Its Ingredients Hypericin and Pseudohypericin Demonstrate an Antiviral Activity against SARS-CoV-2. Pharmaceuticals (Basel), 2022, vol. 15, no. 5:530. DOI: 10.3390/ph15050530Satish C., Rawat D.S. Medicinal plants of the family Caryophyllaceae: a review of ethno-medicinal uses and pharmacological properties // Integr Med Res. 2015. V. 4. N 3. P. 123-131. DOI: 10.1016/j.imr.2015.06.004Satish C., Rawat D.S. Medicinal plants of the family Caryophyllaceae: a review of ethno-medicinal uses and pharmacological properties. Integr Med Res, 2015, vol. 4, no. 3, pp. 123-131. DOI: 10.1016/j.imr.2015.06.004Mamadalieva N.Z., Lafont R., Wink M. Diversity of secondary metabolites in the genus Silene L. (Caryophyllaceae) – structures distribution, and biological properties // Diversity. 2014. N 6. P. 415-499.Mamadalieva N.Z., Lafont R., Wink M. Diversity of secondary metabolites in the genus Silene L. (Caryophyllaceae) - structures distribution, and biological properties. Diversity, 2014, no. 6, pp. 415-499.Orhan I., Deliorman-Orhan D., Ozcelik B. Antiviral activity and cytotoxicity of the lipophilic extracts of various edible plants and their fatty acids // Food Chem. 2009. N 115. P. 701-705.Orhan I., Deliorman-Orhan D., Ozcelik B. Antiviral activity and cytotoxicity of the lipophilic extracts of various edible plants and their fatty acids. Food Chem., 2009, no. 115, pp. 701-705.Zibareva L.N., Zueva E.P., Razina T.G., Amosova E.N., Krylova S. G., Lopatina K.A., Rybalkina O.Y., Badulina A.A., Safonova E.A., Babushkina M.S., Filonenko E.S., Galiulina A.V. The effect of Lychnis chalcedonica L. flavonoids on the development of tumors in mice and the effectiveness of treatment with cyclophosphamide // AIP Conf. Proc. 2015. 1688, 030031. DOI: 10.1063/1.4936026Zibareva L.N., Zueva E.P., Razina T.G., Amosova E.N., Krylova S.G., Lopatina K.A., Rybalkina O.Y., Badulina A.A., Safonova E.A., Babushkina M.S., Filonenko E.S., Galiulina A.V. The effect of Lychnis chalcedonica L. flavonoids on the development of tumors in mice and the effectiveness of treatment with cyclophosphamide. AIP Conf. Proc. 2015, vol. 1688, 030031. DOI: 10.1063/1.4936026Amosova E.N., Zueva E.P., Lopatina K.A., Safonova E.A., Razina T. G., Rubalkina O.Yu., Zibareva L.N. Influence of Lychnis chalcedonica L. flavonoids on transplanted tumor development and cytostatic therapy effectiveness in mice // Pharmaceutical Chemistry Journal. 2019. V. 53. N 5. P. 458-461. DOI: 10.1007/s11094-019-02019-7Amosova E.N., Zueva E.P., Lopatina K.A., Safonova E.A., Razina T.G., Rubalkina O.Yu., Zibareva L.N. Influence of Lychnis chalcedonica L. flavonoids on transplanted tumor development and cytostatic therapy effectiveness in mice. Pharmaceutical Chemistry Journal, 2019, vol. 53, no. 5, pp. 458-461. DOI: 10.1007/s11094-019-02019-7Krylova S.G., Zueva E.P., Zibareva L.N., Amosova E.N., Razina T.G. Antiulcer activity of extracts of ecdysteroid-containing plants of genera Lychnis and Silene of the Caryophyllaceae family // Bull Exp Biol Med. 2014. V. 158. N 2. P. 225-228. DOI: 10.1007/s10517-014-2728-1Krylova S.G., Zueva E.P., Zibareva L.N., Amosova E.N., Razina T.G. Antiulcer activity of extracts of ecdysteroid-containing plants of genera Lychnis and Silene of the Caryophyllaceae family. Bull Exp Biol Med., 2014, vol. 158, no. 2, pp. 225-228. DOI: 10.1007/s10517-014-2728-1Nesterova Yu.V., Povet'eva T.N., Zibareva L.N., Suslov N.I., Zueva E.P., Aksinenko S.G., Afanas'eva O.G., Krylova S.G., Amosova E.N., Rybalkina O.Yu., Lopatina K.A. Anti-Inflammatory and Analgesic Activities of the Complex of Flavonoids from Lychnis chalcedonica L. // Bull Exp Biol Med. 2017. V. 163. N 2. P. 222-225. DOI: 10.1007/s10517-017-3771-5Nesterova Yu.V., Povet'eva T.N., Zibareva L.N., Suslov N.I., Zueva E.P., Aksinenko S.G., Afanas'eva O.G., Krylova S.G., Amosova E.N., Rybalkina O.Yu., Lopatina K.A. Anti-Inflammatory and Analgesic Activities of the Complex of Flavonoids from Lychnis chalcedonica L. Bull Exp Biol Med., 2017, vol. 163, no. 2, pp. 222225. DOI: 10.1007/s10517-017-3771-5Plotnikov M.B., Zibareva L.N., Vasil'ev A.S., Aliev O.I., Anishchenko A.M., Maslov M.Yu. Antihyperglycaemic, haemorheological and antioxidant activities of Lychnis chalcedonica L. extract in a streptozotocin-induced rat model of diabetes mellitus // J Complement Integr Med. 2019. V. 17. N 2. P. 20170028. DOI: 10.1515/jcim-2017-0028Plotnikov M.B., Zibareva L.N., Vasil'ev A.S., Aliev O.I., Anishchenko A.M., Maslov M.Yu. Antihyperglycaemic, haemorheological and antioxidant activities of Lychnis chalcedonica L. extract in a streptozotocin-induced rat model of diabetes mellitus. J Complement Integr Med, 2019, vol. 17, no. 2, pp. 20170028. DOI: 10.1515/jcim-2017-0028Shen N., Wang T., Gan Q., Liu S., Wang L., Jin B. Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity // Food Chem. 2022. N 383. Article ID: 132531. DOI: 10.1016/j.foodchem.2022.132531Shen N., Wang T., Gan Q., Liu S., Wang L., Jin B. Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chem., 2022, no. 383, article id: 132531. DOI: 10.1016/j.foodchem.2022.132531Zakaryan H., Arabyan E., Oo A.,Zandi K. Flavonoids: promising natural compounds against viral infections // Arch Virol. 2017. V. 162. N 9. P. 2539-2551. DOI: 10.1007/s00705-017-3417-yZakaryan H., Arabyan E., Oo A., Zandi K. Flavonoids: promising natural compounds against viral infections. Arch Virol, 2017, vol. 162, no. 9, pp. 2539-2551. DOI: 10.1007/s00705-017-3417-yJo S., KimS., Shin D.H., Kim M.-S. Inhibition of SARS-CoV 3CL protease by flavonoids // J Enzyme Inhib Med Chem. 2020. V. 35. N 1. P. 145-151. DOI: 10.1080/14756366.2019.1690480Jo S., Kim S., Shin D.H., Kim M.-S. Inhibition of SARS-CoV 3CL protease by flavonoids. J Enzyme Inhib Med Chem, 2020, vol. 35, no. 1, pp. 145-151. DOI: 10.1080/14756366.2019.1690480Mhatre S., Srivastava T., Naik S., Patravale V. Antiviral activity of green tea and black tea polyphenols in prophylaxis and treatment of COVID-19: A review // Phytomedicine. 2021. N 85:153286. DOI: 10.1016/j.phymed.2020.153286Mhatre S' , Srivastava T., Naik S., Patravale V. Antiviral activity of green tea and black tea polyphenols in prophylaxis and treatment of COVID-19: A review. Phytomedicine, 2021, no. 85, article id: 153286. DOI: 10.1016/j.phymed.2020.153286Tarbeeva D.V., Krylova N.V., Iunikhina O.V., Likhatskaya G.N., Kalinovskiy A.I., Grigorchuk V.P., Shchelkanov M.Y., Fedoreyev S.A. Biologically active polyphenolic compounds from Lespedeza bicolor // Fitoterapia. 2022. N 157:105121. DOI: 10.1016/j.fitote.2021.105121Tarbeeva D.V., Krylova N.V., Iunikhina O.V., Likhatskaya G.N., Kalinovskiy A.I., Grigorchuk V.P., Shchelkanov M.Y., Fedoreyev S.A. Biologically active polyphenolic compounds from Lespedeza bicolor. Fitoterapia, 2022, no. 157, article id: 105121. DOI: 10.1016/j.fitote.2021.105121Zhang H., Li Z., Li C., Chen R., Liu T., Jiang Y. Antiviral Effect of Polyphenolic Substances in Geranium wilfordii Maxim against HSV-2 Infection Using in vitro and in silico Approaches Evid Based Complement // Alternat Med. 2022. N 2022:7953728. DOI: 10.1155/2022/7953728Zhang H., Li Z., Li C., Chen R., Liu T., Jiang Y. Antiviral Effect of Polyphenolic Substances in Geranium wilfordii Maxim against HSV-2 Infection Using in vitro and in silico Approaches Evid Based Complement. Alternat Med., 2022, no. 2022, article id: 7953728. DOI: 10.1155/2022/7953728Saadh M.J., Jaber S.A., Alaraj M., Alafnan A. Apigenin inhibits infectious bronchitis virus replication in ovo // Eur Rev Med Pharmacol Sci. 2022. V. 26. N 15. P. 5367-5371. DOI: 10.26355/eurrev_202208_29403Saadh M.J., Jaber S.A., Alaraj M., Alafnan A. Apigenin inhibits infectious bronchitis virus replication in ovo. Eur Rev Med PharmacolSci, 2022, vol. 26, no. 15, pp. 5367-5371. DOI: 10.26355/eurrev_202208_29403Wang S.-C., Chou I.-W., Hung M.-C. Natural tannins as anti-SARS-CoV-2 compounds // Int J Biol Sci. 2022. V. 18. N 12:46694676. DOI: 10.7150/ijbs.74676Wang S.-C., Chou I.-W., Hung M.-C. Natural tannins as anti-SARS-CoV-2 compounds. Int J Biol Sci., 2022, vol. 18, no. 12, article id: 4669-4676. DOI: 10.7150/ijbs.74676Liu H., Ye F., Sun Q., Liang H., Li C., Li S., Lu R., Huang B., Tan W., Lai L. Scutellaria baicalensis extract and baicalein inhibit replication of SARS-CoV-2 and its 3C-like protease in vitro CoV-2 // J Enzyme Inhib Med Chem. 2021. V. 36. N 1. P. 497-503. DOI: 10.1080/14756366.2021.1873977Liu H., Ye F., Sun Q., Liang H., Li C., Li S., Lu R., Huang B., Tan W., Lai L. Scutellaria baicalensis extract and baicalein inhibit replication of SARS-CoV-2 and its 3C-like protease in vitro CoV-2. J Enzyme Inhib Med Chem., 2021, vol. 36, no. 1, pp. 497-503. DOI: 10.1080/14756366.2021.1873977Song J., Zhang L., Xu Y., Yang D., Zhang L., Yang S., Zhang W., Wang J., Tian S., Yang S., Yuan T., Liu A., Lv Q., Li F. , Liu H., Hou B., Peng X., Lu Y., Du G. The comprehensive study on the therapeutic effects of baicalein for the treatment of COVID-19 in vivo and in vitro // Biochem Pharmacol. 2021. N 183:114302. DOI: 10.1016/j.bcp.2020.114302Song J., Zhang L., Xu Y., Yang D., Zhang L., Yang S., Zhang W., Wang J., Tian S., Yang S., Yuan T., Liu A., Lv Q., Li F. , Liu H., Hou B., Peng X., Lu Y., Du G. The comprehensive study on the therapeutic effects of baicalein for the treatment of COVID-19 in vivo and in vitro. Biochem Pharmacol., 2021, no. 183, article id:114302. DOI: 10.1016/j.bcp.2020.114302Yi Y., Zhang M., Xue H., Yu R., Bao Y.-O., Kuang Y., Chai Y., Ma W., Wang J., Shi X. et al.. Schaftoside inhibits 3CLpro and PLpro of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19 // Acta Pharm Sin B. 2022. DOI: 10.1016/j.apsb.2022.07.017Yi Y., Zhang M., Xue H., Yu R., Bao Y.-O., Kuang Y., Chai Y., Ma W., Wang J., Shi X. et al. Schaftoside inhibits 3CLpro and PLpro of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19. Acta Pharm Sin B, 2022, vol. 12, no. 11, pp. 4154-4164. DOI: 10.1016/j.apsb.2022.07.017Arif Y., Singh P., Bajguz A., Hayat S. Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones // Int J Mol Sci. 2022. V. 23. N 15. P. 8664. DOI: 10.3390/ijms23158664Arif Y., Singh P., Bajguz A., Hayat S. Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones. Int J Mol Sci., 2022, vol. 23, no. 15, pp. 8664. DOI: 10.3390/ijms23158664Dinan L., Dioh W., Veillet S., Lafont R. 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases // Biomedicines. 2021. V. 9. N 5. P. 492. DOI: 10.3390/biomedicines9050492Dinan L., Dioh W., Veillet S., Lafont R. 20-Hydroxyecdysone, from Plant Extracts to Clinical Use: Therapeutic Potential for the Treatment of Neuromuscular, Cardio-Metabolic and Respiratory Diseases. Biomedicines, 2021, vol. 9, no. 5, pp. 492. DOI: 10.3390/biomedicines9050492Dioh W., Chabane M., Tourette C., Azbekyan A., Morelot-Panzini C., Hajjar L.A., Lins M., Nair G.B., Whitehouse T., Mariani J., Latil M., Camelo S., Lafont R., Dilda P.J., Veillet S., Agus S. Testing the efficacy and safety of BIO101, for the prevention of respiratory deterioration, in patients with COVID-19 pneumonia (COVA study): a structured summary of a study protocol for a randomised controlled trial // Trials. 2021. V. 22. N 1. P. 42. DOI: 10.1186/s13063-020-04998-5Dioh W., Chabane M., Tourette C., Azbekyan A., Morelot-Panzini C., Hajjar L.A., Lins M., Nair G.B., Whitehouse T., Mariani J., Latil M., Camelo S., Lafont R., Dilda P.J., Veillet S., Agus S. Testing the efficacy and safety of BIO101, for the prevention of respiratory deterioration, in patients with COVID-19 pneumonia (COVA study): a structured summary of a study protocol for a randomised controlled trial. Trials, 2021, vol. 22, no. 1, pp. 42. DOI: 10.1186/s13063-020-04998-5Hussain A. A phylogenetic perspective of antiviral species of the genus Artemisia (Asteraceae - Anthemideae): A proposal of anti SARS-CoV-2 (COVID-19) candidate taxa // J Herb Med. 2022. N 36:100601. DOI: 10.1016/j.hermed.2022.100601Hussain A. A phylogenetic perspective of antiviral species of the genus Artemisia (Asteraceae - Anthemideae): A proposal of anti SARS-CoV-2 (COVID-19) candidate taxa. J Herb Med., 2022, no. 36, article id: 100601. DOI: 10.1016/j.hermed.2022.100601Казачинская Е.И., Романова В.Д., Иванова А.В., Чепурнов А.А., Кононова Ю.В., Шауло Д.Н., Романюк В.В., Шестопалов А.М. Ингибирующая активность сухих этанольных экстрактов Artemisia spp. на репликацию SARS-CoV-2 in vitro // Юг России: экология, развитие. 2022. Т. 17. N 4. С. 111-129. DOI: 10.18470/1992-1098-2-22-4-111-129Kazachinskaia E.I., Romanova V.D., Ivanova A.V., Chepurnov А.А., Kononova Y.V., Shaulo D.N., Romanyuk V.V., Shestopalov А.М. Inhibitory activity of dry ethanol extracts of Artemisia spp. on SARS-CoV-2 replication in vitro. South of Russia: ecology, development, 2022, vol. 17, no. 4, pp. 111-129. (In Russian) DOI: 10.18470/1992-1098-2-22-4-111-129Зибарева Л.Н., Еремина В.И. Способ увеличения степени извлечения экдистероидов из растительных объектов // Пат. РФ № 2472519C1; опубл. 20.01.2013 в Бюл. N 2.Zibareva L.N., Yeriomina V.I. Sposob uvelicheniya stepeni izvlecheniya ekdisteroidov iz rastitel'nykh ob "ektov [A method for increasing the degree of extraction of ecdysteroids from plant objects]. Patent of the Russian Federation no. 2472519C1 published in Bulletin of Inventions no. 2. 20.01.2013. (In Russian)Zibareva L., Athipornchai A., Wonganan O., Suksamrarn A. Application of ultrasound to extraction of biologically active substances of some Serratula species // International Journal of Food and Biosystems Engineering. 2017. V. 5. N 1. P. 31-37. URL: http://fabe.gr/en/journal/journal (дата обращения: 12.12.2022)Zibareva L., Athipornchai A., Wonganan O., Suksamrarn A. Application of ultrasound to extraction of biologically active substances of some Serratula species. International Journal of Food and Biosystems Engineering. 2017, vol. 5, no. 1, pp. 31-37. Available at: http://fabe.gr/en/journal/journal (accessed 12.12.2022)Zibareva L., Yeriomina V.I., Munkhjargal N., Girault J.-P., Dinan L., Lafont R. The Phytoecdysteroid Profiles of 7 Species of Silene (Caryophyllaceae) // Archives of insect biochemistry and physiology. 2009. V. 72. N 4. P. 234-248. DOI: 10.1002/arch.20331Zibareva L., Yeriomina V.I., Munkhjargal N., Girault J.-P., Dinan L., Lafont R. The Phytoecdysteroid Profiles of 7 Species of Silene (Caryophyllaceae). Archives of insect biochemistry and physiology, 2009, vol. 72, no. 4, pp. 234-248. DOI: 10.1002/arch.20331Зибарева Л.Н., Филоненко Е.С., Черняк Е.И., Морозов С.В., Котельников О.А. Флавоноиды некоторых видов растений рода Silene // Химия растительного сырья. 2022. N 3. С. 109118. DOI: 10.14258/jcprm.20220310592Zibareva L.N., Filonenko E.S., Chernyak E.I., Morozov S.V., Kotelnikov O.A. Flavonoids of some plant species of the genus Silene. Chemistry of plant raw materials, 2022, no. 3, pp. 109-118. (In Russian) DOI: 10.14258/jcprm.20220310592Теплякова Т.В., Пьянков О.В., Скарнович М.О., Бормотов Н.И., Потешкина А.Л., Овчинникова А.С., Косогова Т.А., Магеррамова А.В., Маркович Н.А., Филиппова Е.И. Ингибитор репликации коронавируса SARS-CoV-2 на основе водного экстракта гриба Inonotus obliquus // Пат. РФ N 2741714С1; опубл. 28.01.2021 в Бюл. N 4.Teplyakova T.V., Pyankov O.V., Skarnovich M.O., Bormotov N.I., Poteshkina A.L., Ovchinnikova A.S., Kosogova T.A., Magerramova A.V., Markovich N.A., Filippova E.I. Ingibitor replikatsii koronavirusa SARS-CoV-2 na osnove vodnogo ekstrakta griba Inonotus obliquus [An inhibitor of SARS-CoV-2 coronavirus replication based on an aqueous extract of the fungus Inonotus obliquus]. Patent of the Russian Federation no. 2741714C1 published in Bulletin of Inventions no. 4. 28.01.2021. (In Russian)Казачинская Е.И., Чепурнов А.А., Кононова Ю.В., Шелемба А.А., Романюк В.В., Магомедов М.Г., Шестопалов А.М. Ингибирующая активность чайных композиций и их составляющих ингредиентов на репликацию SARS-COV-2 in vitro // Юг России: экология, развитие. 2022. Т. 17. N 2. С. 7690. DOI: 10.18470/1992-1098-2022-2-76-90Kazachinskaia E.I., Chepurnov А.А., Kononova Yu.V., Shelemba А.А., Romanyuk V.V., Magomedov M.G., Shestopalov А.М. Inhibitory activity of tea compositions and their constituent ingredients on SARS-COV-2 replication in vitro. South of Russia: ecology, development, 2022, vol. 17, no. 2, pp. 76-90. DOI: 10.18470/1992-1098-2022-2-76-90 (In Russian)Tolah A.M., Altayeb L.M., Alandijany T.A., Dwivedi V.D., El-Kafrawy S.A., Azhar E.I.' Computational and In Vitro Experimental Investigations Reveal Anti-Viral Activity of Licorice and Glycyrrhizin against Severe Acute Respiratory Syndrome Coronavirus 2 // Pharmaceuticals (Basel). 2021. V. 14. N 12. Article ID: 1216. DOI: 10.3390/ph14121216Tolah A.M., Altayeb L.M., Alandijany T.A., Dwivedi V.D., El-Kafrawy S.A., Azhar E.L Computational and In Vitro Experimental Investigations Reveal Anti-Viral Activity of Licorice and Glycyrrhizin against Severe Acute Respiratory Syndrome Coronavirus 2. Pharmaceuticals (Basel), 2021, vol. 14, no. 12, article id: 1216. DOI: 10.3390/ph14121216Чепурнов А.А., Шаршов К.А., Казачинская Е.И., Кононова Ю.В., Казачкова Е.А., и др. Антигенные свойства изолята коронавируса SARS-CoV-2/human/RUS/Nsk-FRCFTM-1/2020, выделенного от пациента в Новосибирске // Журнал инфектологии. 2020. Т. 12. N 3. C. 42-50. DOI: 10.22625/2072-6732-2020-12-3-42-50Chepurnov A.A., Sharshov K.A., Kazachinskaya E.I., Kononova Yu.V., Kazachkova E.A., Khripko O.P., Yurchenko K.S., Alekseev A.Yu., Voevoda M.I., Shestopalov A.M. Antigenic properties of sARs-CoV-2/human/RUs/nsk-FRCFtM-1/2020 coronavirus isolate from a patient in novosibirsk. Journal Infectology, 2020, vol. 12, no. 3, pp. 42-50. (In Russian) DOI: 10.22625/2072-6732-2020-12-3-42-50Kazachinskaia E.I., Chepurnov A.A., Shcherbakov D.N, Kononova Yu.V., Shanshin D.V., Romanova V.D., Khripko O.P., Saroyan T.A., Gulyaeva M.A., Voevoda M.I., Shestopalov A.M. IgG Study of Blood Sera of Patients with COVID-19 // Patogens. 2021. V. 10. N 11. P. 1421. DOI:10.3390/patogens10111421Kazachinskaia E.I., Chepurnov A.A., Shcherbakov D.N, Kononova Yu.V., Shanshin D.V., Romanova V.D., Khripko O.P., Saroyan T.A., Gulyaeva M.A., Voevoda M.I., Shestopalov A.M. IgG Study of Blood Sera of Patients with COVID-19. Patogens, 2021, vol. 10, no. 11, pp. 1421. DOI: 10.3390/patogens10111421Case J.B., Bailey A.L., Kim A.S., Chen R.E., Diamond M.S. Growth, detection, quantification, and inactivation of SARS-CoV-2 // Virology. 2020. N 548. P. 39-48. DOI: 10.1016/j.virol.2020.05.015Case J.B., Bailey A.L., Kim A.S., Chen R.E., Diamond M.S. Growth, detection, quantification, and inactivation of SARS-CoV-2. Virology, 2020, no. 548, pp. 39-48. DOI: 10.1016/j.virol.2020.05.015Hassan S.T.S., Berchova-Bimova K., Sudomova M., Malanik M., Smejkal K., Rengasamy K.R.R. In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice // J. Clin. Med. 2018. V. 7. Article ID: 283. DOI: 10.3390/jcm7090283Hassan S.T.S., Berchova-Bimova K., Sudomova M., Malanik M., Smejkal K., Rengasamy K.R.R. In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice. J. Clin. Med., 2018, vol. 7, article id: 283. DOI: 10.3390/jcm7090283Фисенко В.П. Руководство по экспериментальному (доклиническому) изучению новых фармакологических веществ / Под ред. Минздрав РФ, ЗАО «ИИА «Ремедиум, Москва. 2000. 398 с.Fisenko V.P. Guidelines for experimental (preclinical) study of new pharmacological substances. In accordance with the order. Ministry of Health of the Russian Federation, Closed Joint Stock Company «Remedium», Moscow, 2000, 398 p. (In Russian)Зибарева Л.Н., Еремина В.И., Иванова Н.А. Новые экдистероидоносные виды рода Silene L. и динамика содержания в них экдистерона // Раст. ресурсы. 1997. Т. 33. Bbim 3. С. 73-76.Zibareva L.N., Yeriomina V.I., Ivanova N.A. New ecdysteroid species of the genus Silene L. and the dynamics of the content of ecdysterone in them. Rast. Resources, 1997, vol. 33, no. 3, pp. 7376. (In Russian)Mamadalieva N., Zibareva L., Evrard-Todeschi N., Girault J.-P., Maria A., Ramazonov N.Sh., Saatov Z., Lafont R. New minor ecdysteroids from Silene viridiflora // Collect. Czech. Chem. Commun. 2004. V. 69. P. 1675-1680.Mamadalieva N., Zibareva L., Evrard-Todeschi N. Girault J.-P., Maria A., Ramazonov N.Sh., Saatov Z., Lafont R. New minor ecdysteroids from Silene viridiflora. Collect. Czech. Chem. Commun., 2004, vol. 69, pp. 1675-1680.Zibareva L.N., Seliverstova A.A., Suksamrarn A., Morozov S.V., Chernjak E.I. Phytoecdysteroids from the Aerial Part of Silene colpophylla // Chemistry of Natural Compounds. 2014. V. 50. N 3. P. 571-572. DOI: 10.1007/s10600-014-1021-xZibareva L.N., Seliverstova A.A., Suksamrarn A., Morozov S.V., Chernjak E.I. Phytoecdysteroids from the Aerial Part of Silene colpophylla. Chemistry of Natural Compounds, 2014, vol. 50, no. 3, pp. 571-572. DOI: 10.1007/s10600-014-1021-xФилоненко Е.С., Зибарева Л.Н. Экдистероиды и флавоноиды Silene graefferi // Химия растительного сырья. 2021. N1. С. 175-182. DOI: 10.14258/jcprm.2021018294Filonenko E.S., Zubareva L.N. Ecdysteroids and flavonoids Silene graefferi. Chemistry of plant raw materials, 2021, no. 1, pp. 175-182. (In Russian) DOI: 10.14258/jcprm.2021018294Zibareva L. Distribution and levels of phytoecdysteroids in plants of genus Silene during development // Archives of insect biochemistry and physiology. 2000. V. 43. P. 1-8.Zibareva L. Distribution and levels of phytoecdysteroids in plants of genus Silene during development. Archives of insect biochemistry and physiology, 2000, vol. 43, pp. 1-8.Зибарева Л.Н., Амосова Е.Н., Крылова С.Г., Зуева Е.П., Рыбалкина О.Ю., Плотников М.Б., Алиев О.И., Васильев А.С., Анищенко А.М., Суслов Н.И., Нестерова Ю.В., Поветьева Т.Н., Афанасьева О.Г., Эрст А.А., Разина Т.Г., Сафонова Е.А., Киселева Е.А. Растения родов Silene L. и Lychnis L. (Caryophyllaceae): состав химических компонентов и биологическая активность. Томск: Издательство Томского государственного университета, 2021. 496 с.Zibareva L.N., Amosova E.N., Krylova S.G., Zueva E.P., Rybalkina O.Y., Plotnikov M.B., Aliyev O.I., Vasiliev A.S., Anishchenko A.M., Suslov N.I., Nesterova Yu.V., Povetyeva T.N., Afanasyeva O.G., Erst A.A., Razina T.G., Safonova E.A., Kiseleva E.A. Rasteniya rodov Silene L. i Lychnis L. (Caryophyllaceae): sostav khimicheskikh komponentov i biologicheskaya aktivnost' [Plants of the genera Silene L. and Lychnis L. (Caryophyllaceae): composition of chemical components and biological activity]. Tomsk, Tomsk State University Publ., 2021, 496 p. (In Russian)Mamadalieva N., Zibareva L., Saatov Z. Phytoecdysteroids of Silene linicola // Chemistry of Natural Compounds. 2002. V. 38. P. 268-271.Mamadalieva N., Zibareva L., Saatov Z. Phytoecdysteroids of Silene linicola. Chemistry of Natural Compounds, 2002, vol. 38, pp. 268-271.Зибарева Л.Н., Балтаев У.А., Ревина Т.А., Абубакиров Н.К. Фитоэкдистероиды растений рода Лихнис // Химия природ. соедин. 1991. N 4. C. 584-585.Zibareva L.N., Baltaev U.A., Revina T.A., Abubakirov N.K. Phytoecdysteroids of plants of the genus Lychnis. Chemistry of natural compounds. 1991, no. 4, pp. 584-585. (In Russian)Зибарева Л.Н., Саатов З., Абубакиров Н.К. Стахистерон D, витикостерон Е и а-экдизон из Lychnis chalcedonica // Химия природ. соедин. 1991. N 4. C. 585-586.Zibareva L.N., Saatov Z., Abubakirov N.K. Stachisterone D, viticosterone E and a-ecdysone from Lychnis chalcedonica. Chemistry of natural compounds. 1991, no. 4, pp. 585-586. (In Russian)Wang H., Zhang Y., Huang B., Huang B., Deng W., Quan Y., Wang W., Xu W., Zhao Y., Li N., Zhang J. et al. Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2 // Cell. 2020. V. 182. N 3. 713-721.e9. DOI: 10.1016/j.cell.2020.06.008Wang H., Zhang Y., Huang B., Huang B., Deng W., Quan Y., Wang W., Xu W., Zhao Y., Li N., Zhang J. et al. Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2. Cell, 2020, vol. 182, no. 3, pp. 713-721.e9. DOI: 10.1016/j.cell.2020.06.008Popovici V., Bucur L., Gird C.E., Rambu D., Calcan S.I., Cucolea E.I., Costache T., Ungureanu-Iuga M., Oroian M., Mironeasa S., Schroder V., Ozon E.-A., Lupuliasa D., Caraiane A., Badea V. Antioxidant, Cytotoxic, and Rheological Properties of Canola Oil Extract of Usnea barbata (L.) Weber ex F.H. Wigg from Calimani Mountains, Romania // Plants (Basel). 2022. V. 11. N 7:854. DOI: 10.3390/plants11070854Popovici V., Bucur L., Gird C.E., Rambu D., Calcan S.I., Cucolea E.I., Costache T., Ungureanu-Iuga M., Oroian M., Mironeasa S., Schroder V., Ozon E.-A., Lupuliasa D., Caraiane A., Badea V. Antioxidant, Cytotoxic, and Rheological Properties of Canola Oil Extract of Usnea barbata (L.) Weber ex F.H. Wigg from Calimani Mountains, Romania. Plants (Basel), 2022, vol. 11, no. 7, article id: 854. DOI: 10.3390/plants11070854Trujillo-Correa A.I., Quintero-Gil D.C., Diaz-Castillo F., Quinones W., Robledo S. M., Martinez-Gutierrez M. In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting // BMC Complement Altern Med. 2019. V. 19. N 1. P. 298. DOI: 10.1186/s12906-019-2695-1Trujillo-Correa A.I., Quintero-Gil D.C., Diaz-Castillo F., Quinones W., Robledo S. M., Martinez-Gutierrez M. In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting. BMC Complement Altern Med, 2019, vol. 19, no. 1, pp. 298. DOI: 10.1186/s12906-019-2695-1Galvao J., Davis B., Tilley M., Normando E., Duchen M.R., Cordeiro M.F. Unexpected low-dose toxicity of the universal solvent DMSO // FASEB J. 2014. V. 28. N 3. P. 1317-1330. DOI: 10.1096/fj.13-235440Galvao J., Davis B., Tilley M., Normando E., Duchen M.R., Cordeiro M.F. Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J., 2014, vol. 28, no. 3, pp. 1317-1330. DOI: 10.1096/fj.13-235440Gironi B., Oliva R., Petraccone L., Paolantoni M., Morresi A. , Vecchio P.D., Sassi P. Solvation properties of raft-like model membranes // Biochim Biophys Acta Biomembr. 2019. V. 1861. N 11:183052. DOI: 10.1016/j.bbamem.2019.183052Gironi B., Oliva R., Petraccone L., Paolantoni M., Morresi A. , Vecchio P.D., Sassi P. Solvation properties of raft-like model membranes. Biochim Biophys Acta Biomembr, 2019, vol. 1861, no. 11, article id: 183052. DOI: 10.1016/j.bbamem.2019.183052Kanjanasirirat P., Suksatu A., Manopwisedjaroen S., Munyoo B. , Tuchinda P-, Jearawuttanakul K., Seemakhan S., Charoensutthivarakul S., Wongtrakoongate P., Rangkasenee N. et al. High-content screening of Thai medicinal plants reveals Boesenbergia rotunda extract and its component Panduratin A as anti-SARS-CoV-2 agents // Sci Rep. 2020. V. 10. N 1:19963. DOI: 10.1038/s41598-020-77003-3Kanjanasirirat P., Suksatu A., Manopwisedjaroen S., Munyoo B. , Tuchinda P-, Jearawuttanakul K., Seemakhan S., Charoensutthivarakul S., Wongtrakoongate P., Rangkasenee N. et al. High-content screening of Thai medicinal plants reveals Boesenbergia rotunda extract and its component Panduratin A as anti-SARS-CoV-2 agents. Sci Rep., 2020, vol. 10, no. 1, article id: 19963. DOI: 10.1038/s41598-020-77003-3Xie P., Fang Y., Shen Z., Shao Y., Ma Q., Yang Z., Zhao J., Li H., Li R., Dong S., Wen W., Xia X. Broad antiviral and anti-inflammatory activity of Qingwenjiere mixture against SARS-CoV-2 and other human coronavirus infections // Phytomedicine. 2021. N 93:153808. DOI: 10.1016/j.phymed.2021.153808Xie P., Fang Y., Shen Z., Shao Y., Ma Q., Yang Z., Zhao J., Li H., Li R., Dong S., Wen W., Xia X. Broad antiviral and anti-inflammatory activity of Qingwenjiere mixture against SARS-CoV-2 and other human coronavirus infections. Phytomedicine, 2021, no. 93, article id: 153808. DOI: 10.1016/j.phymed.2021.153808Zannella C., Giugliano R., Chianese A., Buonocore C., Vitale G.A., Sanna G., Sarno F., Manzin A., Nebbioso A., Termolino P., Altucci L., Massimiliano G., de Pascale D., Franci G. Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1 // Viruses. 2021. V. 13. N 7. P. 1263. DOI: 10.3390/v13071263Zannella C., Giugliano R., Chianese A., Buonocore C., Vitale G.A., Sanna G., Sarno F., Manzin A., Nebbioso A., Termolino P., Altucci L., Massimiliano G., de Pascale D., Franci G. Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1. Viruses, 2021, vol. 13, no. 7, pp. 1263. DOI: 10.3390/v13071263Nie C., Trimpert J., Moon S., Haag R., Gilmore K., Kaufer B.B., Seeberger P.H. In vitro efficacy of Artemisia extracts against SARS-CoV-2 // Virol J. 2021. V. 18. N 1. P. 182. DOI: 10.1186/s12985-021-01651-8Nie C., Trimpert J., Moon S., Haag R., Gilmore K., Kaufer B.B., Seeberger P.H. In vitro efficacy of Artemisia extracts against SARS-CoV-2. Virol J., 2021, vol. 18, no. 1, pp. 182. DOI: 10.1186/s12985-021-01651-8Chuang S.-T., Buchwald P. Broad-Spectrum Small-Molecule Inhibitors of the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction from a Chemical Space of Privileged Protein Binders // Pharmaceuticals (Basel). 2022. V. 15. N 9. P. 1084. DOI: 10.3390/ph15091084Chuang S.-T., Buchwald P. Broad-Spectrum Small-Molecule Inhibitors of the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction from a Chemical Space of Privileged Protein Binders. Pharmaceuticals (Basel), 2022, vol. 15, no. 9, pp. 1084. DOI: 10.3390/ph15091084Artese A., Svicher V., Costa G., Salpini R., Maio V.C.D., Alkhatib M., Ambrosio F.A., Santoro M.M., Assaraf Y.G., Alcaro S., Ceccherini-Silberstein F. Current status of antivirals and druggable targets of SARS CoV-2 and other human pathogenic coronaviruses // Drug Resist Updat. 2020. N 53:100721. DOI: 10.1016/j.drup.2020.100721Artese A., Svicher V., Costa G., Salpini R., Maio V.C.D., Alkhatib M., Ambrosio F.A., Santoro M.M., Assaraf Y.G., Alcaro S., Ceccherini-Silberstein F. Current status of antivirals and druggable targets of SARS CoV-2 and other human pathogenic coronaviruses. Drug Resist Updat., 2020, no. 53, article id: 100721. DOI: 10.1016/j.drup.2020.100721Liu X.-H., Cheng T., Liu B.-Y., Chi J., Shu T., Wang T. Structures of the SARS-CoV-2 spike glycoprotein and applications for novel drug development // Front Pharmacol. 2022. N 13:955648. DOI: 10.3389/fphar.2022.955648Liu X.-H., Cheng T., Liu B.-Y., Chi J., Shu T., Wang T. Structures of the SARS-CoV-2 spike glycoprotein and applications for novel drug development. Front Pharmacol., 2022, no. 13, article id: 955648. DOI: 10.3389/fphar.2022.955648Cao J., Liu Y., Zhou M., Dong S., Hou Y., Jia X., Lan X., Zhang Y., Guo J., Xiao G., Wang W. Screening of Botanical Drugs against SARS-CoV-2 Entry Reveals Novel Therapeutic Agents to Treat COVID-19 // Viruses. 2022. V. 14. N 2. P. 353. DOI: 10.3390/v14020353Cao J., Liu Y., Zhou M., Dong S., Hou Y., Jia X., Lan X., Zhang Y., Guo J., Xiao G., Wang W. Screening of Botanical Drugs against SARS-CoV-2 Entry Reveals Novel Therapeutic Agents to Treat COVID-19. Viruses, 2022, vol. 14, no. 2, pp. 353. DOI: 10.3390/v14020353Kicker E., Tittel G., Schaller T., Pferschy-Wenzig E.-M., Zatloukal K., Bauer R. SARS-CoV-2 neutralizing activity of polyphenols in a special green tea extract preparation // Phytomedicine. 2022. N 98:153970. DOI: 10.1016/j.phymed.2022.153970Kicker E., Tittel G., Schaller T., Pferschy-Wenzig E.-M., Zatloukal K., Bauer R. SARS-CoV-2 neutralizing activity of polyphenols in a special green tea extract preparation. Phytomedicine, 2022, no. 98, article id: 153970. DOI: 10.1016/j.phymed.2022.153970Sahoo M.R., Umashankar M.S., Varier R.R. The research updated and prospects of herbal hard-boiled lozenges: a classical platform with promising drug delivery potential // Int J App Pharm. 2021. V. 13. N 2. P. 1-13. URL: https://innovareacademics.in/journals/index.php/ijap/article/view/40165 (дата обращения: 30.11.2022)Sahoo M.R., Umashankar M.S., Varier R.R. The research updated and prospects of herbal hard-boiled lozenges: a classical platform with promising drug delivery potential. Int J App Pharm., 2021, vol. 13, no. 2, pp. 1-13. Available at: https://innovareacademics.in/journals/index.php/ijap/article/view/40165 (accessed 30.11.2022)

The authors declare that there are no conflicts of interest present.