ecodagЮг России: экология, развитиеSouth of Russia: ecology, development1992-10982413-0958State Institute of Applied Ecology of the Republic of Dagestan10.18470/1992-1098-2020-4-161-167ecodag-2060Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF PRESENTATIONSКлинико-иммунологические сопоставления результатов тестирования на COVID-19 сотрудников поликлиники крупного промышленного центра в период пандемииClinical and immunological comparisons of the results of testing for COVID-19 of employees of an outpatient medical organization of a large industrial centre during a pandemichttps://orcid.org/0000-0002-9834-1799ОбуховаО. О.ObukhovaO. O.

Ольга О. Обухова - доктор медицинских наук, ведущий научный сотрудник Лаборатории иммунологии.630117 Новосибирск, ул. Тимакова, 2.Тел. +79139403293

Olga O. Obukhova - Doctor of Medical Science, Laboratory of Immunology, Federal Research Centre of Fundamental and Translational Medicine.2 Timakova St, Novosibirsk, 630117.Tel. +79139403293

trio188@yandex.ruhttps://orcid.org/0000-0002-7592-8984ТруновА. Н.TrunovA. N.

Александр Н. Трунов.Новосибирск.

Alexander N. Trunov.Novosibirsk.

https://orcid.org/0000-0002-5672-9234ГорбенкоО. М.GorbenkoO. M.

Ольга М. Горбенко.Новосибирск.

Olga M. Gorbenko.Novosibirsk.

https://orcid.org/0000-0002-8247-7085ШваюкА. П.ShvayukA. P.

Аля П. Шваюк.Новосибирск.

Alya P. Shvayuk.Novosibirsk.

https://orcid.org/0000-0002-0990-0078РябиченкоТ. И.RyabichenkoT. I.

Татьяна И. Рябиченко.Новосибирск.

Tatyana I. Ryabichenko.Novosibirsk.

https://orcid.org/0000-0001-5477-3445СкосыреваГ. А.SkosyrevaG A.

Галина А. Скосырева.Новосибирск.

Galina А. Skosyreva.Novosibirsk.

https://orcid.org/0000-0002-1948-9745ОбуховА. В.ObukhovA. V.

Александр В. Обухов.Новосибирск.

Alexander V. Obukhov.Novosibirsk.

Федеральный исследовательский центр фундаментальной и трансляционной медициныFederal Research Centre of Fundamental and Translational MedicineГородская клиническая поликлиника №13City Clinical Polyclinic №13202017012021154161167Copyright © Обухова О.О., Трунов А.Н., Горбенко О.М., Шваюк А.П., Рябиченко Т.И., Скосырева Г.А., Обухов А.В., 20212021Обухова О.О., Трунов А.Н., Горбенко О.М., Шваюк А.П., Рябиченко Т.И., Скосырева Г.А., Обухов А.В.Obukhova O.O., Trunov A.N., Gorbenko O.M., Shvayuk A.P., Ryabichenko T.I., Skosyreva G.A., Obukhov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ecodag.elpub.ru/ugro/article/view/2060

Цель. Определить наличие/отсутствие специфических антител класса IgG к антигенам SARS-CoV-2 у сотрудников амбулаторно-поликлинического звена и проведение клинико-иммунологических сопоставлений за период с апреля по август 2020 года.Материал и методы. Обследовано 386 сотрудников поликлиники г. Новосибирска. Определение IgG-антител к антигенам SARS-CoV-2 в сыворотке крови осуществляли с помощью метода ИФА. Выделение РНК SARS-CoV-2 из соскоба из носоглотки и ротоглотки проводили с использованием ПЦР-диагностики.Результаты. Специфические IgG-антитела к антигенам SARS-CoV-2 были выявлены у 32 (8,42%) сотрудников поликлиники. У 91,58% сотрудников антитела не были выявлены. В группе сотрудников с наличием антител у 9 человек (28,12%) наблюдались клинические симптомы заболевания разной степени тяжести. В этой группе у 4 человек (12,51%) диагностирована двусторонняя внебольничная пневмония с признаками, характерными для COVID-инфекции. Еще у 5 сотрудников (15,61%) с наличием антител имели место признаки ОРВИ легкой и средней степени тяжести. РНК SARS-CoV-2 в группе сотрудников с наличием антител и клиническими проявлениями не была выявлена ни в одном случае. У 23 (71,88%) сотрудников с наличием IgG-антител клинических проявлений заболевания не наблюдалось.Заключение. Наличие специфических антител к SARS-CoV-2 у сотрудников с клиническими проявлениями COVID-19 без обнаружения РНК вируса является ретроспективным подтверждением этиологии перенесенной инфекции. Обнаружение специфических антител у лиц без клинических проявлений может служить подтверждением бессимптомного течения перенесенной коронавирусной инфекции.

Aim. To determine the presence or absence of specific IgG antibodies against SARS-CoV-2 antigens in outpatient clinic staff and to compare clinical and immunological features from April to August 2020.Materials and Methods. The control group comprised 386 employees of the Novosibirsk City Clinical Polyclinic №13.The determination of IgG antibodies against SARS-CoV-2 antigens in blood serum was performed by using the ELISA method. A real time method of reverse transcription and polymerase chain reaction was used to extract RNA SARS-CoV-2 from nasopharyngeal and oropharyngeal swabs.Results. Specific IgG antibodies against SARS-CoV-2 antigens were detected in 32 (8.42%) employees of the polyclinic. Antibodies were not detected in 91.58% of employees. 9 members (28.12%) had clinical symptoms of varying degrees of disease severity in the group of employees with the presence of antibodies, 4 members of this group (12,51%) had bilateral community-acquired pneumonia with signs of COVID infection, another 5 members (15.61%) with antibodies had signs of ARVI of mild and moderate severity. RNA SARS-CoV-2 in the group of employees with the presence of antibodies and clinical implications was not detected in any case; 23 (71.88%) members with the presence of IgG-antibodies did not have clinical implications of the disease.Conclusion. The presence of specific antibodies against SARS-CoV-2 in employees with clinical implications of COVID-19 without detection of the RNA virus in the biological material is a retrospective confirmation of the etiology of the transferred infection. The detection of specific antibodies in persons who did not have clinical implications can serve as confirmation of the asymptomatic course of the transferred coronavirus infection.

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The authors declare that there are no conflicts of interest present.