ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2022-4-61-78ecodag-2662Research ArticleЭКОЛОГИЯ ГРИБОВECOLOGY OF FUNGIИспользование экологически безопасного микромицета рода Rhodotorula для получения кормового каротинсодержащего концентратаUse of environmentally safe micromycetes of the genus Rhodotorula to obtain fodder carotene‐containing concentratehttps://orcid.org/0000-0002-7288-8569КолпаковаВ. В.KolpakovaV. V.

Валентина В. Колпакова, доктор технических наук, профессор, главный научный сотрудник

140051 Московская область, г. Красково, ул. Некрасова 11

Тел. +79152858450

Valentina V. Kolpakova, Doctor оf Technical Sciences, Professor, Chief Researcher

11 Nekrasova, St, Kraskovo, Moscow Region 140051

Tel. +79152858450

val-kolpakova@rambler.ruУлановаР. В.UlanovaR. V.

Рузалия В. Уланова  

Красково; Москва

Ruzaliya V. Ulanova

Kraskovo; Moscow

https://orcid.org/0000-0003-2171-0522КуликовД. С.KulikovD. S.

Денис С. Куликов  

Красково

Denis S. Kulikov

Kraskovo

https://orcid.org/0000-0001-8393-9256ГулаковаВ. А.GulakovaV. A.

Валентина А. Гулакова  

Красково

Valentina A. Gulakova

Kraskovo

https://orcid.org/0000-0002-9811-9602ВасильеваЛ. В.VasilyevaL. V.

Лина В. Васильева  

Москва

Lina V. Vasilyeva

Moscow

БерестовскаяЮ. Ю.BerestovskayaYu. Yu.

Юлия Ю. Берестовская  

Москва

Yulia Yu. Berestovskaya

Moscow

https://orcid.org/0000-0001-5166-4462ЧеремныхЕ. Г.CheremnykhE. G.

Елена Г. Черемных  

Москва

Elena G. Cheremnykh

Moscow

https://orcid.org/0000-0001-6978-8017АшихминА. А.AshikhminA. A.

Александр А. Ашихмин  

Пущино

Alexander A. Ashikhmin 

Pushchino

Всероссийский научно‐исследовательский институт крахмала и переработки крахмалсодержащего сырья – филиал ФГБНУ «Федеральный исследовательский центр картофеля имени А.Г. Лорха»РоссияAll‐Russian Research Institute of Starch and Processing of Starch‐Containing Raw Materials – Branch of A.G. Lorkha Federal Potato Research CentreRussian FederationВсероссийский научно‐исследовательский институт крахмала и переработки крахмалсодержащего сырья – филиал ФГБНУ «Федеральный исследовательский центр картофеля имени А.Г. Лорха»; Институт микробиологии имени С.Н. Виноградского Федерального исследовательского центра «Фундаментальные основы биотехнологии» Российской академии наукРоссияAll‐Russian Research Institute of Starch and Processing of Starch‐Containing Raw Materials – Branch of A.G. Lorkha Federal Potato Research Centre; S.N. Vinogradsky Institute of Microbiology, Fundamental Foundations of Biotechnology Federal Research Centre, Russian Academy of SciencesRussian FederationИнститут микробиологии имени С.Н. Виноградского Федерального исследовательского центра «Фундаментальные основы биотехнологии» Российской академии наукРоссияS.N. Vinogradsky Institute of Microbiology, Fundamental Foundations of Biotechnology Federal Research Centre, Russian Academy of SciencesRussian FederationНаучный центр психического здоровьяРоссияMental Health Research CenterRussian FederationИнститут фундаментальных проблем биологии РАН, Федеральный исследовательский центр «Пущинский научный центр биологических исследований РАН»РоссияInstitute of Physicochemical and Biological Problems in Soil Science, Pushchino Scientific Centre for Biological Research, Russian Academy of SciencesRussian Federation20222912202217461‐78Copyright © Колпакова В.В., Уланова Р.В., Куликов Д.С., Гулакова В.А., Васильева Л.В., Берестовская Ю.Ю., Черемных Е.Г., Ашихмин А.А., 20222022Колпакова В.В., Уланова Р.В., Куликов Д.С., Гулакова В.А., Васильева Л.В., Берестовская Ю.Ю., Черемных Е.Г., Ашихмин А.А.Kolpakova V.V., Ulanova R.V., Kulikov D.S., Gulakova V.A., Vasilyeva L.V., Berestovskaya Y.Y., Cheremnykh E.G., Ashikhmin A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2662Цель работы

Цель работы. Изучение возможности использования экологически безопасного штамма дрожжей рода Rhodotorula для биоконверсии вторичного продукта переработки гороховой муки на белковый концентрат (сыворотки) в кормовую каротиноидсодержащую биомассу.

Материал и методы

Материал и методы. Использовали новый штамм Rhodotorula mucilaginosa 111 и вторичные продукты переработки гороховой и нутовой муки на белковые концентраты и картофеля на крахмал (сыворотку). Применяли стандартные и специальные методы анализа сыворотки и микробно‐растительного концентрата (КМРК): химические, биохимические, микробиологические, определение токсичности с инфузориями.

Результаты

Результаты. Определены оптимальные режимы выращивания R. mucilaginosa 111 на гороховой сыворотке (температура 16,9оС; pH 7,8; количество посевного материала 1,85%). Биомассы на гороховой сыворотке синтезировалось больше, чем на нутовой и картофельной сыворотке – 81 г/дм3. Массовая доля белка в биомассе – 58,90±3,03% на сухое вещество, скор незаменимых аминокислот – 119–243%. Липиды включали 20% насыщенных и 78% ненасыщенных жирных кислот, линолевой кислоты – 45,26±0,70%, олеиновой – 24,04±0,76%, пальмитолеиновой – 6,46±0,31%, пальмитиновой – 13,70±0,81%. Дрожжи продуцировали производные фитоина, торулен, β‐каротин, торулародин, фитоин. КМРК из гороховой сыворотки стимулировал рост инфузории Tetrahymena pyriformis на 29,1%, из нутовой сыворотки – на 18,6% интенсивнее, чем дистиллированная вода, картофельная сыворотка понижала коэффициент ее роста.

Заключение

Заключение. Сухая биомасса экологически безопасного нового штамма дрожжей R. mucilaginosa 111 содержала необходимые для кормления животных полноценные белки, липиды, минералы, каротиноиды; жидкую гороховую сыворотку возможно использовать для ее биоконверсии и в целях исключения загрязнения окружающей среды.

Aim

Aim. The aim of the work was to study the possibility of using an environmentally friendly strain of yeast of the genus Rhodotorula for the bioconversion into fodder carotenoid‐containing biomass of the secondary product of processing pea flour into a protein concentrate (whey).

Material and Methods

Material and Methods. We used a new strain of Rhodotorula mucilaginosa 111 and by‐products of processing pea and chickpea flour into protein concentrates and potatoes into starch (whey). We used standard and special methods for the analysis of serum and microbial‐vegetable concentrate (FMVC) namely: chemical; biochemical; microbiological; and the determination of toxicity with ciliates.

Results

Results. Optimal conditions for growing R. mucilaginosa 111 on pea whey were determined (temperature 16.9°C, pH 7.8, amount of inoculum 1.85%). More biomass was synthesized on pea whey than on chickpea and potato whey – 81 g/dm3. The mass fraction of protein in the biomass is 58.90±3.03% on dry matter and the rate of essential amino acids is 119– 243%. Lipids included 20% saturated and 78% unsaturated fatty acids, linoleic acid – 45.26±0.70%, oleic – 24.04±0.76%, palmitoleic – 6.46±0.31%, palmitic – 13.70±0.81%. The yeast produced phytoin derivatives, torulene, β‐carotene, torularodin and phytoin. FMVC from pea whey stimulated the growth of ciliates Tetrahymena pyriformis by 29.1%, from chickpea whey (by 18.6% more intensively than distilled water), while potato whey reduced its growth rate.

Conclusion

Conclusion. The dry biomass of the ecologically safe new yeast strain R. mucilaginosa 111 contained complete proteins, lipids, minerals, and carotenoids necessary for feeding animals. Thus liquid pea whey can be used for its biokonversions, while avoiding environmental pollution.

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The authors declare that there are no conflicts of interest present.