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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ecodag</journal-id><journal-title-group><journal-title xml:lang="ru">Юг России: экология, развитие</journal-title><trans-title-group xml:lang="en"><trans-title>South of Russia: ecology, development</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1992-1098</issn><issn pub-type="epub">2413-0958</issn><publisher><publisher-name>State Institute of Applied Ecology of the Republic of Dagestan</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18470/1992-1098-2019-2-189-201</article-id><article-id custom-type="elpub" pub-id-type="custom">ecodag-1615</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КРАТКИЕ СООБЩЕНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BRIEF PRESENTATIONS</subject></subj-group></article-categories><title-group><article-title>ВЛИЯНИЕ АЗОТНЫХ И ГУМИНОВЫХ УДОБРЕНИЙ НА БИОХИМИЧЕСКОЕ СОСТОЯНИЕ НЕФТЕЗАГРЯЗНЕННОГО ЧЕРНОЗЕМА</article-title><trans-title-group xml:lang="en"><trans-title>EFFECT OF NITROGEN AND HUMIC FERTILIZERS ON THE BIOCHEMICAL STATE OF OIL‐CONTAMINATED CHERNOZEM</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Минникова</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Minnikova</surname><given-names>T. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат биологических  наук, младший  научный  сотрудник  кафедры  экологии  и  природопользования  Академии  биологии  и  биотехнологии  им.  Д.И.  Ивановского,</p><p>просп.  Стачки  194/1, г. Ростов‐на‐Дону, 344090 </p></bio><bio xml:lang="en"><p>Cand. Sci. (Biol.), Junior Researcher, Department of Ecology and Nature Management, Academy of Biology and Biotechnology named after D.I.Ivanovsky, </p><p>194/1 Stachky ave., Rostov‐on‐Don, 344090</p></bio><email xlink:type="simple">loko261008@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Колесников</surname><given-names>С. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Kolesnikov</surname><given-names>S. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор  сельскохозяйственных  наук,  профессор,  зав.  кафедрой экологии и природопользования Академии биологии и биотехнологии им. Д.И. Ивановского,</p><p>г. Ростов‐на‐Дону</p></bio><bio xml:lang="en"><p>Dr. Sci. (Agric.), Professor, Head of the Department for Ecology and Nature Management, Academy of Biology and Biotechnology named after D.I.Ivanovsky, </p><p>Rostov‐on‐Don</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Денисова</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Denisova</surname><given-names>T. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор  биологических наук, профессор кафедры экологии и  природопользования  Академии  биологии  и  биотехнологии  им.  Д.И.  Ивановского,</p><p>г. Ростов‐на‐Дону</p></bio><bio xml:lang="en"><p>Dr. Sci. (Biol.), Professor, Department of Ecology and Nature Management, Academy of Biology and Biotechnology named after D.I. Ivanovsky,</p><p>Rostov‐on‐Don</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Южный федеральный университет<country>Россия</country></aff><aff xml:lang="en">Southern Federal University<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>05</day><month>07</month><year>2019</year></pub-date><volume>14</volume><issue>2</issue><fpage>189</fpage><lpage>201</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Минникова Т.В., Колесников С.И., Денисова Т.В., 2019</copyright-statement><copyright-year>2019</copyright-year><copyright-holder xml:lang="ru">Минникова Т.В., Колесников С.И., Денисова Т.В.</copyright-holder><copyright-holder xml:lang="en">Minnikova T.V., Kolesnikov S.I., Denisova T.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://ecodag.elpub.ru/ugro/article/view/1615">https://ecodag.elpub.ru/ugro/article/view/1615</self-uri><abstract><sec><title>Цель</title><p>Цель. Оценить влияние  азотных и  гуминовых  удобрений  на  биохимическое  состояние  нефтезагрязненного  чернозема.</p></sec><sec><title>Методы</title><p>Методы. Использовали  для  моделирования  нефтезагрязнения чернозема дозы нефти 1, 5 и 10% от массы почвы. Срок экспозиции составил 30, 60 и 90 суток. Для моделирования биоремедиации нефтезагрязненного чернозема использовали гумат калия и натрия, мочевину и нитроаммофос. Азотные удобрения  вносили в почву с целью восполнения равновесия между углеродом и азотом: мочевину с  содержанием азота 46%, нитроаммофос с содержанием азота 15%. Гуминовые удобрения  –  гумат  калия и  гумат натрия – вносили в почву для  стимуляции  аборигенной нефтедеструктивной микробиоты. Для оценки биологической активности почвы определяли: активность каталазы, инвертазы и интенсивность эмиссии СО2.</p></sec><sec><title>Результаты</title><p>Результаты. Изучено влияние мочевины, нитроаммофоса, гумата калия и натрия на ферментативную активность и  эмиссию СО2 чернозема обыкновенного, загрязненного нефтью 1, 5 и 10% от массы почвы  через  90  суток  в  модельном  эксперименте. Активность  каталазы  снижалась  после  внесения нитроаммофоса, а дыхание и активность инвертазы повышались при низких уровнях загрязнения нефтью. Мочевина, внесенная в почву при 10%‐ной дозе нефти, стимулировала активность каталазы. Гуматы калия и натрия оказывали стимулирующее воздействие  на ферментативную активность и выделение углекислого газа при концентрациях нефти 1 и 5%.</p></sec><sec><title>Заключение</title><p>Заключение. Для диагностики состояния черноземов при загрязнении нефтью дозы  5‐10%  после  внесения  азотных  и  гуминовых  мелиорантов,  целесообразно  использовать  интенсивность  выделения  СО2  почвой  и  активность  инвертазы.  При  более  низких  дозах  нефти после внесения  азотных удобрений целесообразно оценивать состояние почвы по  активности каталазы. </p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Aim</title><p>Aim. In this paper, we aim to assess the effect of nitrogen and humic fertilizers on the biochemical state of oil‐contaminated chernozem.</p></sec><sec><title>Methods</title><p>Methods. In order to simulate the oil pollu‐ tion, chernozem was exposed to oil doses constituting 1, 5 and 10% of the soil mass for 30, 60 and 90 days. For simulating bioremediation of oil‐contaminated chernozem, the following fertilizers were used: potassium and sodium humates, urea and nitroammophos. Nitrogen fertilizers – urea and nitroammophos having a nitrogen content of 46% and 15%, respectively – were applied to the soil for the purposes of restoring the equilibrium between carbon and nitrogen. Humic fertilizers (potassium and sodium humates) were applied to the soil for stimulating the indigenous oil destructive microbiota. In order to assess the biological activity of the soil, we determined catalase activity, invertase activity, as well as CO2 emission intensity.</p></sec><sec><title>Results</title><p>Results. The effect of urea, nitroammophos, potassium and sodium humates on the enzymatic activity and CO2 emissions of ordinary chernozem, which had been exposed to various doses of oil (1, 5 and 10% of the soil mass) for 90 days, was studied in a model experiment. Following the introduction of nitroammophos into soil with low levels of oil pollution, catalase activity decreased, whereas respiration and invertase activity increased. Urea introduced into the soil contaminat‐ ed with a 10% dose of oil stimulated catalase activity. At oil concentrations of 1 and 5%, the introduction of potassium and sodium humates had a stimulating effect on enzymic activity and carbon dioxide evolution.</p></sec><sec><title>Conclusions</title><p>Conclusions. It is advisable to use the intensity of CO2 emissions released from the soil, as well as the invertase activity for diagnosing the state of chernozem con‐ taminated with oil (5‐10%) following the introduction of nitrogen and humic ameliorants. At lower doses of oil, it is advisable to assess the state of the soil following the introduction of nitrogen fertilizers by catalase activity. </p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>загрязнение</kwd><kwd>нефть</kwd><kwd>чернозем  обыкновенный</kwd><kwd>биоремедиация</kwd><kwd>активность каталазы</kwd><kwd>активность инвертазы</kwd><kwd>эмиссия СО2</kwd><kwd>модельный эксперимент</kwd></kwd-group><kwd-group xml:lang="en"><kwd>pollution</kwd><kwd>petrol</kwd><kwd>ordinary chernozem</kwd><kwd>bioremediation</kwd><kwd>catalase activity</kwd><kwd>invertase activity</kwd><kwd>CO2 emission</kwd><kwd>model experiment</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Исследование выполнено при поддержке Министерства образования и науки Российской Федерации (5.5735.2017/8.9) и Президента Российской Федерации (НШ‐ 3464.2018.11).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Peng R.H., Xiong A.S., Xeo Y., Fu X.Y., Gao F., Zhao W., Tian Y.S., Yao, Q.H. Microbial bio‐ degradation of polyaromatic hydrocarbons: a review // FEMS Microbiol Reviews. 2008. V. 32. P. 927‐955. Doi:10.1111/j.1574‐6976.2008.00127.x</mixed-citation><mixed-citation xml:lang="en">Peng R.H., Xiong A.S., Xeo Y., Fu X.Y., Gao F., Zhao W., Tian Y.S., Yao, Q.H. Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Reviews, 2008, vol. 32, pp. 927‐955. Doi: 10.1111/j.1574‐6976.2008.00127.x</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Kimes N.E., Callaghan A.V., Suflita J.M., Morris P.J. Microbial transformation of the Deep‐ water Horizon oil spill – past, present, and future perspectives // Frontiers in Microbiology. 2014. V. 5. 603 p. Doi: 10.3389/fmicb.2014.00603</mixed-citation><mixed-citation xml:lang="en">Kimes N.E., Callaghan A.V., Suflita J.M., Morris P.J. Microbial transformation of the Deepwater Horizon oil spillpast, present, and future perspectives. Frontiers in Microbiology, 2014, no. 5, 603 p. Doi: 10.3389/fmicb.2014.00603</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Riveroll‐Larios J., Escalante‐Espinosa E., Fócil‐Monterrubio R.L., Díaz‐Ramírez I.J. Biological activity assessment in Mexican tropical soils with different hydrocarbon contamination histories // Water Air Soil Pollution. 2015. V. 226. Iss. 10. 353 p. Doi: 10.1007/s11270‐015‐2621‐1</mixed-citation><mixed-citation xml:lang="en">Riveroll‐Larios J., Escalante‐Espinosa E., Fócil‐Monterrubio R.L., Díaz‐Ramírez I.J. Biological activity assessment in Mexican tropical soils with different hydrocarbon contamination histories. Water Air Soil Pollution, 2015, vol. 226, iss. 10, 353 p. Doi: 10.1007/s11270‐015‐2621‐1</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Wolińska A., Kuźniar A., Szafranek‐Nakonieczna A., Jastrzębska N., Roguska E., Stępniew‐ ska Z. Biological activity of autochthonic bacterial community in oil contaminated soil // Water Air Soil Pollution. 2016. V. 227. 130 p. Doi: 10.1007/s11270‐016‐2825‐z</mixed-citation><mixed-citation xml:lang="en">Wolińska A., Kuźniar A., Szafranek‐Nakonieczna A., Jastrzębska N., Roguska E., Stępniewska Z. Biological activity of autochthonic bacterial community in oil contaminated soil. Water Air Soil Pollution, 2016, vol. 227, 130 p. Doi: 10.1007/s11270‐016‐2825‐z</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Nikolopoulou M., Pasadakis N., Norf H., Kalogerakis, N. Enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids // Marine Pollution Bulletin. 2013. V. 77. Iss. 1‐2. P. 37‐44. Doi: 10.1016/j.marpolbul.2013.10.038</mixed-citation><mixed-citation xml:lang="en">Nikolopoulou M., Pasadakis N., Norf H., Kalogerakis, N. Enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids. Marine Pollution Bulletin, 2013, vol. 77, iss. 1‐2, pp. 37‐44. Doi: 10.1016/j.marpolbul.2013.10.038</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Macaulay B.M., Rees D. Bioremediation of oil spills: a review of challenges for research advancement // Annals of Environmental Science. 2014. V. 8. P. 9‐37.</mixed-citation><mixed-citation xml:lang="en">Macaulay B.M., Rees D. Bioremediation of oil spills: a review of challenges for research advancement. Annals of Environmental Science, 2014, vol. 8, pp. 9‐37.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Poi G., Shahsavari E., Aburto‐Medina A., Mok P.Ch., Ball A.S. Large scale treatment of total petroleum‐hydrocarbon contaminated groundwater using bioaugmentation // Journal of Environmental Management. 2018. V. 214. P. 157‐163. Doi: 10.1016/j.jenvman.2018.02.079</mixed-citation><mixed-citation xml:lang="en">Poi G., Shahsavari E., Aburto‐Medina A., Mok P.Ch., Ball A.S. Large scale treatment of total petroleum‐hydrocarbon contaminated groundwater using bioaugmentation. Journal of Environmental Management, 2018, vol. 214, pp. 157‐163. Doi:10.1016/j.jenvman.2018.02.079</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Polyak Y.M., Bakina L.G., Chugunova M.V., Mayachkina N.V., Gerasimov A.O., Bure V.M. Effect of remediation strategies on biological activity of oil‐contaminated soil ‐ A field study // International Biodeterioration &amp; Biodegradation. 2018. V. 126. P. 57‐68. Doi: 10.1016/j.ibiod.2017.10.004</mixed-citation><mixed-citation xml:lang="en">Polyak Y.M., Bakina L.G., Chugunova M.V., Mayachkina N.V., Gerasimov A.O., Bure V.M. Effect of remediation strategies on biological activity of oil‐contaminated soil  ‐ A field study. International Biodeterioration &amp; Biodegradation, 2018, vol. 126, pp. 57‐68. Doi: 10.1016/j.ibiod.2017.10.004</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Шаповалова Н.Н., Годунова Е.И., Менькина Е.А. Влияние последействия применения удобрений на состояние и продуктивность чернозема обыкновенного // Агрохимический вестник. 2018. N 5. С. 9‐15. Doi: 10.24411/0235‐2516‐2018‐10036</mixed-citation><mixed-citation xml:lang="en">Shapovalova N.N., Godunova E.I., Menkina E.A. Influence of fertilizers application aftereffect on ecological and agrochemical state of chernozem ordinary. Agrokhimicheskiy vestnik [Agrochemical Herald]. 2018, no. 5, pp. 9‐15. (In Russian) Doi: 10.24411/0235‐2516‐2018‐10036</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Varjani S.J., Upasani V.N. A new look on factors affecting microbial degradation of petrole‐ um hydrocarbon pollutants // International Biodeterioration &amp; Biodegradation. 2017. V. 120. P. 71‐83. Doi: 10.1016/j.ibiod.2017.02.006</mixed-citation><mixed-citation xml:lang="en">Varjani S.J., Upasani V.N. A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants. International Biodeterioration &amp; Biodegradation, 2017, vol. 120, pp. 71‐ 83. Doi: 10.1016/j.ibiod.2017.02.006</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Киреева Н.А., Новоселова Е.И., Григориади А.С. Влияние загрязнения почв нефтью на физиологические показатели растений и ризосферную микробиоту // Агрохимия. 2009. N 7. С. 71‐80.</mixed-citation><mixed-citation xml:lang="en">Kireeva N.A., Novoselova E.I., Grigoriadi A.S. Effect of soil pollution by oil on the physiological parameters of plants and the rhizosphere microbiota. Agrokhimiya [Agricultural Chemistry]. 2009, no. 7, pp. 71‐80. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Minnikova T.V., Denisova T.V., Kolesnikov S.I., Akimenko Yu.V. Assessment of Agroecological Indicators of Oil‐Contaminated Chernozem in Rostov Oblast after Remediation with Urea and Potassium Humate // Russian Agricultural Sciences. 2018. V. 44. Iss. 2. P. 177‐180. Doi: 10.3103/S106836741802</mixed-citation><mixed-citation xml:lang="en">Minnikova T.V., Denisova T.V., Kolesnikov S.I., Akimenko Yu.V. Assessment of Agroecological Indicators of Oil‐Contaminated Chernozem in Rostov Oblast after Remediation with Urea and Potassium Humate. Russian Agricultural Sciences, 2018, vol. 44, iss. 2, pp. 177‐180. Doi: 10.3103/S1068367418020118</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Wu M., Dick W.A., Li W., Wang X., Yang Q., Wang T., Xu L., Zhang M., Chen L., Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum‐contaminated soil // International Biodeterioration &amp; Biodegradation. 2016. V. 107. P. 158‐164. Doi: 10.1016/j.ibiod.2015.11.019</mixed-citation><mixed-citation xml:lang="en">Wu M., Dick W.A., Li W., Wang X., Yang Q., Wang T., Xu L., Zhang M., Chen L., Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum‐contaminated soil. International Biodeterioration &amp; Biodegradation, 2016, vol. 107, pp. 158‐164. Doi: 10.1016/j.ibiod.2015.11.019</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Габбасова И.М., Сулейманов Р.Р., Гарипов Т.Т. Деградация и мелиорация почв при загрязнении нефтепромысловыми сточными водами // Почвоведение. 2013. N 2. С. 226‐211. Doi: 10.7868/S0032180X13020056</mixed-citation><mixed-citation xml:lang="en">Gabbasova I.M., Suleymanov R.R., Garipov T.T Degradation and remediation of soils polluted with oil‐field wastewater. Eurasian Soil Science, 2013, vol. 46, no. 2, pp. 204‐211. Doi: 10.1134/S1064229313020051</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Souza E.C., Vessoni‐Penna T.C., de Souza Oliveira R.P. Biosurfactant‐enhanced hydrocarbon bioremediation: an overview // International Biodeterioration &amp; Biodegradation. 2014. V. 89. P. 88‐94. Doi: 10.1016/j.ibiod.2014.01.007</mixed-citation><mixed-citation xml:lang="en">Souza E.C., Vessoni‐Penna T.C., de Souza Oliveira R.P. Biosurfactant‐enhanced hydrocarbon bioremediation: an overview. International Biodeterioration &amp; Biodegradation, 2014, vol. 89, pp. 88‐ 94. Doi: 10.1016/j.ibiod.2014.01.007</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Долгова Л.Г. Активность оксидоредуктаз как диагностический показатель загрязнения почвы промышленными отходами // Почвоведение. 1978. N 7. С. 152‐157.</mixed-citation><mixed-citation xml:lang="en">Dolgova L.G. Oxidoreductase activity as a diagnostic indicator of soil contamination with industrial waste. Pochvovedenie [Eurasian Soil Science], 1978, no. 7, pp. 152‐157. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Колесников С.И., Азнаурьян Д.К., Казеев К.Ш., Денисова Т.В. Изучение возможности использования мочевины и фосфогипса в качестве мелиорантов нефтезагрязненных почв в модельном опыте // Агрохимия. 2011. N 9. С. 77‐81.</mixed-citation><mixed-citation xml:lang="en">Kolesnikov S.I., Aznaurʹyan D.K., Kazeev K.Sh., Denisova T.V. Studying the Application of Urea and Phosphogypsum as Ameliorants on Oil‐Contaminated Soils in a Model Experiment. Agrokhimiya [Agrochemistry]. 2011, no. 9, pp. 77‐81. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Новосёлова Е.И. Ферментативная активность почв в условиях нефтяного загрязнения и её биодиагностическое значение // Теоретическая и прикладная экология. 2009. N 2. С. 4‐ 12.</mixed-citation><mixed-citation xml:lang="en">Novosyolova E.I., Kireeva N.A. Soil Enzymatic Activity in Conditions of Oil Contamination and its Biodiagnostic Importance. Teoreticheskaya i prikladnaya ekologiya [Theoretical and Applied Ecology]. 2009, no. 2, pp. 4‐12. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Minnikova T.V., Denisova T.V., Mandzhieva S.S., Kolesnikov S.I., Minkina T.M., Chaplygin V.A., Burachevskaya M.V., Sushkova S.N., Bauer T.V. Assessing the effect of heavy metals from the Novocherkassk power station emissions on the biological activity of soils in the adjacent areas // Journal of Geochemical Exploration. 2017. V. 174. P. 70‐78. Doi: 10.1016/j.gexplo.2016.06.007</mixed-citation><mixed-citation xml:lang="en">Minnikova T.V., Denisova T.V., Mandzhieva S.S., Kolesnikov S.I., Minkina T.M., Chaplygin V.A., Burachevskaya M.V., Sushkova S.N., Bauer T.V. Assessing the effect of heavy metals from the Novocherkassk power station emissions on the biological activity of soils in the adjacent areas. Journal of Geochemical Exploration, 2017, vol. 174, pp.70‐78. Doi: 10.1016/j.gexplo.2016.06.007</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Кочетков И.А., Кавеленова Л.М. Изучение дыхательной активности почв некоторых растительных сообществ Красносамарского лесничества // Материалы межрегиональной научно‐практической конференции «Экологические проблемы среднего Поволжья». Ульяновск. 1999. С. 128‐130.</mixed-citation><mixed-citation xml:lang="en">Kochetkov I.A., Kavelenova L.M. Izuchenie dykhatelʹnoi aktivnosti pochv nekotorykh rastitelʹnykh soobshchestv Krasnosamarskogo lesnichestva [The study of the respiratory activity of the soil of some plant communities of Krasnosamarsky’s forestry]. Materialy mezhregionalʹnoi nauchno‐prakticheskoi konferentsii «Ekologicheskie problemy srednego Povolzhʹya», Ulʹyanovsk, 1999 [Materials of the interregional scientific‐practical conference  ʺEnvironmental problems of the middle Volga regionʺ, Ulʹyanovsk, 1999]. Ulʹyanovsk, 1999, pp. 128‐130. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Каниськин М.А., Терехова В.А., Яковлев А.С. Контроль гуматной детоксикации отходов фосфогипса методами биотестирования // Экология и промышленность России. 2007. N 8. С. 48‐51.</mixed-citation><mixed-citation xml:lang="en">Kanisʹkin M.A., Terekhova V.A., Yakovlev A.S. Control of humate detoxification of phosphogypsum waste using biotesting methods. Ekologia i promyshlennost Rossii [Ecology and Industry of Russia]. 2007, no. 8, pp. 48‐51. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Dindar E., Şağban F.T., Başkaya H.S. Bioremediation of Petroleum‐Contaminated Soil // Journal of Biological and Environmental Sciences. 2013. V. 7. N 19. P. 39‐47.</mixed-citation><mixed-citation xml:lang="en">Dindar E., Şağban F.T., Başkaya H.S. Bioremediation of Petroleum‐Contaminated Soil. Journal of Biological and Environmental Sciences, 2013, vol. 7, no. 19, pp. 39‐47.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Киреева Н.А., Григориади А.С., Хайбуллина Е.Ф. Ассоциации углеводородокисляющих микроорганизмов для биоремедиации нефтезагрязненных почв // Вестник Башкирского университета. 2009. Т. 14. N 2. С. 391‐394.</mixed-citation><mixed-citation xml:lang="en">Kireeva N.A., Grigoriadi A.S., Khaibullina E.F. Association of hydrocarbon‐oxidizing microorganisms for bioremediation of oil‐contaminated soils. Vestnik Bashkirskogo universiteta [Bulletin of Bashkir University]. 2009, vol. 14, no. 2, pp. 391‐394. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Колесников С.И., Казеев К.Ш., Татосян М.Л., Вальков В.Ф. Влияние загрязнения нефтью и нефтепродуктами на биологическое состояние чернозема обыкновенного // Почвоведение. 2006. N 5. С. 616‐620.</mixed-citation><mixed-citation xml:lang="en">Kolesnikov S.I., Kazeev K.Sh., Tatosyan M.L., Valʹkov V.F. The effect of pollution by oil and oil products on the biological status of ordinary chernozems. Pochvovedenie [Eurasian Soil Science]. 2006, no. 5, pp. 552‐556. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Колесников С.И., Казеев К.Ш., Вальков В.Ф., Азнаурьян Д.К., Жаркова М.Г. Биодиагностика экологического состояния почв, загрязненных нефтью и нефтепродуктами. Ростов н/Д: Изд‐во Ростиздат, 2007. 192 с.</mixed-citation><mixed-citation xml:lang="en">Kolesnikov S.I., Kazeev K.Sh., Valʹkov V.F., Aznaurʹyan D.K., Zharkova M.G. Biodiagnostika ekologicheskogo sostoyaniya pochv, zagryaznennykh neftʹyu i nefteproduktami [Biodiagnostics of the ecological condition of soils polluted by oil and oil products]. Rostov‐on‐Done, Rostizdat Publ., 2007, 192 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Agnello A.C., Bagard M., Van Hullebusch E.D., Esposito G., Huguenot D. Comparative bio‐ remediation of heavy metals and petroleum hydrocarbons co-contaminated soil by natural attenuation, phytoremediation, bioaugmentation and bioaugmentation‐assisted phytoremediation // Science of the Total Environment. 2016. V. 563‐564. P. 693‐703. Doi: 10.1016/j.scitotenv.2015.10.061</mixed-citation><mixed-citation xml:lang="en">Agnello A.C., Bagard M., Van Hullebusch E.D., Esposito G., Huguenot D. Comparative bioremediation of heavy metals and petroleum hydrocarbons co‐contaminated soil by natural attenuation, phytoremediation, bioaugmentation and bioaugmentation‐assisted phytoremediation. Science of the Total Environment, 2016, vol. 563‐564, pp. 693‐703. Doi: 10.1016/j.scitotenv.2015.10.061</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Baldan E., Basaglia M., Fontana F., Shapleigh J.P., Casella S. Development, assessment and evaluation of a biopile for hydrocarbons soil remediation // International Biodeterioration &amp; Biodegradation. 2015. V. 98. Р. 66‐72. Doi: 10.1016/j.ibiod.2014.12.002</mixed-citation><mixed-citation xml:lang="en">Baldan E., Basaglia M., Fontana F., Shapleigh J. P., Casella S. Development, assessment and evaluation of a biopile for hydrocarbons soil remediation. International Biodeterioration &amp; Biodegradation, 2015, vol. 98, pp. 66‐72. Doi: 10.1016/j.ibiod.2014.12.002</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Chen M., Xu P., Zeng G., Yang Ch., Huang D., Zhang J. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs // Biotechnology Advances. 2015. V. 33. P. 745‐755. Doi: 10.1016/j.biotechadv.2015.05.003</mixed-citation><mixed-citation xml:lang="en">Chen M., Xu P., Zeng G., Yang Ch., Huang D., Zhang J. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs. Biotechnology Advances, 2015, vol. 33, pp. 745‐755. Doi: 10.1016/j.biotechadv.2015.05.003</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Казеев К.Ш., Колесников С.И., Акименко Ю.В., Даденко Е.В. Методы биодиагностики наземных экосистем. Ростов‐на‐Дону: Издательство ЮФУ, 2016. 356 с.</mixed-citation><mixed-citation xml:lang="en">Kazeev K.Sh., Kolesnikov S.I., Akimenko Yu.V., Dadenko E.V. Metody biodiagnostiki nazemnykh ekosistem [Methods of diagnosis of terrestrial ecosystems]. Rostov‐on‐Don, SFU Publ., 2016, 356 p. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Stepniewska Z., Wolinska A., Ziomek J. Response of soil catalase activity to chromium contamination // J. Environ. Sci. (China). 2009. V. 21. Iss. 8. P. 1142‐1147. Doi: 10.1109/ISWREP.2011.5893614</mixed-citation><mixed-citation xml:lang="en">Stepniewska Z., Wolinska A., Ziomek J. Response of soil catalase activity to chromium con‐ tamination. J. Environ. Sci. (China), 2009, vol. 21, iss. 8, pp. 1142‐1147. Doi: 10.1109/ISWREP.2011.5893614</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Achuba F.I., Peretiemo‐Clarke B.O. Effect of spent engine oil on soil catalase and dehydrogenase activities // International Agrophysics. 2008. N 22. P. 1‐4.</mixed-citation><mixed-citation xml:lang="en">Achuba F.I., Peretiemo‐Clarke B.O. Effect of spent engine oil on soil catalase and dehydrogenase activities. International Agrophysics, 2008, no. 22, pp. 1‐4.</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Bouchez M., Blanchet D., Vandecasteele J‐P. Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism // Applied Microbiology and Biotechnology. 1995. V. 43. Iss. 1. P. 156‐164. Doi: 10.1007/BF00170638</mixed-citation><mixed-citation xml:lang="en">Bouchez M., Blanchet D., Vandecasteele J‐P. Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism. Applied Microbiology and Biotechnology, 1995, vol. 43, iss. 1, pp. 156‐164. Doi: 10.1007/BF00170638</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
