ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2022-4-111-129ecodag-2666Research ArticleЭКОЛОГИЯ МИКРООРГАНИЗМОВИнгибирующая активность сухих этанольных экстрактов Artemisia spp. на репликацию SARS‐CoV‐2 in vitroInhibitory activity of dry ethanol extracts of Artemisia spp. on SARS‐CoV‐2 replication in vitrohttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.КаzachinskaiaЕ. I.

Елена И. Казачинская, доктор биологических наук, ведущий научный сотрудник

630559 Новосибирская область, г. Новосибирск, рабочий поселок Кольцово, 32‐1

Тел. +79095307441

Еlena I. Каzachinskaia, Doctor of Biology, Leading Scientist

32‐1, working settlement of Koltsovo, Novosibirsk, Novosibirskaya oblast 630559

Tel. +79095307441

lena.kazachinskaia@mail.ruhttps://orcid.org/0000-0002-0485-4818РомановаВ. Д.RomanovaV. D.

Валерия Д. Романова 

Новосибирск

Valeriya D. Romanova

Novosibirsk

https://orcid.org/0000-0001-9102-6756ИвановаА. В.IvanоvaA. V.

Алла В. Иванова 

Новосибирск

Alla V. Ivanоva

Novosibirsk

https://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovА. А.

Александр А. Чепурнов 

Новосибирск

Аlexander А. Chepurnov

Novosibirsk

https://orcid.org/0000-0001-9131-677XМуртазалиеваЗ. А.MurtazalievaZ. A.

Зумруд А. Муртазалиева 

Махачкала

Zumrud A. Murtazalieva

Makhachkala

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.КоnonovaYu. V.

Юлия В. Кононова 

Новосибирск

Yulia V. Коnonova

Novosibirsk

https://orcid.org/0000-0002-1835-8532ШаулоД. Н.ShauloD. N.

Дмитрий Н. Шауло 

Новосибирск

Dmitri N. Shaulo

Novosibirsk

https://orcid.org/0000-0002-6860-9168РоманюкВ. В.RomanyukV. V.

Владимир В. Романюк 

Новосибирск

Vladimir V. Romanyuk

Novosibirsk

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovА. М.

Александр М. Шестопалов 

Новосибирск

Аlexander М. Shestopalov

Novosibirsk

Научно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» Cибирского отделения Российской Академии наук (ФИЦ ФТМ СО РАН); ФБУН Государственный научный центр вирусологии и биотехнологии «Вектор» (ФБУН ГНЦ ВБ «Вектор»)Research Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; “Vector” State Research Centre of Virology and Biotechnology, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» Cибирского отделения Российской Академии наук (ФИЦ ФТМ СО РАН)Research Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of SciencesФБУН Государственный научный центр вирусологии и биотехнологии «Вектор» (ФБУН ГНЦ ВБ «Вектор»)“Vector” State Research Centre of Virology and Biotechnology, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareДагестанский государственный медицинский университетDagestan State Medical UniversityЦентральный сибирский ботанический сад (ЦСБС) СО РАНCentral Siberian Botanical Garden, Siberian Branch, Russian Academy of SciencesООО Научно‐производственная фирма (НПФ) «Золотая долина»Zolotaya Dolina Research and Production Co.202229122022174111‐129Copyright © Казачинская Е.И., Романова В.Д., Иванова А.В., Чепурнов А.А., Муртазалиева З.А., Кононова Ю.В., Шауло Д.Н., Романюк В.В., Шестопалов А.М., 20222022Казачинская Е.И., Романова В.Д., Иванова А.В., Чепурнов А.А., Муртазалиева З.А., Кононова Ю.В., Шауло Д.Н., Романюк В.В., Шестопалов А.М.Каzachinskaia Е.I., Romanova V.D., Ivanоva A.V., Chepurnov А.А., Murtazalieva Z.A., Коnonova Y.V., Shaulo D.N., Romanyuk V.V., Shestopalov А.М.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2666Цель

Цель. Анализ in vitro ингибирующей активности сухих этанольных экстрактов некоторых Artemisia spp., произрастающих в Новосибирской области, на репликацию SARS‐CoV‐2.

Материалы и методы

Материалы и методы. Лабораторный штамм SARS‐CoV-2/human/RUS/Nsk‐FRCFTM‐1/2020 пассирован на культуре клеток Vero. Приготовлены сухие этанольные экстракты частей растения (стеблей, цветов, листьев) шести видов полыни: Artemisia vulgaris L., A. glauca (Pall. Ex Willd.), A. dracunculus L. (из трех мест произрастания), A. absinthium L., A. frigida Willd., A. sieversiana Ehrh. ex Willd. Сухие экстракты растворяли в ДМСО. Анализ in vitro ингибирующей активности экстрактов на репликацию SARS‐CoV‐2 (в инфекционном титре 103 TЦПД50/мл) проводили в культуре клеток Vero E6 методом прямой инактивации (нейтрализации) вирионов, а также по схемам «профилактики» и «лечения» клеток. Образцы сравнения – сухие этанольные экстракты чаги, специи гвоздики и черного чая.

Результаты

Результаты. Экстракты листьев Artemisia spp. оказались наиболее эффективны при прямой инактивации вирионов по равной и убывающей активности – A. vulgaris, A. dracunculus*, A. absinthium, A. dracunculus***, A. dracunculus**, A. frigidа, A. glauca и A. sieversiana в диапазоне 50%‐ных эффективных концентраций 1,10±0,24 – 11,72±2,89 мкг/мл. Экстракты цветов A. vulgaris, A. glauca, A. dracunculus*, A. dracunculus**, A. dracunculus***, A. frigida и A. sieversiana, также содержат БАВ, действующие как деструктивно на вирионы, так и после проникновения вируса в клетки. Для экстрактов стеблей стабильно высокие значения EC50 выявлены для A. glauca (6,84±1,35; 7,81±2,00; 14,06±3,06 мкг/мл) по результатам трех схем экспериментов.

Заключение

Заключение. Полученные результаты могут стать основой для разработки недорогих отечественных препаратов для лечения и/или профилактики COVID‐19.

Aim

Aim. In vitro analysis of the inhibitory activity of dry ethanol extracts of some Artemisia spp. growing in the Novosibirsk region for SARS‐CoV‐2 replication.

Materials and Methods

Materials and Methods. The laboratory strain SARS‐CoV‐2/human/RUS/Nsk‐FRCFTM‐1/2020 was passed on Vero cell culture. Dry ethanol extracts of plant parts (stems, flowers, leaves) of six types of Artemisia were prepared. The types used were: A. vulgaris L.; A. glauca (Pall. Ex Willd.); A. dracunculus L. (from three growth locations); A. absinthium L.; A. frigida Willd.; and A. sieversiana Ehrh. ex Willd. Dry extracts were dissolved in DMSO. In vitro analysis of the inhibitory activity of extracts against SARS‐CoV‐2 (an infectious titer of 103 TCID50/ml) replication was performed in a Vero E6 cell culture. To do this, the method of direct inactivation (neutralization) of virions, as well as schemes of “preventive” and “therapeutic” of cells, were used. Comparison samples were dry ethanol extracts of Inonotus obliquus, Syzygium aromaticum L. and Camellia sinensis L.

Results

Results. Extracts of leaves of Artemisia spp. proved to be most effective in direct inactivation of virions. By equal and decreasing activity these are the species: A. vulgaris; A. dracunculus*; A. absinthium; A. dracunculus***; A. dracunculus**; A. frigidа; A. glauca; and A. sieversiana with a 50% effective concentration of range 1.10±0.24 – 11.72±2.89 μg/ml. Extracts of flowers of A. vulgaris, A.glauca, A. dracunculus*, A. dracunculus**, A. dracunculus***, A. frigida and A. sieversiana also contain biologically active substances which act both destructively on virions and after the virus has entered cells. For extracts of stems consistently high values of EC50 were found for A. glauca (6.84±1.35; 7.81±2.00 and 14.06±3.06 μg/ml) according to the results of three experimental schemes.

Conclusion

Conclusion. The results obtained can become the basis for the development of inexpensive domestic drugs for the treatment and/or prevention of COVID‐19.

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The authors declare that there are no conflicts of interest present.