ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2022-2-76-90ecodag-2488Research ArticleЭКОЛОГИЯ МИКРООРГАНИЗМОВИнгибирующая активность чайных композиций и их составляющих ингредиентов на репликацию SARS‐COV‐2 in vitroInhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitrohttps://orcid.org/0000-0002-1856-6147КазачинскаяЕ. И.КаzachinskaiaЕ. I.

Елена И. Казачинская, доктор биологических наук, ведущий научный сотрудник

630559 Новосибирская область, р/п Кольцово, 32‐1

Тел. +79095307441

Еlena I. Каzachinskaia, Doctor of Biology, Leading Researcher of the Research Institute of Virology

Prospekt Koltsovo 32‐1, Novosibirsk, Novosibirskiy region, 630559

Теl. +79095307441

lena.kazachinsksia@mail.ruhttps://orcid.org/0000-0002-5966-8633ЧепурновА. А.ChepurnovА. А.

Александр А. Чепурнов

Новосибирск

Аlexander А. Chepurnov

Novosibirsk

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.КоnonovaYu. V.

Юлия В. Кононова

Новосибирск

Yulia V. Коnonova

Novosibirsk

https://orcid.org/0000-0001-7840-1478ШелембаА. А.ShelembaА. А.

Арсения А. Шелемба

Новосибирск

Аrseniya А. Shelemba

Novosibirsk

https://orcid.org/0000-0002-6860-9168РоманюкВ. В.RomanyukV. V.

Владимир В. Романюк

Новосибирск

Vladimir V. Romanyuk

Novosibirsk

https://orcid.org/0000-0003-1897-6784МагомедовМ. Г.MagomedovM. G.

Магомед Г. Магомедов

Махачкала

Magomed G. Magomedov

Makhachkala

https://orcid.org/0000-0002-9734-0620ШестопаловА. М.ShestopalovА. М.

Александр М. Шестопалов

Новосибирск

Аlexander М. Shestopalov

Novosibirsk

Научно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукРоссияResearch Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine (FRCFTM), Siberian Branch, Russian Academy of SciencesRussian FederationООО Научно‐производственная фирма (НПФ) «Золотая долина»РоссияZolotaya Dolina Research and Production CompanyRussian FederationДагестанский государственный медицинский университетРоссияDagestan State Medical UniversityRussian Federation2022140720221727690Copyright © Казачинская Е.И., Чепурнов А.А., Кононова Ю.В., Шелемба А.А., Романюк В.В., Магомедов М.Г., Шестопалов А.М., 20222022Казачинская Е.И., Чепурнов А.А., Кононова Ю.В., Шелемба А.А., Романюк В.В., Магомедов М.Г., Шестопалов А.М.Каzachinskaia Е.I., Chepurnov А.А., Коnonova Y.V., Shelemba А.А., Romanyuk V.V., Magomedov M.G., Shestopalov А.М.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2488Цель

Цель. Исследование ингибирующей активности водных экстрактов чайных композиций и входящего в них растительного сырья на репликацию SARS‐CoV‐2 in vitro.

Материал и методы

Материал и методы. Лабораторный штамм SARS‐CoV‐ 2/human/RUS/Nsk‐FRCFTM‐1/2020 был пассирован на культуре клеток Vero. Для приготовления водных экстрактов использовали шесть видов экспериментальных чайных композиций (не содержащих ароматизаторов) на основе черного и зеленого чая или цветов гибискуса, а также индивидуальные составляющие ингредиенты. Противовирусную (ингибирующую) активность растительных экстрактов исследовали in vitro по классической схеме нейтрализации (инактивации) вируса.

Результаты

Результаты. При сравнении с основным контрольным образцом водного экстракта чаги с 50%‐ной эффективной концентрацией, равной 13,72±2,99 мкг/мл против 103 ТЦПД50/мл SARS‐CoV‐2, превосходящая активность обнаружена для экстрактов специи гвоздики и черного чая из Непала. Приблизительно равная ингибирующая активность выявлена для экстрактов чайных композиций на основе черного чая с добавлением травы тимьяна алтайского и специи гвоздики или с добавлением семян тмина и корня дягеля; экстрактов готовых чайных композиций на основе зеленого чая из Китая с добавлением листьев мяты перечной и цветов лаванды или с добавлением порошка кожуры апельсина и семян аниса, а также для экстрактов индивидуального растительного сырья, используемого для составления чайных композиций – это черные чаи из Индии, Аргентины, Вьетнама и Шри‐Ланка, листья мяты перечной и цветы лаванды, лишайник цетрария, зеленый чай и ягоды клюквы.

Заключение

Заключение. Полученные результаты позволяют предположить, что чайные композиции на основе черного и зеленого чая, с добавлением различного растительного сырья в виде обычного напитка могут быть полезны людям при инфекции COVID‐19.

Aim

Aim. In vitro analysis of the inhibitory activity of aqueous extracts of ready‐made tea compositions and their constituent ingredients on SARSCOV‐ 2 replication.

Material and Methods

Material and Methods. The laboratory strain SARS‐CoV‐ 2/human/RUS/Nsk‐FRCFTM‐1/2020 was passivated on Vero cell culture. For the preparation of water extracts six types of experimental tea compositions (not containing flavorings) based on black and green tea or flowers of hibiscus as well as individual ingredients were used. Antiviral (inhibitory) activity of plant raw materials was studied in vitro according to the classical scheme of neutralization (inactivation) of the virus.

Results

Results. When compared with the main control sample of an aqueous extract of chaga (Inonotus obliquus) with a 50% effective concentration equal to 13.72±2.99 μg/ml against 103 TCPD50/ml of SARS‐CoV‐2, superior activity was found for extracts of the spice Syzygium aromaticum L. and black tea from Nepal. Approximately equal inhibitory activity was detected for extracts of tea compositions based on black tea with the addition of the grass Thymus altaicus and the spice S. aromaticum L. or with the addition of seeds of Carum carvi L. and the root of Angelica archangelica L. In addition, such activity was detected for extracts of tea compositions based on green tea from China with the addition of leaves of Mentha piperita L. and flowers of Lavandula angustifolia MILL. or with the addition of the peel of Citrus sinensis L. and seeds of Pimpinella anisum L. Extracts from individual plant raw materials used to make tea compositions were black teas from India, Argentina, Vietnam and Sri Lanka, leaves of M. piperita L. and flowers of L. angustifolia MILL. Cetraria islandica lichen, green tea and berries of Oxycoccus also showed antiviral activity.

Conclusion

Conclusion. The results obtained suggest that tea compositions based on black and green tea with the addition of various plant raw materials in the form of a regular drink can be useful to people with COVID‐19 infection.  

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The authors declare that there are no conflicts of interest present.