<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ecodag</journal-id><journal-title-group><journal-title xml:lang="ru">Юг России: экология, развитие</journal-title><trans-title-group xml:lang="en"><trans-title>South of Russia: ecology, development</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1992-1098</issn><issn pub-type="epub">2413-0958</issn><publisher><publisher-name>State Institute of Applied Ecology of the Republic of Dagestan</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18470/1992-1098-2020-1-118-129</article-id><article-id custom-type="elpub" pub-id-type="custom">ecodag-1873</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЭКОЛОГИЧЕСКАЯ ТОКСИКОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ECOLOGICAL TOXICOLOGY</subject></subj-group></article-categories><title-group><article-title>Исследование визуальных патологий у рыб Юго‐Западного побережья Черного моря</article-title><trans-title-group xml:lang="en"><trans-title>The Study of External Pathologies in Fish of the South‐Western Coast of the Black Sea</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9102-0861</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гаврюсева</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Gavruseva</surname><given-names>T. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Татьяна В. Гаврюсева, кандидат биоло‐ гических наук, старший научный сотруд‐ ник отдела ихтиологии</p><p>299011, г. Севастополь, пр. Нахимова, 2. Тел. +7(8692)544110</p></bio><bio xml:lang="en"><p>Tatiana V. Gavruseva, Cand. Biol. Sci., Senior Researcher, Department of Ich‐ thyology</p><p>2 Nakhimov Ave, Sevastopol, Crimea, 299011 Russia. Теl. +7(8692)544110</p></bio><email xlink:type="simple">gavrt2004@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный исследовательский центр «Институт биологии южных морей» имени А.О. Ковалевского РАН»<country>Россия</country></aff><aff xml:lang="en">A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>15</day><month>04</month><year>2020</year></pub-date><volume>15</volume><issue>1</issue><fpage>118</fpage><lpage>129</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Гаврюсева Т.В., 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">Гаврюсева Т.В.</copyright-holder><copyright-holder xml:lang="en">Gavruseva T.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://ecodag.elpub.ru/ugro/article/view/1873">https://ecodag.elpub.ru/ugro/article/view/1873</self-uri><abstract><sec><title>Цель</title><p>Цель. Изучить возможность использования визуальных признаков патологии у рыб для выявления наиболее чувствительных мониторных видов, которые можно использовать для оценки экологического состояния прибрежных акваторий Севастополя.</p></sec><sec><title>Материал и методы</title><p>Материал и методы. Внешнему осмотру и патологоанатомическому вскрытию подвергли 461 экз. одиннадцати массовых видов рыб, отловленных из акватории Севастополя в 2018 г. Для оценки состояния организма рыб на основе клинического и патологоанатомического обследования проведена полуколичественная градация выявленных патологий с использованием индекса оценки здоровья (HAI).</p></sec><sec><title>Результаты</title><p>Результаты. Отмечали повреждения кожных покровов, жабр, альтерации плавников, скелетные деформации, патологии печени, селезенки, гонад, паразитарные инвазии. Всего зафиксировано 23 визуально определяемых патологических изменений. Анализ обнаруженных аномалий с использованием индекса оценки здоровья (HAI) показал, что наиболее показательными биомониторными видами были придонные и донные рыбы: бычок‐мартовик Mesogobius batrachocephalus Pallas, 1814, бычок‐кругляк Neogobius melanostomus Pallas, 1814 и зеленушка рулена Symphodus tinca Linnaeus, 1758. У этих трех видов рыб были выявлены альтерации печени, что может свидетельствовать о хроническом воздействии ксенобиотиков, и эктопаразиты, что указывает на ослабление иммунного статуса исследуемых особей.</p></sec><sec><title>Выводы</title><p>Выводы. Патоморфологические исследования рыб в прибрежной зоне Севастополя показали, что состояние органов и тканей связано с особенностями экологии и чувствительностью исследуемых рыб к загрязнению среды обитания. Результаты исследования представляют интерес при разработке монито‐ринговых программ биотестирования и биоиндикации гидробионтов с помощью биомаркеров состояния здоровья рыб.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Aim</title><p>Aim. The aim of our investigation was to study the possibility of using the visual pathologies of fish as biomarkers of pollution to identify those fish species most sensitive in this way as biological monitors in assessing the ecological state of Sevastopol waters.</p></sec><sec><title>Material and Methods</title><p>Material and Methods. 461 specimens of eleven common species of fish from the waters of Sevastopol were subjected to external examination and dissection in 2018. A semi‐quantitative gradation of the identified pathologies was conducted using the Health Assessment Index (HAI).</p></sec><sec><title>Results</title><p>Results. Twenty three types of visual pathology were recorded. Abnormalities in the skin and gills, fin alterations, skeletal deformities, pathologies of liver, spleen and gonads and parasitic invasions were noted. Analysis of external anomalies using the Health Assessment Index (HAI) revealed that the most potentially indicative species for visual biomonitoring were the following demersal fishes: knout goby Mesogobius batrachocephalus Pallas, 1814, round goby Neogobius melanostomus Pallas, 1814 and East Atlantic peacock wrasse Symphodus tinca Linnaeus, 1758. Liver changes detected in these three fish species may indicate chronic exposure and reaction to xenobiotics and the presence of ectoparasites would indicate a weakening of the immune status of the individual specimens studied.</p></sec><sec><title>Conclusions</title><p>Conclusions. Pathological studies of fish in Sevastopol’s coastal zone indicated that the condition of their organs and tissues is related to environmental factors and that there are sensitivities to habitat pollution. The results of the study are of interest in the future development of monitoring programs for bioassays and bioindication of hydrobionts using fish as biomarkers.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Визуальные патологии</kwd><kwd>Mesogobius batrachocephalus</kwd><kwd>Neogobius melanostomus</kwd><kwd>Symphodus tinca</kwd><kwd>мониторинг</kwd><kwd>биоиндикаторы</kwd><kwd>индекс здоровья рыб</kwd><kwd>загрязнение</kwd><kwd>бухты Севастополя</kwd></kwd-group><kwd-group xml:lang="en"><kwd>External pathology</kwd><kwd>Mesogobius batrachocephalus</kwd><kwd>Neogobius melanostomus</kwd><kwd>Symphodus tinca</kwd><kwd>monitoring</kwd><kwd>bioindicators</kwd><kwd>Health Assessment Index (HAI)</kwd><kwd>pollution</kwd><kwd>Sevastopol bay</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Работа выполнена по теме государственного задания Федеральный исследовательский центр «Институт биологии южных морей имени А.О. Ковалевского РАН» «Функциональные, метаболические и токсикологиче‐ские аспекты существования гидробионтов и их популяций в биотопах с различным физико‐химическим режимом», номер гос. регистрации АААА–А18–118021490093–4; «Закономерности формирования и антропогенная трансформация биоразнообразия и биоресурсов Азово‐Черноморского бассейна и других районов Мирового океана», номер гос. регистрации АААА‐А18‐118020890074‐2.</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>The work was carried out according to the state assignment tasks of the Federal Research Centre, A.O. Kovalev‐ sky Institute of Biology of the South Seas, Russian Academy of Sciences: “Functional, metabolic and toxicological aspects of the existence of hydrobionts and their populations in biotopes with differing physico‐chemical regimes”, state registration number AAAA–A18–118021490093–4; and “Patterns of formation and anthropogenic transformation of biodiversity and biological resources of the Azov‐Black Sea basins and other regions of the world ocean”, state registration number AAAA‐A18‐118020890074‐2.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Копытов Ю.П., Минкина Н.И., Самышев Э.З. Уровень загрязненности воды и донных отложений Севастопольской бухты (Черное море) // Системы контроля окружающей среды. Средства, модели и мониторинг: Сб. науч. тр. НАН Украины. МГИ: Севастополь, 2010. С. 199‐208. URL: http://www.nbuv.gov.ua/Portal/natural/Skns/2010/articles (дата обращения: 16.01.2019)</mixed-citation><mixed-citation xml:lang="en">Kopytov Yu.P., Minkina N.I., Samyshev E.Z. [Level of water and sediments pollution in the Sevastopol Bay (Black Sea)]. In: Sistemy kontrolya okruzhayushchei sredy. Sredstva, modeli i monitoring [Environmental Control Systems. Means, Models and Monitoring]. Sevastopol', MSI Publ., 2010, pp. 199‐208. (In Russian) Available at: http://www.nbuv.gov.ua/Portal/natural/Skns/2010/articles (accessed 16.01.2019)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Малахова Л.В. Полихлорированные бифенилы и органический углерод в донных отложениях Севастопольской и Балаклавской бухт (Чёрное море) // Морской экологический журнал. 2013. Т. XII. N 1. С. 52‐58.</mixed-citation><mixed-citation xml:lang="en">Malakhova L.V. Polychlorinated biphenyls and organic carbon in bottom sediments of Sevastopol and Balaklava Bays (the Black Sea). Morskoi ekologicheskii zhurnal [Marine Ecological Journal]. 2013, vol. XII, no. 1, pp. 52‐58. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Мезенцева И.В., Мальченко Ю.А. Комплексный подход в организации мониторинга загрязнения морских вод в прибрежных акваториях Севастополя // Труды Государственного Океанографического института. 2015. Вып. 216. С. 326‐339.</mixed-citation><mixed-citation xml:lang="en">Mezenceva I.V., Malchenko Y.A. [Integrated approach in the organization of marine pollution monitoring in coastal water areas of Sevastopol]. In: Trudy Gosudarstvennogo Okeanograficheskogo instituta [Proceedings of State Oceanographic Institute]. 2015, iss. 216, pp. 326‐339. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Khanaychenko А.N., Giragosov V.E., Gaevskaya A.V. Epizootological state of the wild Black sea turbot (kalkan). Grossly visible pathology: preliminary data // Marine Ecological Journal. 2012. V. 11. N 4. P. 85‐94.</mixed-citation><mixed-citation xml:lang="en">Khanaychenko А.N., Giragosov V.E., Gaevskaya A.V. Epizootological state of the wild Black sea turbot (kalkan). Grossly visible pathology: preliminary data. Marine Ecological Journal, 2012, vol. 11, no. 4, pp. 85‐94.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Экотоксикологические исследования прибрежной черноморской ихтиофауны в районе Севастополя / отв. ред. И.И. Руднева; Ин‐т морских биологических исследований им. А.О. Ковалевского РАН; Российский фонд фундаментальных исследований. Москва: ГЕОС, 2016. 360 с.</mixed-citation><mixed-citation xml:lang="en">Rudneva I.I., ed. Ekotoksikologicheskie issledovaniya pribrezhnoi chernomorskoi ikhtiofauny v raione Sevastopolya [Ecotoxicological Studies of Coastal Black Sea Ichthyofauna in the Region of Sevastopol]. Moscow, GEOS Publ., 2016, 360 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Kovyrshina T.B., Rudneva I.I. The Response of Blood Biomarkers of the Round Goby Neogobius melanostomus (Pallas, 1814) (Perciformes: Gobiidae) to Chronic Coastal Pollution in the Sea of Azov // Russian Journal of Marine Biology. 2018. V. 44. P. 328‐333. DOI: 10.1134/S1063074018040065</mixed-citation><mixed-citation xml:lang="en">Kovyrshina T.B., Rudneva I.I. The Response of Blood Biomarkers of the Round Goby Neogobius melanostomus (Pallas, 1814) (Perciformes: Gobiidae) to Chronic Coastal Pollution in the Sea of Azov. Russian Journal of Marine Biology, 2018, vol. 44, pp. 328‐333. DOI: 10.1134/S1063074018040065</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Au D.W.T. The application of histo‐cytopathological biomarkers in marine pollution monitoring: a review // Marine Pollution Bulletin. 2004. V. 48. Iss. 9‐10. P. 817‐834. DOI: 10.1016/j.marpolbul.2004.02.032</mixed-citation><mixed-citation xml:lang="en">Au D.W.T. The application of histo‐cytopathological biomarkers in marine pollution monitoring: a review. Marine Pollution Bulletin, 2004, vol. 48, iss. 9‐10, pp. 817‐834. DOI: 10.1016/j.marpolbul.2004.02.032</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Моисеенко Т.И. Водная токсикология. Теоретические и прикладные аспекты. Москва: Наука, 2009. 400 с.</mixed-citation><mixed-citation xml:lang="en">Moiseenko T.I. Vodnaya toksikologiya. Teoreticheskie i prikladnye aspekty [Aquatic Toxicology. Theoretical and Applied Aspects]. Moscow, Nauka Publ., 2009, 400 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Bucke D., Vethaak A.D., Lang T., Mellergaard S. Common Diseases and Parasites of Fish in the North Atlantic: Training Guide for Identification // ICES Techniques in Marine Environmental Sciences. 1996. N 19. 27 p. DOI: 10.25607/OBP‐255</mixed-citation><mixed-citation xml:lang="en">Bucke D., Vethaak A.D., Lang T., Mellergaard S. Common Diseases and Parasites of Fish in the North Atlantic: Training Guide for Identification. ICES Techniques in Marine Environmental Sciences, 1996, no. 19, 27 p. DOI: 10.25607/OBP‐255</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Feist S.W., Lang T. Liver tumours in flatfish // ICES Identification Leaflets for Diseases and Parasites of Fish and Shellfish. 2014. Iss. 61. 5 p. DOI: 10.17895/ices.pub.5242</mixed-citation><mixed-citation xml:lang="en">Feist S.W., Lang T. Liver tumours in flatfish. ICES Identification Leaflets for Diseases and Parasites of Fish and Shellfish, 2014, iss. 61, 5 p. DOI: 10.17895/ices.pub.5242</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Новак А.И. Инвазии рыб в водоемах с различными экологическими условиями // Российский паразитологический журнал. 2010. N 2. С. 6‐10.</mixed-citation><mixed-citation xml:lang="en">Novak A.I. Invasions of fishes in pools with different ecological conditions. Rossiiskii parazitologicheskii zhurnal [Russian Parasitological Journal]. 2010, no. 2, pp. 6‐10. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Sures B., Nachev M., Selbach C., Marcogliese D.J. Parasite responses to pollution: what we know and where we go in ‘Environmental Parasitology’ // Parasites and Vectors. 2017. V. 10(65). P. 1‐19. DOI 10.1186/s13071‐017‐2001‐3</mixed-citation><mixed-citation xml:lang="en">Sures B., Nachev M., Selbach C., Marcogliese D.J. Parasite responses to pollution: what we know and where we go in ‘Environmental Parasitology’. Parasites and Vectors, 2017, vol. 10(65), pp. 1‐19. DOI 10.1186/s13071‐017‐2001‐3</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Решетников Ю.С., Акимова Н.В., Попопа О.А. Аномалии в системе воспроизводства рыб при антропогенном воздействии // Известия Самарского научного центра Российской академии наук. 2000. Т. 2. N 2. С. 274‐282.</mixed-citation><mixed-citation xml:lang="en">Reshetnikov Y.S., Akimova N.V., Popopa O.A. Morphological anomalies in the reproduction system of fish under pollution. Izvestiya Samarskogo nauchnogo tsentra Rossiiskoi akademii nauk [Izvestia of Samara Scientific Center of the Russian Academy of Sciences]. 2000, vol. 2, no. 2, pp. 274‐282. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Госькова О.А., Мельниченко И.П., Богданов В.Д. Морфологические аномалии и травмы у пеляди в период нерестовой миграции в уральских притоках Оби // Вестник АГТУ. Сер. Рыбное хозяйство. 2014. N 4. С. 7‐15.</mixed-citation><mixed-citation xml:lang="en">Goskova O.A., Melnichenko I.P., Bogdanov V.D. Morphological anomalies and traumas of peled during spawning migration in the Ural Ob tributaries. Vestnik Astrakhanskogo gosudarstvennogo tekhnicheskogo universiteta. Seriya: Rybnoye khozyaystvo [Vestnik of Astrakhan State Technical University. Series: Fishing industry]. 2014, no. 4, pp. 7‐15. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Adams S.M., Brown A.M., Goede R.W. A Quantitative Health Assessment Index for Rapid Evaluation of Fish Condition in the Field // Transactions of the American Fisheries Society. 1993. V. 122. Iss. 1. P. 63‐73. DOI: 10.1577/1548‐8659(1993)122&lt;0063:AQHAIF&gt;2.3.CO;2</mixed-citation><mixed-citation xml:lang="en">Adams S.M., Brown A.M., Goede R.W. A Quantitative Health Assessment Index for Rapid Evaluation of Fish Condition in the Field. Transactions of the American Fisheries Society, 1993, vol. 122, iss. 1, pp. 63‐73. DOI: 10.1577/1548‐8659(1993)122&lt;0063:AQHAIF&gt;2.3.CO;2</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Kumar D.M. Application of the health assessment index (HAI) for rapid evaluation of fish health // Journal of the Inland Fisheries Society of India. 2005. V. 37. Iss. 2. P. 87‐92.</mixed-citation><mixed-citation xml:lang="en">Kumar D.M. Application of the health assessment index (HAI) for rapid evaluation of fish health. Journal of the Inland Fisheries Society of India, 2005, vol. 37, iss. 2, pp. 87‐92.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Чеснокова И.И. Активность аминотрансфераз и щелочной фосфатазы в гонадах черноморских бычкакругляка и бычка‐мартовика // Вестник Тюменского государственного университета. Экология и природопользование. 2017. Т. 3. N3. С. 114‐128. DOI: 10.21684/2411‐7927‐2017‐3‐3‐114‐128</mixed-citation><mixed-citation xml:lang="en">Chesnokova I.I. Activity of aminotransferases and alkaline phosphatase in the gonads of round goby and knout goby. Tyumen State University Bulletin. Natural Resource Use and Ecology, 2017, vol. 3, no. 3, pp. 114‐128. DOI: 10.21684/2411‐7927‐2017‐3‐3‐114‐128 (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Овен Л.С. Специфика развития половых клеток морских рыб в период размножения как показатель типа нереста и реакции на условия среды обитания. М.: Изд-во ВНИРО, 2004. 188 с.</mixed-citation><mixed-citation xml:lang="en">Oven L.S. Spetsifika razvitiya polovykh kletok morskikh ryb v period razmnozheniya kak pokazatel' tipa neresta i reaktsii na usloviya sredy obitaniya [The specifics of the development of gametes of marine fish during the breeding season as an indicator of the type of spawning and response to environmental conditions]. Moscow, VNIRO Publ., 2004, 188 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Селюков А.Г., Чернов В.С., Жуков Г.В., Шуман Л.А. Гистопатологические изменения жаберного аппарата черноморской скорпены Scorpaena porcus // Сборник статей Всероссийской научной конференции с международным участием, посвященной 125‐летию профессора В. А. Водяницкого «Загрязнение морской среды: экологический мониторинг, биоиндикация, нормирование», Севастополь, 28 мая‐1 июня, 2018. С. 228‐233.</mixed-citation><mixed-citation xml:lang="en">Selyukov A.G., Chernov V.S., Zhukov G.V., Shuman L.A. Gistopatologicheskie izmeneniya zhabernogo apparata chernomorskoi skorpeny Scorpaena porcus [Histopathological changes of the gill apparatus of the Black Sea scorpion fish Scorpaena porcus]. Sbornik statei Vserossiiskoi nauchnoi konferentsii s mezhdunarodnym uchastiem, posvyashchennoi 125‐letiyu professora V. A. Vodyanitskogo «Zagryaznenie morskoi sredy: ekologicheskii monitoring, bioindikatsiya, normirovanie», Sevastopol', 28 maya‐1 iyunya, 2018 [Procedures of the All‐Russian Scientific Conference with international participation dedicated to the 125th anniversary of Professor V. A. Vodyanitsky “Pollution of the marine environment: environmental monitoring, bioindication and rationing”, Sevastopol, May 28‐June 1 2018]. Sevastopol, 2018, pp. 228‐233. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Корнийчук Ю.М., Мартыненко И.М. Особенности распределения метацеркарий трематод рода Cryptocotyle (Heterophyidae) по поверхности тела бычка‐кругляка Apollonia (Neogobius) melanostomus // Экология моря. 2009. Вып. 79. С. 11‐14.</mixed-citation><mixed-citation xml:lang="en">Korniychuk Yu.M., Martinenko I.M. Some peculiarities of Cryptocotyle metacercariae (Heterophyidae) distribution on the body surface of the round goby, Apollonia (Neogobius) melanostomus. Ekologiya morya [Marine Ecological Journal]. 2009, vol. 79, pp. 11‐14. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Юрахно В.М., Горчанок Н.В. Миксоспоридия Kudoa nova (Myxosporea: Kudoidae) – паразит рыб Чёрного и Азовского морей // Морской экологический журнал. 2011. Т. X. N 2. С. 68‐77.</mixed-citation><mixed-citation xml:lang="en">Yurakhno V.M., Gorchanok N.V. Myxosporea Kudoa nova (Myxosporea: Kudoidae) – parasite of Black Sea and Sea of Azov fish. Morskoi ekologicheskii zhurnal [Marine Ecological Journal]. 2011, vol. X, no. 2, pp. 68‐77. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Гаевская А.В. Паразиты и болезни рыб Черного и Азовского морей: I ‐ морские, солоноватоводные и проходные рыбы. Севастополь: ЭКОСИГидрофизика, 2012. 380 с.</mixed-citation><mixed-citation xml:lang="en">Gaevskaya A.V. Parazity i bolezni ryb Chernogo i Azovskogo morei: I ‐ morskie, solonovatovodnye i prokhodnye ryby [Parasites and Fish Diseases of the Black and Azov Seas: I ‐ Marine, Brackish and Migratory Fish]. Sevastopol, EKOSIGidrofizika Publ., 2012, 380 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Болтачёв А.Р., Карпова Е.П. Ихтиофауна прибрежной зоны Севастополя (Чёрное море) // Морской экологический журнал. 2012. Т. 11. N 2. С. 10‐27.</mixed-citation><mixed-citation xml:lang="en">Boltachev A.R., Karpova E.P. The ichthyofauna of the Sevastopol coastal zone (the Black Sea) Sea). Morskoi ekologicheskii zhurnal [Marine Ecological Journal]. 2012, vol. 11, no. 2, pp. 10‐27. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Чернышева Н.Б., Кузнецова Е.В., Воронин В.Н., Стрелков Ю.А. Паразитологическое исследование рыб. Методическое пособие. СПб.: ГОСНИОРХ. 20 с.</mixed-citation><mixed-citation xml:lang="en">Chernysheva N.B., Kuznetsova E.V., Voronin V.N., Strelkov Yu.A. Parazitologicheskoe issledovanie ryb [Parasito‐logical study of fish]. St. Petersburg, GOSNIORH Publ., 2009, 20 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Noga E.J. Fish Disease: Diagnosis and Treatment. Wiley‐Blackwell; 2nd ed., 2010. 536 p.</mixed-citation><mixed-citation xml:lang="en">Noga E.J. Fish Disease: Diagnosis and Treatment. Wiley‐Blackwell; 2nd ed., 2010, 536 p.</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Berillis P. Factors that can lead to the development of skeletal deformities in fishes: a review // Journal of Fisheries Sciences.com. 2015. V. 9. Iss. 3. P. 17‐23.</mixed-citation><mixed-citation xml:lang="en">Berillis P. Factors that can lead to the development of skeletal deformities in fishes: a review. Journal of Fisheries Sciences.com, 2015, vol. 9, iss. 3, pp. 17‐23.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Myers M.S. Anulacion B.F., French B.L., Reichert W.L., Laetz C.A., Buzitis J., Olson O.P., Sol S., Collier T.K. Improved flatfish health following remediation of a PAH‐contaminatedsite in Eagle Harbor, Washington // Aquatic Toxicology. 2008. V. 88. Iss. 4. P. 277‐288. DOI: 10.1016/j.aquatox.2008.05.005</mixed-citation><mixed-citation xml:lang="en">Myers M.S. Anulacion B.F., French B.L., Reichert W.L., Laetz C.A., Buzitis J., Olson O.P., Sol S., Collier T.K. Improved flatfish health following remediation of a PAH‐contaminated site in Eagle Harbor, Washington. Aquatic Toxicology, 2008, vol. 88, iss. 4, pp. 277‐288. DOI: 10.1016/j.aquatox.2008.05.005</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Гаврюсева Т.В., Сергеенко Н.В., Устименко Е.А., Рязанова Т.В. и др. Патогены гидробионтов Камчатки // Исследования водных биологических ресурсов Камчатки и северо‐западной части Тихого океана. Петропавловск‐Камчатский: КамчатНИРО. 2012. Вып. 25. С. 190‐207.</mixed-citation><mixed-citation xml:lang="en">Gavryuseva T.V., Sergeenko N.V., Ustimenko E.A., Ryazanova T.V. et al. Pathogens of Kamchatka's hydrobionts. In: Issledovaniya vodnykh biologicheskikh resursov Kamchatki i severo‐zapadnoy chasti Tikhogo okeana [Researches into the aquatic biological resources of Kamchatka and the north-west part of the Pacific Ocean]. 2014, vol. 25, pp. 190‐207. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Vidal Martinez V.M. Helminths and protozoans of aquatic organisms as bioindicators of chemical pollution // Parassitologia. 2007. V. 49. Iss. 3. P. 177‐184.</mixed-citation><mixed-citation xml:lang="en">Vidal Martinez V.M. Helminths and protozoans of aquatic organisms as bioindicators of chemical pollution. Parassitologia, 2007, vol. 49, iss. 3, pp. 177‐184.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Skuratovskaya E.N., Kovyrshina T.B., Chesnokova I.I. Influence of Cryptocotyle spp. metacercariae (Creplin, 1825) on some biochemical parameters of the round goby Neogobius melanostomus (Pallas, 1814) // Bulletin of the European Association of Fish Pathologists. 2019. V. 39. Iss. 1. P. 24‐30.</mixed-citation><mixed-citation xml:lang="en">Skuratovskaya E.N., Kovyrshina T.B., Chesnokova I.I. Influence of Cryptocotyle spp. metacercariae (Creplin, 1825) on some biochemical parameters of the round goby Neogobius melanostomus (Pallas, 1814). Bulletin of the EuropeanAssociation of Fish Pathologists, 2019, vol. 39, iss. 1, pp. 24‐30.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
