ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2022-3-54-62ecodag-2578Research ArticleЭКОЛОГИЯ ЖИВОТНЫХECOLOGY OF ANIMALSВлияние pН на эмбриональное и личиночное развитие малоазиатской лягушки, Rana macrocnemisThe effect of pH on embryonal and larval development of the Caucasian Brown Frog, Rana macrocnemishttps://orcid.org/0000-0003-0460-9895ГамидоваДж. М.GamidovaJ. M.

Гамидова Джамиля М. - аспирант кафедры зоологии и физиологии.

367000, Махачкала, ул. Дзержинского, 12.

Тел. +79896747149

Jamilya M. Gamidova - post‐graduate student, Department of Zoology and Physiology, Dagestan State University.

12 Dzerzhinsky St, Makhachkala, 367000.

Tel. +79896747149

djamka_90@mail.ruhttps://orcid.org/0000-0002-9041-6880РабадановаА. И.RabadanovaA. I.

Рабаданова Аминат И.

Махачкала.

Rabadanova Aminat I.

Makhachkala.

Дагестанский государственный университетDagestan State University2022091020221735462Copyright © Гамидова Д.М., Рабаданова А.И., 20222022Гамидова Д.М., Рабаданова А.И.Gamidova J.M., Rabadanova A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2578Цель

Цель. Изучить влияние различных значений рН на особенности эмбрионального и личиночного развития Rana macrocnemis.

Материал и методы

Материал и методы. В   работе   использовались   фрагменты   из 6–7 кладок R. macrocnemis, собранных на территории Предгорного Дагестана. Эксперимент включал две серии опытов: в 1‐й серии изучался весь цикл эмбрионально‐личиночного развития малоазиатской   лягушки,   а   во   2‐й   –   только   постэмбриональное развитие R. macrocnemis. Икринки поштучно размещались в контейнеры с различными значениями рН (4.0, 5.0 и 9.0).

Результаты

Результаты. Наиболее      уязвимыми      периодами      в      развитии R. macrocnemis являются стадии нейрулы, формирования конечностей и метаморфоза. При рН 4.0 снижается потребление кислорода личинками, что сопровождается угнетением пищевого поведения и снижением интенсивности обменных процессов. Кроме того, рН 4.0 оказывает   негативное   влияние   на   выживаемость   эмбрионов   и личинок. При рН 5.0 отмечены изменения, несущие в основном адаптивный характер. На фоне ускоренного развития эмбрионов при этих значениях рН отмечается значительное снижение длины и массы их тела. С переходом к личиночному развитию наблюдается увеличение размеров тела на фоне небольшого прироста массы. Отмечено также удлинение продолжительности периода от начала формирования конечностей до окончания метаморфоза.

Заключение

Заключение. Полученные данные могут быть использованы при мониторинге уровня кислотности среды и содержания кислорода в естественных водоемах в период нереста, что поможет внести ясность в вопрос о сокращении численности малоазиатской лягушки на территории Дагестана.

Aim

Aim. To study the effect of different pH values on the characteristics of the embryonic and larval development of Rana macrocnemis.

Material and methods

Material and methods. Fragments from 6–7 clutches of R. macrocnemis collected on the territory of Foothill Dagestan were used in the work. The experiment  included  two  series  of  experiments:  in  the  1st  series,  the entire cycle of embryonic‐larval development of the R. macrocnemis frog was  studied,  and  in  the  2nd,  only  the  postembryonic  development  of R. macrocnemis was studied. Eggs were individually placed in containers with different pH values (4.0, 5.0, and 9.0).

Results

Results.    The    most    vulnerable    periods    in    the    development    of R. macrocnemis are the stages of neurula, limb formation, and metamorphosis. At pH 4.0, oxygen consumption by larvae decreases, accompanied by suppression of feeding behavior and a decrease in the intensity of metabolic processes. In addition, pH 4.0 has a negative effect on the survival of embryos and larvae. At pH 5.0, changes are noted that are mainly of an adaptive nature. Against the background of the accelerated development of embryos at these pH values, a significant decrease  in  the  length  and  weight  of  their  body  is  noted.  With  the transition to larval development, an increase in body size is observed against the background of a small increase in weight. An elongation of the duration of the period from the beginning of the formation of limbs to the end of metamorphosis was also noted.

Conclusion

Conclusion. The data obtained can be used in monitoring the level of acidity of the environment and the oxygen content in natural water bodies during the spawning period, which will help clarify the issue of the reduction in the number of R. macrocnemis frogs in Dagestan.

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The authors declare that there are no conflicts of interest present.